Twibwira ko ibyo abaguzi batekereza, byihutirwa gukora mugihe cyinyungu zumuguzi wibitekerezo, kwemerera ibintu byiza cyane byo mu rwego rwo hejuru, kugabanura ibiciro byo gutunganya, kwishyurwa birumvikana, gutsindira abaguzi bashya kandi bishaje igihe cyo gushyigikira no kwemeza Ubushinwa butanga ibicuruzwa bya Fluorescence Igihe nyacyo Rt-PCR Ikizamini cya Reagent Kit kubizamini bya Nucleic Acide, twishimiye cyane uruhare rwanyu mu gihe kiri imbere.
Twibwira ko abaguzi batekereza, byihutirwa gukora mugihe cyinyungu zumuguzi wigitekerezo cya tewolojiya, kwemerera ibintu byiza cyane byo mu rwego rwo hejuru, kugabanya ibiciro byo gutunganya, kwishyurwa birumvikana, gutsindira abaguzi bashya kandi bishaje inkunga no kubyemezaUbushinwa PCR Reagent Kit hamwe na Rt-PCR Ikizamini, Hamwe nintego ya "zero inenge".Kwita kubidukikije, no kugaruka kwabaturage, kwita kubakozi bashinzwe imibereho nkinshingano zabo bwite.Twishimiye inshuti ziturutse impande zose z'isi kudusura no kutuyobora kugirango dushobore kugera ku ntego yo gutsinda.

Ibisobanuro

2 × PCR nyayo byoroshye^TMKuvanga-SYBR yatanzwe nigihe nyacyo PCR Byoroshye^TM-SYBR Icyatsi I kit ni sisitemu nshya ya premix ikoresha SYBR Icyatsi I kuri Real Time PCR amplification reaction, ishobora kuzamura cyane umwihariko wibicuruzwa no kubyumva neza.ROX itangwa nk'irangi ryo kugenzura imbere.Ubwinshi bwa fluorescence yiki gikoresho bwikubye inshuro 3-5 ibyo bicuruzwa bisa, birashobora kuba byoroshye kandi bikagaragaza ubushishozi bwibanze bwa ADN yerekana icyitegererezo.

2 × PCR nyayo yoroshye^TMKuvanga-SYBR irimo Foregene idasanzwe ishyushye-itangira Taq ADN Polymerase.Ugereranije na misemburo isanzwe ya Taq, ifite ibyiza byo gukora neza cyane, ubushobozi bwihariye bwo kongera imbaraga hamwe nigipimo gito kidahuye.Irashobora kugabanya amplification idasanzwe no kunoza ukuri kwa PCR.

Ibisobanuro

Ibiranga ibyiza

Byoroshye - 2X PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora

■ Byihariye - byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer

■ Ibyiyumvo bihanitse - birashobora kumenya kopi nkeya yicyitegererezo

Ver Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR

Porogaramu

Isesengura rya QPCR

Urujya n'uruza rw'akazi

Igishushanyo

Ububiko nubuzima bwiza

Iki gikoresho kigomba kubikwa kure yumucyo kandi kigomba kubikwa kuri -20 ℃.Niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ mugihe gito (iminsi 10).

Twibwira ko ibyo abaguzi batekereza, byihutirwa gukora mugihe cyinyungu zumuguzi wibitekerezo, kwemerera ibintu byiza cyane byo mu rwego rwo hejuru, kugabanura ibiciro byo gutunganya, kwishyurwa birumvikana, gutsindira abaguzi bashya kandi bishaje igihe cyo gushyigikira no kwemeza Ubushinwa butanga ibicuruzwa bya Fluorescence Igihe nyacyo Rt-PCR Ikizamini cya Reagent Kit kubizamini bya Nucleic Acide, twishimiye cyane uruhare rwanyu mu gihe kiri imbere.
Ubushinwa butanga zahabu kuriUbushinwa PCR Reagent Kit hamwe na Rt-PCR Ikizamini, Hamwe nintego ya "zero inenge".Kwita kubidukikije, no kugaruka kwabaturage, kwita kubakozi bashinzwe imibereho nkinshingano zabo bwite.Twishimiye inshuti ziturutse impande zose z'isi kudusura no kutuyobora kugirango dushobore kugera ku ntego yo gutsinda.