PCR Reagent
Komisiyo yacu igomba kuba iyo gukorera abakoresha bacu n'abaguzi ba nyuma bafite ubuziranenge bwo hejuru kandi burushanwe ibicuruzwa bigendanwa kandi bigashakirwa ibisubizoPCR Reagent, Twishimiye byimazeyo abafatanyabikorwa bombi bo mu mahanga ndetse n’abashoramari bo mu gihugu imbere, kandi twizera ko tuzakorana nawe mu gihe kirekire!
Komisiyo yacu igomba kuba iyo gukorera abakoresha bacu n'abaguzi ba nyuma bafite ubuziranenge bwo hejuru kandi burushanwe ibicuruzwa bigendanwa kandi bigashakirwa ibisubizoPCR Reagent, Hamwe nuburambe bwimyaka 30 mubucuruzi, twizeye serivisi nziza, ubuziranenge no gutanga.Twakiriye neza abakiriya baturutse impande zose zisi kugirango bafatanye nisosiyete yacu mugutezimbere.
Ibisobanuro
5ml, 10ml, 40ml
Ibigize ibikoresho
0.1U Intwari ya Taq / μl |
400μM DNTP |
20mM Tris-HCl (pH8.8) |
100mM KCl |
5mM MgCl2 |
Izindi stabilisateur hamwe nabazamura |
Ububiko bwa ADN |
Ibiranga ibyiza
Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;
Speed Umuvuduko wihuse wihuta: umuvuduko wa amplification ni 5-10sec / kb, wikubye inshuro 6-12 ugereranije na Taq enzyme isanzwe;
Ibindi byitegererezo byo guhuza n'imiterere: Irashobora kwagura neza inyandikorugero zitandukanye za ADN hamwe na hejuruIbirimo GC;
Efficiency Gukora neza cyane: kugabanuka kwinshi kurenza imisemburo ya Taq isanzwe;
Erance Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;
Ifite ibikorwa 5 ′ → 3 ′ ibikorwa bya ADN polymerase na 5 ′ → 3 ′ ibikorwa bya exonuclease, nta 3 ′ → 5 ′ ibikorwa bya exonuclease.
Kit Ibikoresho byabanjirijwe kuvangwa na Electrophoresis Loading Buffer, PCR imaze kurangira, icyitegererezo gishobora gutwarwa na electrophorei;imikorere iroroshye kandi yoroshye.
Porogaramu
Kwiyongera kwa PCR ibice bya ADN, ibimenyetso bya ADN, uko ADN ikurikirana, PCR wongeyeho umurizo
Ububiko hamwe nubuzima bwa Shelf
Irashobora kubikwa igihe kirekire kuri -20 ° C;kugirango ikoreshwe kenshi, irashobora kubikwa kuri 4 ° C mugihe gito. 2 × Real PCR EasyTM Mix-SYBR itangwa na Real Time PCR EasyTM-SYBR Green I kit ni sisitemu nshya ya premix ikoresha SYBR Icyatsi I kuri Real Time PCR amplification reaction, ishobora kuzamura cyane umwihariko wibicuruzwa no kubyumva neza.ROX itangwa nk'irangi ryo kugenzura imbere.Ubwinshi bwa fluorescence yiki gikoresho bwikubye inshuro 3-5 ibyo bicuruzwa bisa, birashobora kuba byoroshye kandi bikagaragaza ubushishozi bwibanze bwa ADN yerekana icyitegererezo.
2 × PCR nyayo EasyTM ivanze-SYBR ikubiyemo Foregene idasanzwe ishyushye-itangira Taq DNA Polymerase.Ugereranije na misemburo isanzwe ya Taq, ifite ibyiza byo gukora neza cyane, ubushobozi bwihariye bwo kongera imbaraga hamwe nigipimo gito kidahuye.Irashobora kugabanya amplification idasanzwe no kunoza ukuri kwa PCR.