Twifashishije sisitemu yuzuye yubuyobozi bufite ireme, ireme ryiza cyane hamwe no kwizera gusumba byose, dutsindira igihagararo cyiza kandi dukurikiza iyi disipuline kubiciro bidahenze byinganda Ubushinwa Long Taq ADN Polymerase, Long PCR, Twakiriye byimazeyo abashyitsi bose kugirango bategure umubano wibigo natwe dushingiye kubintu byiza byombi.Ugomba kutwandikira nonaha.Urashobora kubona igisubizo cyubuhanga imbere yamasaha 8.
Twifashishije sisitemu yuzuye yubuyobozi bwiza bufite ireme, ubuziranenge bwiza kandi kwizera gusumba, dutsindira igihagararo cyiza kandi dukurikiza iyi disipuliniUbushinwa PCR, Qpcr, Isosiyete yacu ifite ubuso bwa metero kare 20 000 000.Dufite abakozi barenga 200, itsinda ryabahanga mu bya tekinike, uburambe bwimyaka 15, gukora neza, gukora neza kandi kwizewe, igiciro cyapiganwa nubushobozi buhagije bwo gukora, nuburyo dukomeza abakiriya bacu.Niba ufite ikibazo, nyamuneka ntutindiganye kutwandikira.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Amafi ya Zebra nandi mafi yamazi meza.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer FP reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

v Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

vReagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

vReagent 2 × PCR EasyTM ivanze, iyo ikoreshejwe kenshi, irashobora kandi kubikwa kuri 4 ℃ kugirango ibike igihe gito (ikoreshwa muminsi 10) .Gukoresha sisitemu yuzuye yubuyobozi bwiza bufite ireme, ubuziranenge bwiza kandi kwizera gukomeye, dutsindira igihagararo cyiza kandi dukoresha iyi disipuline kubiciro biciriritse Ubushinwa Long Taq ADN Polymerase, Long PCR, Twakiriye neza abashyitsi bose kugirango dushyire hamwe umubano mwiza mubufatanye bwiza.Ugomba kutwandikira nonaha.Urashobora kubona igisubizo cyubuhanga imbere yamasaha 8.
uruganda igiciro gitoUbushinwa PCR, Qpcr, Isosiyete yacu ifite ubuso bwa metero kare 20 000 000.Dufite abakozi barenga 200, itsinda ryabahanga mu bya tekinike, uburambe bwimyaka 15, gukora neza, gukora neza kandi kwizewe, igiciro cyapiganwa nubushobozi buhagije bwo gukora, nuburyo dukomeza abakiriya bacu.Niba ufite ikibazo, nyamuneka ntutindiganye kutwandikira.