Ubushinwa uburyo bwihuse bwo kwigunga no kweza RNA yose kuva selile Inganda nuwitanga |Foregene

uburyo bwihuse bwo kwigunga no kweza RNA yose kuva selile

Ibisobanuro:

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe;Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Sisitemu yose ni RNase-Yubusa, kugirango RNA isukuye ntisuzugurwe;Buffer RW1, Buffer RW2 sisitemu yo gukaraba ya garanti ya RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda w’ibinyabuzima.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Twishimiye ko abaguzi banyuzwe kandi bakemerwa cyane kuberako dukomeje gushakisha ubuziranenge haba murwego rwo gukemura no gutanga serivisi kuburyo bwihuse bwo kwigunga no kweza RNA yose mu tugari, Dukurikiza filozofiya ntoya y'ubucuruzi ya 'umukiriya wa 1, tera imbere', twakira neza abakiriya baturutse iwanyu ndetse no mumahanga kugirango badufatanye natwe.
Twishimiye ko abaguzi banyuzwe kandi bakemerwa cyane kubera guhora dukurikirana ubuziranenge haba murwego rwo gukemura no gutanga serivisi kuriuburyo bwihuse bwo kwigunga no kweza RNA yose kuva selile zifite umuco, Isosiyete yacu ikora ikurikiza ihame ryibikorwa by "ubunyangamugayo bushingiye, ubufatanye bwashyizweho, abantu berekeza, ubufatanye-bunguka".Turizera ko dushobora kugirana ubucuti numucuruzi uturutse impande zose zisi.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Buffer cRL1, Buffer cRL2

Buffer RW1, Buffer RW2

Ubusa ddH₂O, Inkingi-yoza

RNA-Inkingi gusa

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

selile yose RNA

Igishushanyo

Akagari Igiteranyo Cyuzuye RNA Igikoresho Cyakazi Flow1

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) na 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka) .Twishimiye ko ushimishijwe cyane nabaguzi kandi ko twakiriwe neza kubera ko twakomeje gushakisha ubuziranenge haba murwego rwo gukemura no gutanga serivisi mugihe gito cyo mu Bushinwa Imyaka 2 Garanti yinganda zikora inganda zitwara abagenzi mu mahanga igipimo natwe.
Igihe gito cyo kuyobora Ubushinwa Powder Coating Booth, Inganda zikora inganda, Isosiyete yacu ikora ihame ryibikorwa by "uburinganire bushingiye ku bufatanye, ubufatanye bwashyizweho, abantu berekeza, ubufatanye-bunguka".Turizera ko dushobora kugirana ubucuti numucuruzi uturutse impande zose zisi.

Mbere: byuzuye bya reagent

Ibikurikira: Biroroshye gukoresha CELL TOTAL RNA ISOLATION KIT

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