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Kugurisha Byinshi Mubushinwa Antigen 1 Intambwe Yihuta Ikizamini Antigen Byihuta Ikizamini Urugo Ikizamini cyo Gukoresha Rimwe

Ibisobanuro:

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer ishobora kurekura byihuse RNA mumikorere yimikorere yimikorere ya RT-qPCR, bityo bikuraho inzira yo kweza RNA itwara igihe kandi ikora cyane.Inyandikorugero ya RNA irashobora kuboneka muminota 7 gusa.5 × Direct RT ivanze na 2 × Imiyoboro ya qPCR ivanze-SYBR itangwa nigitabo irashobora kwihuta kandi neza kubona ibisubizo nyabyo-byuzuye bya PCR.

5 × Direct RT ivanze na 2 × Direct qPCR ivanze-SYBR ifite kwihanganira imbaraga za inhibitor, kandi lysate yintangarugero irashobora gukoreshwa nkicyitegererezo cya RT-qPCR itaziguye.Iki gikoresho kirimo RNA idasanzwe-yuzuye ya Foregene reverse transcriptase, hamwe na Hoteri D-Taq ADN polymerase, DNTPs, MgCl2, reaction reaction, PCR optimizer na stabilisateur.


Ibicuruzwa birambuye

Ibicuruzwa

Turabizi ko dutera imbere gusa niba tuzemeza ko duhiganwa hamwe nibiciro byujuje ubuziranenge hamwe n’inyungu nziza icyarimwe icyarimwe cyo kugurisha neza Ubushinwa Antigen 1 Intambwe Yihuta Ikizamini cya Antigen Rapid Ikizamini cyo mu rugo Ikizamini cyo Gukoresha Rimwe, Twishimiye byimazeyo abaguzi bo mu mahanga kugira ngo bagire inama kuri ubwo bufatanye bw'igihe kirekire hiyongereyeho iterambere.
Turabizi ko dutera imbere gusa niba tuzemeza ko duhurije hamwe ibiciro byapiganwa kandi bifite ireme ryiza icyarimweIgikoresho cyihuta cyUbushinwa, Ikizamini cya Pcr, Tumaze imyaka irenga 20 dukora ibicuruzwa byacu.Ahanini mukore byinshi, dufite igiciro cyapiganwa cyane, ariko cyiza.Mu myaka yashize, twabonye ibisubizo byiza cyane, atari ukubera ko dutanga ibicuruzwa byiza, ariko nanone kubera serivisi nziza nyuma yo kugurisha.Turi hano tugutegereje kubibazo byawe.

Ibisobanuro

200 × 20μl Rxns, 1000 × 20μl Rxns

Ibigize ibikoresho

Igice cya I.

Buffer CL

Foregene Protease Yongeyeho II

Buffer ST

Igice cya II

Gusiba ADN

5 × Direct RT ivanze

2 × Direct qPCR ivanze-SYBR

50 × ROX Yerekana irangi

RNase Yubusa ddH2O

Amabwiriza

Ibiranga ibyiza

■ Byoroshye kandi byiza: hamwe na tekinoroji ya Direct Direct RT, ingero za RNA zirashobora kuboneka muminota 7 gusa.

Icyitegererezo gikenewe ni gito, nkuko selile 10 zishobora kugeragezwa.

Thr Kwinjiza cyane: irashobora kumenya vuba RNA mu ngirabuzimafatizo zifite 384, 96, 24, 12, 6-amasahani meza.

Er ADN Eraser irashobora gukuraho vuba genome zasohotse, bikagabanya cyane ingaruka kubisubizo byakurikiyeho.

System Optimized RT na qPCR sisitemu ituma intambwe ebyiri RT-PCR ihinduranya transcript neza kandi PCR ikarushaho kuba nziza, kandi ikarwanya RT-qPCR inhibitor.

Porogaramu

Igipimo cyo gushyira mu bikorwa: ingirabuzimafatizo.

- RNA yarekuwe na sample lysis: ikoreshwa gusa kuri RT-qPCR inyandikorugero yiki gitabo.

- Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: gusesengura imvugo ya gene, kugenzura ingaruka zo gucecekesha gene ya siRNA, gusuzuma ibiyobyabwenge, nibindi.

Igishushanyo

Akagari kayobora RT qPCR igishushanyo

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 4 ℃;Igice cya II kigomba kubikwa kuri -20 ℃.

Foregene Protease Plus II igomba kubikwa kuri 4 ℃, ntugahagarike kuri -20 ℃.

Reagent 2 × Direct qPCR Ivanga-SYBR igomba kubikwa kuri -20 ℃ mu mwijima;biramutse bikoreshejwe kenshi, birashobora kandi kubikwa kuri 4 ℃ kubikwa mugihe gito (koresha muminsi 10) .Tuzi ko dutera imbere gusa niba tuzemeza ko duhurijwe hamwe hamwe nibiciro byujuje ubuziranenge icyarimwe mugihe cyo kugurisha neza Ubushinwa Antigen 1 Intambwe Yihuta Ikizamini Antigen Rapid Test Strip Home Ikizamini cyo Gukoresha Rimwe, Turakira byimazeyo abaguzi bo mumahanga kugira ngo tugire inama kubufatanye bwigihe kirekire.
Kugurisha ByizaIgikoresho cyihuta cyUbushinwa, Ikizamini cya PCR, Tumaze imyaka irenga 20 dukora ibicuruzwa byacu.Ahanini mukore byinshi, dufite igiciro cyapiganwa cyane, ariko cyiza.Mu myaka yashize, twabonye ibisubizo byiza cyane, atari ukubera ko dutanga ibicuruzwa byiza, ariko nanone kubera serivisi nziza nyuma yo kugurisha.Turi hano tugutegereje kubibazo byawe.


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