Ifite ubumenyi bwuzuye mubuhanga bwo kuyobora, ubuziranenge buhebuje ndetse n’idini ryiza cyane, twihesheje izina ryiza kandi twigaruriye uyu murima kubushinwa Ibicuruzwa bishya Ubushinwa Baypure Wholesales Tissue Genomic Nucleic Acid Extraction Kit Reagents Isoko rya Aziya, Mugihe dukoresha amahame y "kwizera gushingiye, kwizera, abakiriya mbere", twakira abakiriya kuri terefone cyangwa bakatwandikira kuri e-mail.
Ifite ubumenyi bwuzuye mubuhanga bwo kuyobora, ubuziranenge buhebuje hamwe n’idini ryiza cyane, tubona izina ryiza kandi twigaruriye uyu murimaAutomatic Nucleic Acide Isukura, Ubushinwa Tissue Genomic ADN Kit, Ibicuruzwa byacu byoherezwa ku isi hose.Abakiriya bacu bahora banyuzwe nubwiza bwizewe, serivisi zishingiye kubakiriya nibiciro byapiganwa.Inshingano yacu "ni ugukomeza kubona ubudahemuka bwacu twitangira imbaraga zacu kugirango duhore tunoza ibicuruzwa na serivisi byacu kugirango tumenye neza abakoresha bacu ba nyuma, abakiriya, abakozi, abatanga isoko ndetse n’umuryango mpuzamahanga dufatanya".

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Ifite ubumenyi bwuzuye mubuhanga bwo kuyobora, ubuziranenge buhebuje ndetse n’idini ryiza cyane, twihesheje izina ryiza kandi twigaruriye uyu murima kubushinwa Ibicuruzwa bishya Ubushinwa Baypure Wholesales Tissue Genomic Nucleic Acid Extraction Kit Reagents Isoko rya Aziya, Mugihe dukoresha amahame y "kwizera gushingiye, kwizera, abakiriya mbere", twakira abakiriya kuri terefone cyangwa bakatwandikira kuri e-mail.
Ubushinwa Ibicuruzwa bishyaUbushinwa Tissue Genomic ADN Kit, Automatic Nucleic Acide Isukura, Ibicuruzwa byacu byoherezwa ku isi hose.Abakiriya bacu bahora banyuzwe nubwiza bwizewe, serivisi zishingiye kubakiriya nibiciro byapiganwa.Inshingano yacu "ni ugukomeza kubona ubudahemuka bwacu twitangira imbaraga zacu kugirango duhore tunoza ibicuruzwa na serivisi byacu kugirango tumenye neza abakoresha bacu ba nyuma, abakiriya, abakozi, abatanga isoko ndetse n’umuryango mpuzamahanga dufatanya".