Iterambere ryacu rishingiye kubicuruzwa byateye imbere, impano zidasanzwe kandi zongerewe imbaraga muburyo bwikoranabuhanga mugusuzuma ubuziranenge kubushinwa Utm Virus Kits ADN Ikizamini cya Tube Oral Swabs Viral Transport Medium Tubes Nasal Swab Vtm Ki, Mugihe urimo gushakisha uburyo bwiza, butangwa vuba, byiza cyane nyuma yo gushyigikirwa hamwe nogutanga agaciro gakomeye mubushinwa kugirango duhuze ibikorwa byigihe kirekire.
Iterambere ryacu riterwa nibicuruzwa byateye imbere, impano nziza kandi byongerewe imbaraga imbaraga zikoranabuhanga kuriUbushinwa Vtm Tube, Ubusa Vtm 10 Ml Tube, Nkuruganda rufite uburambe natwe twemera gutondekanya ibicuruzwa no kubikora kimwe nifoto yawe cyangwa icyitegererezo cyerekana ibisobanuro hamwe no gupakira abakiriya.Intego nyamukuru yikigo nukubaho kwibuka bishimishije kubakiriya bose, no gushyiraho umubano muremure wubucuruzi.Kubindi bisobanuro, ibuka kutwandikira.Kandi biradushimisha cyane niba ukunda kugira inama kugiti cyawe mubiro byacu.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ mukwezi 1 nyuma yo kongeramo 2-hydroxy-1-ethanethiol (bidashoboka) .Iterambere ryacu riterwa nibicuruzwa byateye imbere, impano nziza kandi byongerewe imbaraga imbaraga zikoranabuhanga mugupima ubuziranenge Ubushinwa Utm Virus Kits ADN Ikizamini cya Tube Oral Swabs Viral Transport Medium Tubes Nasal Swab Vtm Ki, Ubushinwa kumurongo muremure wubucuruzi buciriritse, tuzaba amahitamo yawe meza.
Kugenzura Ubuziranenge kuriUbushinwa Vtm Tube, Ubusa Vtm 10 Ml Tube, Nkuruganda rufite uburambe natwe twemera gutondekanya ibicuruzwa no kubikora kimwe nifoto yawe cyangwa icyitegererezo cyerekana ibisobanuro hamwe no gupakira abakiriya.Intego nyamukuru yikigo nukubaho kwibuka bishimishije kubakiriya bose, no gushyiraho umubano muremure wubucuruzi.Kubindi bisobanuro, ibuka kutwandikira.Kandi biradushimisha cyane niba ukunda kugira inama kugiti cyawe mubiro byacu.