Hamwe na siyansi yuzuye yubuyobozi bwiza, ubuziranenge buhebuje hamwe n’ukwizera gukomeye, twabonye umwanya mwiza cyane kandi twigaruriye uru ruganda imyaka 18 Uruganda Ubushinwa Virus ADN / Rna Pre-Package Extraction Kit ya 96automated Nucleic Acide Extraction Laboratory, dushobora gukemura ibibazo byabakiriya bacu asap kandi tugakorera inyungu kubakiriya bacu.Ukeneye isosiyete nziza kandi yujuje ubuziranenge, pls uduhitemo, urakoze!
Hamwe na siyansi yuzuye yuburyo bwiza bwo kuyobora, ubwiza buhebuje hamwe no kwizera gukomeye, tubona umwanya mwiza cyane kandi twigaruriye ingandaGukuramo Ubushinwa Reagent, Virusi Dna Rna Ikuramo, Turashobora guhaza ibyifuzo bitandukanye byabakiriya murugo no mumahanga.Twakiriye neza abakiriya bashya nabakera kuza kugisha inama no kuganira natwe.Guhazwa kwawe nibyo bitera imbaraga!Reka dufatanye kwandika igice gishya cyiza!

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Hamwe na siyansi yuzuye yubuyobozi bwiza, ubuziranenge buhebuje hamwe n’ukwizera gukomeye, twabonye umwanya mwiza cyane kandi twigaruriye uru ruganda imyaka 18 Uruganda Ubushinwa Virus ADN / Rna Pre-Package Extraction Kit ya 96automated Nucleic Acide Extraction Laboratory, dushobora gukemura ibibazo byabakiriya bacu asap kandi tugakorera inyungu kubakiriya bacu.Ukeneye isosiyete nziza kandi yujuje ubuziranenge, pls uduhitemo, urakoze!
Uruganda rwimyaka 18Gukuramo Ubushinwa Reagent, Virusi ya ADN Rna Ikuramo Kit, Turashobora guhaza ibyifuzo bitandukanye byabakiriya murugo no mumahanga.Twakiriye neza abakiriya bashya nabakera kuza kugisha inama no kuganira natwe.Guhazwa kwawe nibyo bitera imbaraga!Reka dufatanye kwandika igice gishya cyiza!