Duhora dushyira mu bikorwa umwuka wo "Guhanga udushya tuzana iterambere, Ubwiza buhebuje butanga ubuzima bwiza, Ubuyobozi bugurisha inyungu, Urutonde rwinguzanyo rukurura abaguzi ku ruganda rwubushinwa kubushinwa Baypure High Yield Tissue Genomic DNA Isukura Kit Reagents Amerika y'Amajyaruguru, Dufite icyemezo cya ISO 9001 kandi twujuje ibyangombwa cyangwa serivise nziza cyane.Murakaza neza ubufatanye natwe!
Duhora dusohoza umwuka wacu "Guhanga udushya tuzana iterambere, Ubwiza buhanitse bwo kubaho, Ubuyobozi bugurisha inyungu, Urutonde rwinguzanyo rukurura abaguzi kuriAutomatic Nucleic Acide Isukura, Ubushinwa Tissue Genomic ADN Kit, Ubwoko bwinshi bwibicuruzwa bitandukanye burahari kugirango uhitemo, urashobora gukora guhaha rimwe.Kandi ibicuruzwa byabigenewe biremewe.Ubucuruzi nyabwo ni ukubona inyungu-zunguka, niba bishoboka, turashaka gutanga inkunga nyinshi kubakiriya.Murakaza neza abaguzi beza bose batugezaho amakuru y'ibicuruzwa natwe !!

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Duhora dushyira mu bikorwa umwuka wo "Guhanga udushya tuzana iterambere, Ubwiza buhebuje butanga ubuzima bwiza, Ubuyobozi bugurisha inyungu, Urutonde rwinguzanyo rukurura abaguzi ku ruganda rwubushinwa kubushinwa Baypure High Yield Tissue Genomic DNA Isukura Kit Reagents Amerika y'Amajyaruguru, Dufite icyemezo cya ISO 9001 kandi twujuje ibyangombwa cyangwa serivise nziza cyane.Murakaza neza ubufatanye natwe!
Uruganda rwo mu Bushinwa kuriUbushinwa Tissue Genomic ADN Kit, Automatic Nucleic Acide Isukura, Ubwoko bwinshi bwibicuruzwa bitandukanye burahari kugirango uhitemo, urashobora gukora guhaha rimwe.Kandi ibicuruzwa byabigenewe biremewe.Ubucuruzi nyabwo ni ukubona inyungu-zunguka, niba bishoboka, turashaka gutanga inkunga nyinshi kubakiriya.Murakaza neza abaguzi beza bose batugezaho amakuru y'ibicuruzwa natwe !!