Uruganda Ibicuruzwa bya virusi Magnetic Isaro Uburyo bwa Rna-ADN Acide Nucleic 96 Icyitegererezo cyo gukuramo Tube Kit
Ibisobanuro:
Byihuse usukure ADN ya genomic yo mu rwego rwo hejuru muri bagiteri mugice cyo gukura kwa logarithmic.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Twizera ko ubufatanye burambye mubyukuri ari ibisubizo byujuje ubuziranenge, agaciro kongerewe serivisi, uburambe butera imbere no kumenyana kugiti cyawe Uruganda rwa Virus Magnetic Bead Method Rna-ADN Nucleic Acide 96 Sample Extraction Purification Tube Kit, Twumva ko abakozi bashishikaye, bavunika hasi kandi bahuguwe neza bashobora gushinga amashyirahamwe yubucuruzi akomeye kandi yingirakamaro.Witondere kumva rwose ufite umudendezo wo kutwandikira kubindi bisobanuro.
Twizera ko ubufatanye burebure bwerekana mubyukuri ibisubizo byubuziranenge, agaciro kongerewe serivisi, uburambe butera imbere hamwe numuntu ku giti cyeUbushinwa Nucleic Acide Kit (rna-ADN) na 96 Nucleic Acide, Dushimangiye imiyoborere myiza yumurongo wo hejuru hamwe nabakiriya pro ubufasha, ubu twateguye icyemezo cyacu cyo guha abaguzi bacu dukoresheje itangirana no kubona amafaranga na nyuma ya serivise uburambe bufatika.Gukomeza umubano wubucuti wiganjemo nabaguzi bacu, icyakora dushyashya urutonde rwibisubizo byacu igihe cyose kugirango duhuze ibyifuzo bishya kandi twubahirize iterambere rigezweho ryisoko muri Malta.Twiteguye guhangana n'impungenge no gukora ibishoboka kugirango twumve ibishoboka byose mubucuruzi mpuzamahanga.
Ibisobanuro
50 Itegure, kugirango genomic ADN isukure mumaraso yose ukoresheje <1ml maraso
Buffer DC: Lyses selile zitukura mumaraso.
Buffer BL1: Tanga icyitegererezo cyimyunyu ngugu.
Foregene Protease Yongeyeho: Enzymatique igogora ingero zamaraso mubidukikije bya Buffer BL1.
Buffer BL2: Kora Foregene Protease Plus kandi utange ibidukikije byapakira ADN.
Buffer PW: Kuraho umwanda nka proteyine na RNA muri ADN.
Buffer WB1: Kuraho ion zisigaye muri ADN.
Buffer EB: Hindura ADN kumurongo wo kweza.
ADN-Inkingi Yonyine: Byumwihariko byamamaza ADN genomic muri lysate.
Ibigize ibikoresho
| Buffer BL1 |
| Buffer BL2 * |
| Buffer DC |
| Buffer PW * |
| Buffer WB1 |
| Buffer EB |
| Foregene Protease Yongeyeho |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyigeragezo, ukirinda laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease Plus ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue byihuse;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 40.
-Icyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, 4 ° C centrifugation cyangwa Ethanol imvura ya ADN ntabwo isabwa.
-Umutekano: Nta gukuramo organic reagent ikoreshwa.
-Ubuziranenge bwo hejuru: Ibice bya ADN byakuweho ni binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Birakwiriye gukuramo no kweza ADN genomic mumaraso mashya cyangwa akonje.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
Igisubizo cya Foregene Protease Plus gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);Bizakora cyane kandi bihamye mugihe bibitswe kuri 4 ° C, birasabwa kubibika kuri 4 ° C, twumva ko acid acide Amashyirahamwe asanzwe kandi yingirakamaro mubucuruzi nawe vuba.Witondere kumva rwose ufite umudendezo wo kutwandikira kubindi bisobanuro.
UrugandaUbushinwa Nucleic Acide Kit (rna-ADN) na 96 Nucleic Acide, Dushimangiye imiyoborere myiza yumurongo wo hejuru hamwe nabakiriya pro ubufasha, ubu twateguye icyemezo cyacu cyo guha abaguzi bacu dukoresheje itangirana no kubona amafaranga na nyuma ya serivise uburambe bufatika.Gukomeza umubano wubucuti wiganjemo nabaguzi bacu, icyakora dushyashya urutonde rwibisubizo byacu igihe cyose kugirango duhuze ibyifuzo bishya kandi twubahirize iterambere rigezweho ryisoko muri Malta.Twiteguye guhangana n'impungenge no gukora ibishoboka kugirango twumve ibishoboka byose mubucuruzi mpuzamahanga.
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