Ibicuruzwa byuruganda Ubushinwa Baybio Bbex32 Ubushobozi Bwinshi Bwikora Automatic Nucleic Acide Gukuramo Ibikoresho Byubuvuzi
Ibicuruzwa byacu byubahwa cyane kandi byizewe nabakoresha amaherezo kandi birashobora guhura noguhindura ibyifuzo byimari nimbonezamubano bikenerwa nu ruganda rwo mu Bushinwa Baybio Bbex32 Ikora neza cyane Automatic Nucleic Acide Extraction Platform ibikoresho byubuvuzi, Abakiriya bacu bakwirakwijwe cyane muri Amerika y'Amajyaruguru, Afurika n'Uburayi bw'Uburasirazuba.turashobora kubona byoroshye ibisubizo byujuje ubuziranenge hamwe nigiciro cyiza cyo gutera.
Ibicuruzwa byacu byubahwa cyane kandi byizewe nabakoresha amaherezo kandi birashobora guhura noguhora duhindura imari nibisabwaAutomatic Nucleic Acide Isukura, Ubushinwa Nucleic Acide, Isosiyete yacu itanga intera yuzuye kuva mbere yo kugurisha kugeza kuri serivisi nyuma yo kugurisha, kuva iterambere ryibicuruzwa kugeza kugenzura imikoreshereze yabyo, hashingiwe ku mbaraga zikomeye za tekiniki, imikorere y’ibicuruzwa byiza, ibiciro byiza na serivisi nziza, tuzakomeza kwiteza imbere, gutanga ibintu na serivisi byujuje ubuziranenge, kandi dutezimbere ubufatanye burambye n’abakiriya bacu, iterambere rusange kandi dushyireho ejo hazaza heza.
Kits Ibisobanuro
50 Imyiteguro, 200 Imyiteguro
Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.
Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.
Ibigize ibikoresho:
Buffer RL1, Buffer RL2 |
Buffer RW1, Buffer RW2 |
RNase-Yubusa ddH2O, Inkingi-Yeza |
RNA-Inkingi gusa |
Ibiranga ibyiza
■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1
Porogaramu
Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.
Ibipimo byibicuruzwa
Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 106
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl
Urujya n'uruza rw'akazi
Igishushanyo
Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar
Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima
Ububiko nubuzima bwiza
Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).
Ibicuruzwa byacu byubahwa cyane kandi byizewe nabakoresha amaherezo kandi birashobora guhura noguhindura ibyifuzo byimari nimbonezamubano bikenerwa nu ruganda rwo mu Bushinwa Baybio Bbex32 Ikora neza cyane Automatic Nucleic Acide Extraction Platform ibikoresho byubuvuzi, Abakiriya bacu bakwirakwijwe cyane muri Amerika y'Amajyaruguru, Afurika n'Uburayi bw'Uburasirazuba.turashobora kubona byoroshye ibisubizo byujuje ubuziranenge hamwe nigiciro cyiza cyo gutera.
Ahantu hacururizwaUbushinwa Nucleic Acide, Automatic Nucleic Acide Isukura, Isosiyete yacu itanga intera yuzuye kuva mbere yo kugurisha kugeza kuri serivisi nyuma yo kugurisha, kuva iterambere ryibicuruzwa kugeza kugenzura imikoreshereze yabyo, hashingiwe ku mbaraga zikomeye za tekiniki, imikorere y’ibicuruzwa byiza, ibiciro byiza na serivisi nziza, tuzakomeza kwiteza imbere, gutanga ibintu na serivisi byujuje ubuziranenge, kandi dutezimbere ubufatanye burambye n’abakiriya bacu, iterambere rusange kandi dushyireho ejo hazaza heza.