Igiciro Cyiza Mubushinwa Byihuta Byihuse-Igihe Cyuzuye PCR Imashini Igiciro PCR Kugurisha hamwe na CE Icyemezo
Dutsimbaraye ku ihame ryawe rya "Serivisi nziza nziza, Serivise ishimishije", Turimo guharanira kubona umufatanyabikorwa wubucuruzi utangaje wumushinga wawe kubiciro byiza kubushinwa Ibihe Byihuse Byihuta-Igihe Cyuzuye PCR Imashini Igiciro PCR Kugurisha hamwe na CE Icyemezo, Ibicuruzwa byakozwe nibyiza byamamaza.Twitabira cyane kubyara no kwitwara mubunyangamugayo, hamwe nabakiriya bacu murugo no mumahanga kuva muruganda rwa xxx.
Twumiye ku ihame ryawe rya "Serivisi nziza nziza, serivisi ishimishije", Turimo guharanira kubona umufatanyabikorwa wubucuruzi utangaje kuriweGutahura Acide Nucleic Acide, Ubushinwa Rt PCR Ibikoresho, Izina ryisosiyete, buri gihe ireba ubuziranenge nkishingiro ryisosiyete, ishakisha iterambere binyuze murwego rwo hejuru rwizewe, yubahiriza amahame yimicungire yubuziranenge ya ISO, ishyiraho isosiyete yo mu rwego rwo hejuru ikoresheje umwuka wo kwerekana ubunyangamugayo nicyizere.
Kits Ibisobanuro
50 Imyiteguro, 200 Imyiteguro
Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.
Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.
Ibigize ibikoresho:
Buffer RL1, Buffer RL2 |
Buffer RW1, Buffer RW2 |
RNase-Yubusa ddH2O, Inkingi-Yeza |
RNA-Inkingi gusa |
Ibiranga ibyiza
■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1
Porogaramu
Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.
Ibipimo byibicuruzwa
Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 106
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl
Urujya n'uruza rw'akazi
Igishushanyo
Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar
Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima
Ububiko nubuzima bwiza
Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).
Dutsimbaraye ku ihame ryawe rya "Serivisi nziza nziza, Serivise ishimishije", Turimo guharanira kubona umufatanyabikorwa wubucuruzi utangaje wumushinga wawe kubiciro byiza kubushinwa Ibihe Byihuse Byihuta-Igihe Cyuzuye PCR Imashini Igiciro PCR Kugurisha hamwe na CE Icyemezo, Ibicuruzwa byakozwe nibyiza byamamaza.Twitabira cyane kubyara no kwitwara mubunyangamugayo, hamwe nabakiriya bacu murugo no mumahanga kuva muruganda rwa xxx.
Igiciro cyiza kuriUbushinwa Rt PCR Ibikoresho, Gutahura Acide Nucleic Acide, Izina ryisosiyete, buri gihe ireba ubuziranenge nkishingiro ryisosiyete, ishakisha iterambere binyuze murwego rwo hejuru rwizewe, yubahiriza amahame yimicungire yubuziranenge ya ISO, ishyiraho isosiyete yo mu rwego rwo hejuru ikoresheje umwuka wo kwerekana ubunyangamugayo nicyizere.