Ibiranga lncRNA:

1. lncRNAs mubisanzwe ni birebire, hamwe nuburyo bugaragara hamwe nuburyo butandukanye bwo gutandukanya mugihe cyo gutandukana;

2. Ugereranije na coding genes, lncRNA mubisanzwe iba hasi;

3. LncRNAs nyinshi zifite imiterere yigihe gito nigitekerezo cyihariye muburyo bwo gutandukanya ibice niterambere;

4. Hariho imvugo iranga ibibyimba nizindi ndwara;

5. Ibibanza bito bya lncRNA biratandukanye;

6. Urukurikirane ruri hasi mukubungabunga, ariko imikorere ifite urwego runaka rwo kubungabunga nibindi.

LncRNA ihindura igisubizo cyinyandiko:

Turabizi ko lncRNA ifite urwego rwohejuru rwimiterere muri selile.Bene RNA ikunze kugira imiterere igoye nka stem loop cyangwa umusatsi.Niba imiterere ya kabiri ya lncRNA idashobora gukemurwa neza, transcript ihindagurika ikunda gutsindwa cyangwa kunanirwa mugihe ukora RT-qPCR.Ikibazo cyo gukora neza.

Mubisanzwe, hari ingingo eshatu zingenzi kuri twe kugirango tubone inyandikorugero nziza ya cDNA:

Inyandikorugero ya RNA, ihinduranya ryibanze rya primers hamwe na transcript transcriptase, na transcript transcriptase nibintu byingenzi byingenzi.RNA yahanaguwe na reagent ikunze gukoreshwa ya RNA ikunze kuba irimo ibisigazwa byinzoga hamwe nu munyu wa guanidine, bigira ingaruka zikomeye zo kubuza kwanduza inyandiko nyinshi;niba ubushyuhe bwiza kuri reaction transcriptase reaction iri hasi, ntabwo bizafasha RNA.Gufungura imiterere yubuyobozi.

Kugeza ubu, ubushyuhe bukwiye bwa transcriptase ikoreshwa muri laboratoire nyinshi muri rusange ni 42 ° C, kandi ubushyuhe ntarengwa bushobora kugera kuri 50 ° C.Mubyukuri, gufungura urwego rwohejuru rwa RNA imiterere bisaba ubushyuhe bwo hejuru.

Ubushyuhe bwo guhuza umurongo wa mbere wa cDNA ya Fortune Biotech 2 × RT EasyTM ivanze ni hejuru ya 50 ℃ -65 ℃, ifasha gukuramo burundu imiterere yo murwego rwohejuru rwa lncRNA, kandi igihe cyo gukora ni gito.

Byongeye kandi, ibicuruzwa bifite n'ubushobozi bukomeye bwo kurwanya-kwivanga kuri RNA hamwe n'inzoga zisigaye hamwe n'umunyu wa guanidine, bishobora guhaza byimazeyo ibikenewe muri laboratoire kugirango bigerweho neza.

Igice cya mbere cDNA Synthesis Kit

Ingingo Oya: RT-01011 (inshuro 25)

Ibiranga:

1. Ubushyuhe bwumuriro: 65 ℃ buracyafite ibikorwa byiza byo kwandukura inyandiko, bifasha gufungura urwego rwisumbuye rwa kabiri

2. Ubworoherane: synthesis ya cDNA irashobora gukorwa neza kuri templates ya RNA ifite isuku nke (ibisigazwa byumunyu wa Ethanol na guanidine)

3. Byihuse: imikorere yoroshye, umurongo wambere cDNA synthesis irashobora kurangira muminota 25-35

Porogaramu zo hasi: PCR, qPCR, ISOKO, kuranga chip, kubaka isomero, nibindi.

Byongeye kandi, FJ Biotech itanga ibisubizo byuzuye bya RT-PCR kugirango ihuze ibyifuzo byubwoko butandukanye bwabakiriya, harimo nibikoresho bikurikira:

Igice cya mbere cDNA Synthesis Kit (Kuri qPCR)

Ingingo Oya: RT-01021 (inshuro 50)

Ibiranga:

1. Icyoroshye: Revers transcription primers yongeyeho kugirango imikorere yoroshye

2. Ubushyuhe bwumuriro: 65 ℃ buracyafite ibikorwa byiza byo kwandukura inyandiko, bifasha gufungura urwego rwisumbuye rwa kabiri

3. Ubworoherane: cDNA synthesis irashobora gukorwa neza kurugero rwa RNA hamwe nubuziranenge bubi

4. Ibyiyumvo: bikwiranye na RNA ntoya

Porogaramu yo hepfo: Byatanzwe byumwihariko kuri qPCR ikurikira

Intambwe imwe ya RT-PCR kit

Ingingo Oya: RT-02011 (inshuro 100)

Ibiranga:

1. Ubushyuhe bwumuriro: 65 ℃ buracyafite ibikorwa byiza byo kwandukura inyandiko, bifasha gufungura urwego rwisumbuye rwa kabiri

2. Ubworoherane: cDNA synthesis irashobora gukorwa neza kurugero rwa RNA hamwe nubuziranenge bubi

3. Umwihariko: PCR MIX ifite ibikoresho bitezimbere neza imikorere ya amplification nuburyo bwihariye bwa reaction

Porogaramu yo hasi: cDNA ikoronije, ibizamini bya genetike nubundi bushakashatsi bwa molekile

Intambwe ebyiri RT-PCR kit

Ingingo Oya: RT-02021 (inshuro 100)

Ibiranga:

1. Byoroshye kandi byihuse: reaction imwe

2. Ubushyuhe bwumuriro: 65 ℃ buracyafite ibikorwa byiza byo kwandukura inyandiko, bifasha gufungura urwego rwisumbuye rwa kabiri

3. Umwihariko: Wongeyeho Foregene Taq itezimbere neza imikorere ya amplification hamwe nibisobanuro bya reaction

Porogaramu zo hasi: ubushakashatsi bwa molekuline nko gupima genetike

Intambwe imwe RT-qPCR kit-SYBR Icyatsi I.

Ingingo No: RT-02111 (inshuro 100)

Ibiranga:

1. Byoroshye: reaction imwe

2. Ubukangurambaga bukabije: bushobora kumenya kopi ntoya

3. Guhuza: bikwiranye nibikoresho byinshi bya fluorescence

Porogaramu yo hasi: gutahura ingero za RNA nka selile na virusi

Intambwe imwe RT-qPCR kit-Taqman

Ingingo No: RT-02131 (inshuro 100)

Ibiranga:

1. Byihuse: menya gene igenewe

2. Ubukangurambaga bukabije: bushobora kumenya kopi ntoya

3. Guhuza: bikwiranye nibikoresho byinshi bya fluorescence

Porogaramu yo hasi: gutahura ingero za RNA nka selile na virusi
Guhimba Bioreverse Transcription Kit irashobora kugufasha gukemura neza ikibazo cyo kwandukura.Muri icyo gihe, ikorana na Forge Bio RNA Extraction Kit (ishobora gukuraho ibisigazwa bya ADN) kugirango iguhe igisubizo cyuzuye cya RNA.