Ubushinwa nucleic aside yipimisha nicyumba cyicyitegererezo Cyakozwe nuwitanga |Foregene

gupima aside nucleic nicyumba cyo gutoranya

gupima aside nucleic hamwe nicyitegererezo Icyumba cyerekanwe

Ibisobanuro:

Iki gikoresho gikoresha ikoranabuhanga rya Real-RT RT (rRT-PCR) kugirango hamenyekane neza SARS-CoV-2 (ORF1ab gene na N gene) nucleic acide muri nasofaryngeal cyangwa oropharyngeal swab sample, hakoreshejwe uburyo bwa multiplex PCR fluorescent probe.Iki gikoresho kirimo igenzura ryimbere kugirango risuzume ubwiza bwikigereranyo, bityo ntigikenewe kwipimisha nuclée nini,

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Intego yacu igomba kuba uguhuriza hamwe no kuzamura ubuziranenge na serivisi byibicuruzwa biriho, hagati aho dutezimbere ibicuruzwa bishya nibisubizo kugirango twuzuze ibyo abakiriya bakeneye.gupima aside nucleic nicyumba cyo gutoranya, Turasezeranye kugerageza ibishoboka byose kugirango tuguhe ubuziranenge buhebuje kandi ibisubizo byiza.
Intego yacu igomba kuba uguhuriza hamwe no kuzamura ubuziranenge na serivisi byibicuruzwa biriho, hagati aho dutezimbere ibicuruzwa bishya nibisubizo kugirango twuzuze ibyo abakiriya bakeneye.gupima aside nucleic nicyumba cyo gutoranya, Hamwe nuburambe bwimyaka irenga 9 hamwe nitsinda ryumwuga, ubu twohereje ibisubizo byacu mubihugu byinshi n'uturere twinshi kwisi.Twishimiye abakiriya, amashyirahamwe yubucuruzi ninshuti ziturutse impande zose zisi kutwandikira no gushaka ubufatanye kubwinyungu rusange.

Ibisobanuro

48 Rxns, 96 Rxns

Oya.	Ibigize	Umubare				Ibice nyamukuru
Oya.	Ibigize	48 Rxns		96 Rxns		Ibice nyamukuru
1	Nucleic aside irekura	1.4mL / tube	Imiyoboro 2	5.3 mL / icupa	Icupa 1	Surfactant
2	Kurinda RNA	27 μL / umuyoboro	1 tube	53 μL / umuyoboro	1 tube	RNase inhibitor
3	SARS-CoV-2 igisubizo cyibisubizo	800 μL / umuyoboro	1 tube	1600 μL / umuyoboro	1 tube	Primer, probe, reaction reaction, dNTP
4	SARS-CoV-2 enzyme ivanze	80 μL / umuyoboro	1 tube	160 μL / umuyoboro	1 tube	Gutangira bishyushye Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 kugenzura neza	100 μL / umuyoboro	1 tube	100 μL / umuyoboro	1 tube	Recombinant plasmid ikubiyemo igice cyagenewe, RNA
6	SARS-CoV-2 kugenzura nabi	1200 μL / umuyoboro	1 tube	1200 μL / umuyoboro	1 tube	TE buffer

Ibiranga ibyiza

Byihuse kandi byoroshye.
Irinde gutakaza aside nucleic.
Umupaka muto wo gutahura no kwiyumvamo byinshi.
Ibikoresho bike bisabwa no gukoresha byoroshye.

Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).

GCE / mL	Ct agaciro
GCE / mL	N gen	ORF1ab
2.00E + 08	16.43	15.16
2.00E + 07	19.48	18.58
2.00E + 06	22.71	21.86
2.00E + 05	25.95	25.33
2.00E + 04	29.30	28.49
2.00E + 03	32.37	31.37
2.00E + 02	35.32	34.47
2.00E + 01	38.29	38.27
2.00E + 00	N / A.	N / A.
2.00E-01	N / A.	N / A.
NC	N / A.	N / A.

Umurongo wo kwongera umurongo

amplification curve-RT-qPCR

Uburyo bwo gukoresha

1. Igikoresho gikoreshwa

covid-19 nucleic aside itahura kit1

2. Uburyo bwo gutahura

covid-19 nucleic aside itahura kit2

Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (gukora intambwe 1 gusa) (Tegereza iminota 10)

PCR amplificaton (<= 55min)

Urupapuro rw'akazi:

covid-19 nucleic aside itahura kit4

Ububiko

bifunze ku mucyo kandi bibitswe kuri -20 ± 5 ℃;

ububiko
ububiko2

Ubuzima bwa Shelf:Umwaka 1

Intego yacu igomba kuba uguhuriza hamwe no kuzamura ubuziranenge na serivisi byibicuruzwa biriho, hagati aho, dutezimbere kenshi ibicuruzwa bishya nibisubizo kugirango twuzuze ibyifuzo byabakiriya ku ruganda rutangwa nu Bushinwa FDA Sterile Medical Nylon Flock Flocked Sampling Specimen Collection Throat Nasopharyngeal Flocked Swabs Liquid Transport Medium, Turasezeranya ko tuzagerageza gukora ibishoboka byose kugirango tuguhe ibisubizo byiza kandi byiza.
Uruganda rwatanze Ubushinwa Swab, Igikoresho cyo gutera inshinge, Hamwe nuburambe bwimyaka irenga 9 hamwe nitsinda ryabigize umwuga, ubu twohereje ibisubizo byacu mubihugu byinshi n'uturere twinshi kwisi.Twishimiye abakiriya, amashyirahamwe yubucuruzi ninshuti ziturutse impande zose zisi kutwandikira no gushaka ubufatanye kubwinyungu rusange.

Mbere: Uruganda rwa OEM / ODM Ubushinwa Vtm Ikoreshwa rya virusi Ikwirakwizwa rya Nilon Nasal Umuhogo Swab

Ibikurikira: Icyitegererezo cyubusa kubushinwa CE Nucleic Acide ADN / Rna Ikuramo Kit + Igikoresho

Andika ubutumwa bwawe hano hanyuma utwohereze