Inkomoko y'Ubushinwa Ubushinwa Rt-PCR Ubushuhe bwa Zahabu-X ADN Polymerase hamwe na PCR Buffer Ihingura nuwitanga |Foregene

Inkomoko y'uruganda Ubushinwa Rt-PCR Ubushyuhe bwa Zahabu-X ADN Polymerase hamwe na PCR Buffer

Ibisobanuro:

◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)

◮Ibikoresho bike

◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya

◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura

◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50

◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Guhanga udushya, ubuziranenge kandi bwizewe nindangagaciro shingiro ryishirahamwe ryacu.Aya mahame uyumunsi arenze ikindi gihe cyose aricyo shingiro ryibyo twagezeho nkikigo mpuzamahanga giciriritse gikora hagati yinganda zikora uruganda Ubushinwa Rt-PCR Thermostable Gold-X ADN Polymerase hamwe na PCR Buffer, "Ishyaka, Kuba inyangamugayo, gushyigikira amajwi, ubufatanye bukomeye niterambere" ni gahunda zacu.Twabaye hano dutegereje inshuti nziza kwisi yose!
Guhanga udushya, ubuziranenge kandi bwizewe nindangagaciro shingiro ryishirahamwe ryacu.Aya mahame uyumunsi yiyongereye kuruta ikindi gihe cyose shingiro ryibyo twatsinze nkurwego mpuzamahanga ruciriritse ruciriritse kuriUbushinwa PCR, Qpcr, Hamwe numwuka w "inguzanyo mbere, iterambere binyuze mu guhanga udushya, ubufatanye buvuye ku mutima no gutera imbere hamwe", isosiyete yacu irihatira gushyiraho ejo hazaza heza hamwe nawe, kugirango ibe urubuga rwiza rwo kohereza ibicuruzwa byacu mubushinwa!

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Imbuto y'ibihingwa Direct PCR Kit I.
Igice I.	Buffer SP1
	Buffer SP2
	6 Buffer Yipakurura ADN
Igice cya II	2 × Imbuto PCR byoroshye^TMKuvanga

Imbuto y'ibihingwa Direct PCR Kit II
Igice I.	Buffer SP1
	Buffer SP2
	6 Buffer Yipakurura ADN
Igice cya II	2 × Imbuto PCR byoroshye^TMKuvanga

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN

Material Ibikoresho bike

■ Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya

X 2X Kuvanga-kugabanya amakosa yo gupakira ibintu hamwe nigihe cyo gutegura sisitemu

■ Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50

Thr Kwinjira cyane - lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Kumenyekanisha ibimera byahinduwe, genotyping, nibindi.

Icyitegererezo: Tera imbuto cyangwa tissue zifite polysaccharide nkeya hamwe na polifenol.

Ingano yicyitegererezo: Imbuto imwe yose, 1-100mg yumubiri wibimera

Urutonde rwo kumenya: ibicuruzwa bya PCR ≤1kb

Urujya n'uruza rw'akazi

gutera imbuto PCR

Amashanyarazi

imbuto yibihingwa PCR-DIAGRAM Guhanga udushya, ubuziranenge kandi bwizewe nindangagaciro shingiro ryishirahamwe ryacu.Aya mahame uyumunsi arenze ikindi gihe cyose aricyo shingiro ryibyo twagezeho nkikigo mpuzamahanga giciriritse gikora hagati yinganda zikora uruganda Ubushinwa Rt-PCR Thermostable Gold-X ADN Polymerase hamwe na PCR Buffer, "Ishyaka, Kuba inyangamugayo, gushyigikira amajwi, ubufatanye bukomeye niterambere" ni gahunda zacu.Twabaye hano dutegereje inshuti nziza kwisi yose!
Inkomoko y'urugandaUbushinwa PCR, Qpcr, Hamwe numwuka w "inguzanyo mbere, iterambere binyuze mu guhanga udushya, ubufatanye buvuye ku mutima no gutera imbere hamwe", isosiyete yacu irihatira gushyiraho ejo hazaza heza hamwe nawe, kugirango ibe urubuga rwiza rwo kohereza ibicuruzwa byacu mubushinwa!