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Inkomoko y'uruganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Acide Nucleic Acide Kit Rna Kwigunga ADN Ikuramo ibikoresho bya PCR Ngs

Inkomoko y'uruganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Acide Nucleic Acide Kit Rna Kwigunga ADN Ikuramo ibikoresho bya PCR Ngs Ikiranga
  • Inkomoko y'uruganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Acide Nucleic Acide Kit Rna Kwigunga ADN Ikuramo ibikoresho bya PCR Ngs
  • Inkomoko y'uruganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Acide Nucleic Acide Kit Rna Kwigunga ADN Ikuramo ibikoresho bya PCR Ngs
  • Inkomoko y'uruganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Acide Nucleic Acide Kit Rna Kwigunga ADN Ikuramo ibikoresho bya PCR Ngs
  • Inkomoko y'uruganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Acide Nucleic Acide Kit Rna Kwigunga ADN Ikuramo ibikoresho bya PCR Ngs

Ibisobanuro:

Iki gikoresho gikoresha ikoranabuhanga rya Real-RT RT (rRT-PCR) kugirango hamenyekane neza SARS-CoV-2 (ORF1ab gene na N gene) nucleic acide muri nasofaryngeal cyangwa oropharyngeal swab sample, hakoreshejwe uburyo bwa multiplex PCR fluorescent probe.Iki gikoresho kirimo igenzura ryimbere kugirango risuzume ubwiza bwikigereranyo, bityo ntigikenewe kwipimisha nuclée nini,


Ibicuruzwa birambuye

Ibicuruzwa

Fata inshingano zuzuye zo guhaza ibyo abakiriya bacu bakeneye byose;kugera ku majyambere ahoraho ushimangira kwagura abaguzi bacu;hindukira mubufatanye bwa nyuma bwa koperative yumukiriya kandi wongere inyungu zabakiriya kumasoko yinganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Nucleic Acide Gukuramo Kit Rna Isolation ADN Ikuramo ibikoresho bya PCR Ngs, Ibicuruzwa byose bikozwe nibikoresho bigezweho hamwe nuburyo bukomeye bwa QC kugirango harebwe ubuziranenge.Murakaza neza kubakiriya bashya nabakera kutwandikira mubufatanye mubucuruzi.
Fata inshingano zuzuye zo guhaza ibyo abakiriya bacu bakeneye byose;kugera ku majyambere ahoraho ushimangira kwagura abaguzi bacu;hindukira mubufatanye bwanyuma bwa koperative yumukiriya kandi wongere inyungu zabakiriya kuriUbushinwa Nucleic Acide Gukuramo Reagent Kit, Kit Nucleic Acide Gukuramo Reagent Kit, Nicyitegererezo gikomeye kandi gitezimbere neza kwisi yose.Ntuzigere ubura ibikorwa byingenzi mugihe cyihuse, ni ngombwa mugihe cyawe cyiza cyiza.Kuyoborwa nihame rya “Ubushishozi, Gukora neza, Ubumwe no guhanga udushya.isosiyete.ake imbaraga zidasanzwe zo kwagura ubucuruzi mpuzamahanga, kuzamura ishyirahamwe.rofit no kuzamura igipimo cyo kohereza hanze.Twizeye ko tugiye kugira ibyiringiro byiza no gukwirakwizwa kwisi yose mumyaka iri imbere.


Ibisobanuro

48 Rxns, 96 Rxns

Oya.

Ibigize

Umubare

Ibice nyamukuru

48 Rxns

96 Rxns
1 Nucleic aside irekura 1.4mL / tube Imiyoboro 2 5.3 mL / icupa Icupa 1 Surfactant
2 Kurinda RNA 27 μL / umuyoboro 1 tube 53 μL / umuyoboro 1 tube RNase inhibitor
3 SARS-CoV-2 igisubizo cyibisubizo 800 μL / umuyoboro 1 tube 1600 μL / umuyoboro 1 tube Primer, probe, reaction reaction, dNTP
4 SARS-CoV-2 enzyme ivanze 80 μL / umuyoboro 1 tube 160 μL / umuyoboro 1 tube Gutangira bishyushye Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 kugenzura neza 100 μL / umuyoboro 1 tube 100 μL / umuyoboro 1 tube Recombinant plasmid ikubiyemo igice cyagenewe, RNA
6 SARS-CoV-2 kugenzura nabi 1200 μL / umuyoboro 1 tube 1200 μL / umuyoboro 1 tube TE buffer

Ibiranga ibyiza

Byihuse kandi byoroshye.
Irinde gutakaza aside nucleic.
Umupaka muto wo gutahura no kwiyumvamo byinshi.
Ibikoresho bike bisabwa no gukoresha byoroshye.

Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).

GCE / mL Ct agaciro
N gen ORF1ab
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 N / A. N / A.
2.00E-01 N / A. N / A.
NC N / A. N / A.

Umurongo wo kwongera umurongo

amplification curve-RT-qPCR

Uburyo bwo gukoresha

1. Igikoresho gikoreshwa

covid-19 nucleic aside itahura kit1

2. Uburyo bwo gutahura

covid-19 nucleic aside itahura kit2

Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (gukora intambwe 1 gusa) (Tegereza iminota 10)

PCR amplificaton (<= 55min)

Urupapuro rw'akazi:

covid-19 nucleic aside itahura kit4

Ububiko

bifunze ku mucyo kandi bibitswe kuri -20 ± 5 ℃;

ububiko
ububiko2

Ubuzima bwa Shelf:Umwaka 1

Fata inshingano zuzuye zo guhaza ibyo abakiriya bacu bakeneye byose;kugera ku majyambere ahoraho ushimangira kwagura abaguzi bacu;hindukira mubufatanye bwa nyuma bwa koperative yumukiriya kandi wongere inyungu zabakiriya kumasoko yinganda Ubushinwa Magnetic Bead Method Saliva Swab Icyitegererezo cya Nucleic Acide Gukuramo Kit Rna Isolation ADN Ikuramo ibikoresho bya PCR Ngs, Ibicuruzwa byose bikozwe nibikoresho bigezweho hamwe nuburyo bukomeye bwa QC kugirango harebwe ubuziranenge.Murakaza neza kubakiriya bashya nabakera kutwandikira mubufatanye mubucuruzi.
Inkomoko y'urugandaUbushinwa Nucleic Acide Gukuramo Reagent Kit, Kit Nucleic Acide Gukuramo Reagent Kit, Nicyitegererezo gikomeye kandi gitezimbere neza kwisi yose.Ntuzigere ubura ibikorwa byingenzi mugihe cyihuse, ni ngombwa mugihe cyawe cyiza cyiza.Kuyoborwa nihame rya “Ubushishozi, Gukora neza, Ubumwe no guhanga udushya.isosiyete.ake imbaraga zidasanzwe zo kwagura ubucuruzi mpuzamahanga, kuzamura ishyirahamwe.rofit no kuzamura igipimo cyo kohereza hanze.Twizeye ko tugiye kugira ibyiringiro byiza no gukwirakwizwa kwisi yose mumyaka iri imbere.

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