Twishimiye kwamamara kwiza bidasanzwe mubakiriya bacu kubicuruzwa byacu byiza cyane ibicuruzwa byujuje ubuziranenge, bikaze ndetse ninkunga ifatika kubatanga isoko ryambere Ubushinwa Automated PCRGukuramo Acide NucleicSisitemu, Ibikoresho byo Gukuramo Acide Nucleic, Umutekano nkibisubizo byo guhanga udushya nibyo dusezerana.
Twishimiye kwamamara kwiza bidasanzwe mubakiriya bacu kubicuruzwa byacu byiza cyane, igiciro cyiza ndetse nubufasha bukomeye kuriUbushinwa Imashini ikuramo aside Nucleic, Gukuramo Acide Nucleic, Dutanga serivise yumwuga, gusubiza byihuse, gutanga ku gihe, ubuziranenge bwiza nigiciro cyiza kubakiriya bacu.Guhazwa ninguzanyo nziza kuri buri mukiriya nibyo dushyira imbere.Twibanze kuri buri kantu ko gutunganya ibicuruzwa kugeza igihe baboneye ibisubizo byiza kandi byiza hamwe na serivisi nziza y'ibikoresho hamwe nigiciro cyubukungu.Ukurikije ibi, ibicuruzwa byacu nibisubizo bigurishwa neza cyane mubihugu byo muri Afrika, Uburasirazuba bwo hagati na Aziya yepfo yepfo.Twisunze filozofiya yubucuruzi ya ?? abakiriya mbere, tera imbere ', twakira byimazeyo abakiriya baturutse mu gihugu ndetse no hanze kugirango bafatanye natwe.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka) .Turabona umunezero wo kwamamara kwiza bidasanzwe mubakiriya bacu kubicuruzwa byacu byiza cyane ibicuruzwa byujuje ubuziranenge, bikaze ndetse nubufasha bukomeye kubatanga isoko ryambere Ubushinwa Automated PCRGukuramo Acide NucleicSisitemu, Ibikoresho byo Gukuramo Acide Nucleic, Umutekano nkibisubizo byo guhanga udushya nibyo dusezerana.
Abatanga isokoUbushinwa Imashini ikuramo aside NucleicGukuramo Acide Nucleic, Dutanga serivise yumwuga, igisubizo cyihuse, gutanga ku gihe, ubuziranenge bwiza nigiciro cyiza kubakiriya bacu.Guhazwa ninguzanyo nziza kuri buri mukiriya nibyo dushyira imbere.Twibanze kuri buri kantu ko gutunganya ibicuruzwa kugeza igihe baboneye ibisubizo byiza kandi byiza hamwe na serivisi nziza y'ibikoresho hamwe nigiciro cyubukungu.Ukurikije ibi, ibicuruzwa byacu nibisubizo bigurishwa neza cyane mubihugu byo muri Afrika, Uburasirazuba bwo hagati na Aziya yepfo yepfo.Twisunze filozofiya yubucuruzi ya ?? abakiriya mbere, tera imbere ', twakira byimazeyo abakiriya baturutse mu gihugu ndetse no hanze kugirango bafatanye natwe.