Amakuru yinganda
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Igishushanyo mbonera-cyerekana umwihariko, gutuza no gukora neza
Umwihariko wo gutahura Mubihe byinshi, intego yo gushushanya primer ni ukugaragaza umwihariko wa PCR.Ibi bigenwa ningaruka nyinshi cyangwa nkeya zishobora guhanurwa zimpinduka nyinshi.Impinduka imwe yingenzi ni urukurikirane kuri 3′end ya primer.Icyangombwa, PCR isuzuma yagenewe ...Soma byinshi -
Imiti ya acide nucleique yinjiye mugihe cya zahabu, ni ubuhe buryo bw'ingenzi bugomba gukemurwa byihutirwa?
Inkomoko: Micro Medical Medical Nyuma y’icyorezo cya COVID-19, inkingo ebyiri za mRNA zemejwe vuba kugira ngo zimenyekanishe, ibyo bikaba byaratumye abantu benshi bashishikazwa no guteza imbere imiti ya aside nucleique.Mu myaka yashize, imiti myinshi ya acide nucleic ifite ubushobozi bwo kuba imiti igabanya ubukana yashyize ahagaragara ...Soma byinshi -
Isesengura ryerekana ibimenyetso byerekana ibibazo bya Primer mugihe kibanza cya PCR Reagents
Kugenzura imikorere ya primers na probe mubyiciro byambere bya reagent ya PCR no kumenya uburyo bwiza bwo kubyitwaramo nibisabwa kugirango habeho iterambere ryibigeragezo bisanzwe.Nigute dukeneye kwemeza primer probe kare ...Soma byinshi -
Gutoranya ingingo zubumenyi abagenzuzi bagomba kubona
Kwipimisha muri laboratoire bitangirana no gukusanya icyitegererezo, kandi icyegeranyo nticyoroshye kwirengagiza.Ikintu cyingenzi cyo gukusanya icyitegererezo ni uguhitamo ubwoko bwicyitegererezo gikwiye, gukoresha ibikoresho byikitegererezo, no gukora ubwikorezi no gutunganya neza.I.Urugero rw'ubwoko Rusange sam ...Soma byinshi -
Igisubizo cyanyuma cyo kwanduza aside nucleic aside
Uburyo bwa PCR hamwe na acide nucleic aside aerosol yanduye muri laboratoire yo gupima aside nucleic ni nkimpande ebyiri z'igiceri.Turashobora guhitamo kubigira cyangwa kutabikora, ariko ntidushobora guhitamo niba tubishaka cyangwa kubikoresha.1. Kugaragaza ikurwaho rya ADN Kugirango ugere ahantu hava ...Soma byinshi -
Kumenyekanisha byihuse ibimera bya transgenji
Nka shyashya muri laboratoire, ntabwo ari akazi keza kugenzura ibihingwa byiza bivuye kumurima wibimera bifite igipimo gito cyo guhinduka.Ubwa mbere, ADN igomba gukurwa mububare bwinshi bw'icyitegererezo umwe umwe, hanyuma genes zo mumahanga zikamenyekana na PCR.Ariko, ibisubizo akenshi usanga ari ubusa ...Soma byinshi -
Ibisobanuro byuzuye byingingo zingenzi za test ya acide nucleic SARS-CoV-2, kuki hariho ibibi bibi nibisubirwamo?
Mugihe cyambere cyicyorezo, kubera iterambere ryihuse, gusuzuma byihuse abarwayi bakekwa nurufunguzo rwo kwirinda COVID-19.Bimwe mu byemezo bya nucleic aside byerekana reagent bifite igihe gito cyiterambere, kandi hariho ibibazo nko kwemeza imikorere byihuse, rea idahagije ...Soma byinshi -
SNP ni iki? Ingingo zerekeye genetiki yabaturage
Inyuguti eshatu SNP zirahari hose mukwiga genetika yabaturage.Hatitawe ku bushakashatsi bwindwara zabantu, imiterere yibihingwa, ubwihindurize bwinyamaswa hamwe n’ibidukikije bya molekuline, SNPs irakenewe nkibanze.Ariko, niba udafite ubushishozi bwimbitse bwubwoko bugezweho ...Soma byinshi -
Nigute ushobora kunoza imikorere ya LncRNA ihinduranya inyandiko?
Ibiranga lncRNA: 1. lncRNAs mubisanzwe ni birebire, hamwe nimvugo ifite imbaraga nuburyo butandukanye bwo gutandukanya mugihe cyo gutandukana;2. Ugereranije na coding genes, lncRNA mubisanzwe iba hasi;3. LncRNAs nyinshi zifite imvugo yigihe gito nu mwanya igaragara mubikorwa ...Soma byinshi -
Ibisubizo bine byingenzi kuri PCR Igenzura ryangiza ibicuruzwa
1 ： Simbuza ibikoresho byubushakashatsi mugihe Gushiraho (NTC) kugenzura nabi hanyuma ubisubiremo inshuro nyinshi.Bimaze kugaragara ko muri PCR hari ibicuruzwa byanduye muri laboratoire, simbuza ibikoresho byose byubushakashatsi mugihe.Nka: ongera ushushe kandi utegure primers, ongera uhindure inama ya pipette, E ...Soma byinshi -
Imikorere ibiri-ikora ya RT-PCR
Imyandikire gakondo ntishobora kwihanganira ubushyuhe bwo hejuru (ubushyuhe bwiza kubikorwa bya MMLV ni 37-50 ° C, na AMV ni 42-60 ° C).Virusi irushijeho gukomera RNA ntishobora guhindurwa neza muri cDNA mubushyuhe buke, bikavamo gukora neza Kugabanuka.Tra ...Soma byinshi -
Nucleic aside isothermal amplification tekinoroji
PCR nubuhanga bukoreshwa cyane muri nucleic aside amplification kandi ikoreshwa cyane kubera sensibilité yayo kandi yihariye.Ariko, PCR isaba guhindagurika inshuro nyinshi kandi ntishobora gukuraho imbogamizi zo kwishingikiriza ku bikoresho n'ibikoresho, bigabanya ikoreshwa ryayo mu mavuriro ...Soma byinshi
