Nuburyo bwo kukugezaho byoroshye no kwagura ikigo cyacu, dufite kandi abagenzuzi mubakozi ba QC kandi nkwizeza isosiyete yacu nibicuruzwa byiza byurugero rwubusa.Ubushinwa Acide Nucleic Acide (ADN / RNA) Miniprep Kit, Virus Rna Gukuramo Magnetic Isaro V4002, 200 Preps, Dutegereje gushiraho amashyirahamwe yigihe kirekire yubucuruzi hamwe nawe.Amagambo yawe nibyifuzo byawe birashimwa rwose.
Nuburyo bwo kubagezaho byoroshye no kwagura ikigo cyacu, dufite kandi abagenzuzi mubakozi ba QC kandi nkwizeza isosiyete yacu nibicuruzwa byiza kuri(ADN / RNA) Miniprep Kit, Ubushinwa Acide Nucleic Acide, Dutanga serivisi zujuje ibisabwa, gusubiza byihuse, gutanga ku gihe, ubuziranenge bwiza nigiciro cyiza kubakiriya bacu.Guhazwa ninguzanyo nziza kuri buri mukiriya nibyo dushyira imbere.Twibanze kuri buri kantu ko gutunganya ibicuruzwa kugeza igihe bakiriye ibicuruzwa byiza kandi byiza hamwe na serivisi nziza y'ibikoresho hamwe nigiciro cyubukungu.Ukurikije ibi, ibicuruzwa byacu bigurishwa neza cyane mubihugu byo muri Afrika, Uburasirazuba bwo hagati na Aziya yepfo yepfo.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yujuje ubuziranenge RNA yose mubice bitandukanye byibimera bifite polysaccharide nkeya hamwe na polifenol.Kubitegererezo byibihingwa bifite polysaccharide nyinshi cyangwa polifenole, birasabwa gukoresha Plant Total RNA Isolation Plus Kit kugirango ubone ibisubizo byiza byo gukuramo RNA.Igikoresho gitanga ADN-Isukura inkingi ishobora gukuraho ADN genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nkeya hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) ahantu humye, na 2-8 ℃ mugihe kirekire (amezi 24).

Buffer PSL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-ethanethiol (bidashoboka) .Nuburyo bwo kukugezaho byoroshye no kwagura ikigo cyacu, dufite n'abagenzuzi mubakozi ba QC kandi tukwizeza ko uruganda rwacu rwiza nibicuruzwa byuruganda rwubusa Urugero rwa Virus Nucleic Acide(ADN / RNA) Miniprep Kit, Virus Rna Gukuramo Magnetic Isaro V4002, 200 Preps, Dutegereje gushiraho amashyirahamwe yigihe kirekire yubucuruzi hamwe nawe.Amagambo yawe nibyifuzo byawe birashimwa rwose.
Uruganda ntangarugero Ubushinwa Virus Nucleic Acide, (ADN / RNA) Miniprep Kit, Dutanga serivisi zujuje ibisabwa, igisubizo cyihuse, gutanga ku gihe, ubuziranenge bwiza nigiciro cyiza kubakiriya bacu.Guhazwa ninguzanyo nziza kuri buri mukiriya nibyo dushyira imbere.Twibanze kuri buri kantu ko gutunganya ibicuruzwa kugeza igihe bakiriye ibicuruzwa byiza kandi byiza hamwe na serivisi nziza y'ibikoresho hamwe nigiciro cyubukungu.Ukurikije ibi, ibicuruzwa byacu bigurishwa neza cyane mubihugu byo muri Afrika, Uburasirazuba bwo hagati na Aziya yepfo yepfo.