Bikaba bifite ubumenyi bwuzuye bwo kuyobora, ubuhanga buhebuje hamwe n’idini ryiza cyane, twihesheje izina ryiza kandi twigaruriye uyu murima kubikoresho byubushinwa Ubushinwa Bwiza Bwiza Bihendutse Ibihe Byiza bya PCR Ikizamini cya Laboratoire ya Laboratoire, Niba ushishikajwe nibicuruzwa byacu cyangwa wifuza kuvuga kubiguzi byabugenewe, ugomba kumva rwose ufite umudendezo wo kudufata.
Ifite ubumenyi bwuzuye mubuhanga bwo kuyobora, ubuziranenge buhebuje hamwe n’idini ryiza cyane, tubona izina ryiza kandi twigaruriye uyu murimaGutahura Acide Nucleic Acide, Ubushinwa Rt PCR IbikoreshoIsosiyete yacu, ihora ijyanye nubuziranenge nkishingiro ryisosiyete, ishakisha iterambere binyuze murwego rwo hejuru rwizewe, yubahiriza amahame yimicungire yubuziranenge ya iso9000, ishyiraho isosiyete yo mu rwego rwo hejuru ikoresheje umwuka ugaragaza ubunyangamugayo nicyizere.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi yuzuye ya RNA yuzuye mubice bitandukanye byibimera bifite polysaccharide cyangwa polifenol nyinshi.Itanga ADN-Isukura inkingi ishobora gukuraho byoroshye ADN ya genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
■ By'umwihariko bikwiranye no kweza RNA mu ngero z'ibimera bya polysaccharide na polifenol.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ample Icyitegererezo: Ibimera bishya cyangwa bikonje bya polysaccharide na polifenol
Ingano: 50mg tissue yibihingwa
Ubushobozi ntarengwa bwa RNA bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nyinshi hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko hamwe nubuzima bwa Shelf

Iki gikoresho gishobora kubikwa amezi 24 mugihe cyumye mubushyuhe bwicyumba (15-25 ℃);niba ikeneye kubikwa igihe kirekire, irashobora kubikwa muri 2-8 ℃.
Buffer PSL1 irashobora gushirwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo β-mercaptoethanol (birasabwa kongeramo mugihe kimwe cyubushakashatsi).

Bikaba bifite ubumenyi bwuzuye bwo kuyobora, ubuhanga buhebuje hamwe n’idini ryiza cyane, twihesheje izina ryiza kandi twigaruriye uyu murima kubikoresho byubushinwa Ubushinwa Bwiza Bwiza Bihendutse Ibihe Byiza bya PCR Ikizamini cya Laboratoire ya Laboratoire, Niba ushishikajwe nibicuruzwa byacu cyangwa wifuza kuvuga kubiguzi byabugenewe, ugomba kumva rwose ufite umudendezo wo kudufata.
Ubushinwa bw'umwugaUbushinwa Rt PCR Ibikoresho, Gutahura Acide Nucleic AcideIsosiyete yacu, ihora ijyanye nubuziranenge nkishingiro ryisosiyete, ishakisha iterambere binyuze murwego rwo hejuru rwizewe, yubahiriza amahame yimicungire yubuziranenge ya iso9000, ishyiraho isosiyete yo mu rwego rwo hejuru ikoresheje umwuka ugaragaza ubunyangamugayo nicyizere.