Uburyo bwa PCR hamwe na acide nucleic aside aerosol yanduye muri laboratoire yo gupima aside nucleic ni nkimpande ebyiri z'igiceri.Turashobora guhitamo kubigira cyangwa kutabikora, ariko ntidushobora guhitamo niba tubishaka cyangwa kubikoresha.

1. Kwerekana gukuraho ADN

Kugirango ukureho umwanya wa acide nucleic acide aerosol yanduye, birakenewe mbere na mbere gusuzuma ibyakuweho ADN bishobora gukuraho aside nucleic mumazi.Kuberako nta gukuraho ADN byinshi bikora rwose.Kuburyo bwubushakashatsi, nyamuneka reba: Gukuraho ADN ntibishobora kuba "imfuruka y'ibanga" ya laboratoire!

Muri ubu bushakashatsi, kopi 100 / μL (CT hafi 31) ya ASFV plasmid na ADN ikuraho ADN yagereranijwe na PCR ya PCR yavanze mubunini bungana, hanyuma ikora mubushyuhe bwicyumba cya 10min, 20min na 30min.Nyuma yo gukuramo aside nucleic, kwongera qPCR byakozwe.Igenzura ryiza rivanze na plasmid namazi byagereranijwe.Kubera ko igeragezwa ritarangiye icyarimwe, hashobora kubaho gutandukana hagati y ibisubizo, ariko ntabwo bigira ingaruka kumusozo wubushakashatsi.Kugeza ubu, nasuzumye ibicuruzwa 10 bivanaho ADN.Ibicuruzwa No 1, No 6 na No 8 byonyine birashobora gutesha agaciro ADN ya plasmide muburyo bwamazi.Ibindi bicuruzwa nta nkurikizi bifite.

Imbonerahamwe 1 Ingaruka zo gukuraho aside Nucleic yo gukuraho ADN ikuraho ubucuruzi

2. Ikigereranyo cyo gukuraho ADN igeragezwa ryakunze gukoreshwa

1. Kwerekana imiti yica udukoko

Hariho ubwoko bwinshi bwa disinfectant zikoreshwa muri laboratoire: aldehydes, fenol, alcool, umunyu wa amonium wa kane, peroxide, imyiteguro ya chlorine, na acide na base.Ingaruka zo kwanduza izo disinfectant kuri mikorobe zagenzuwe neza nubushakashatsi, ariko nta makuru yubushakashatsi ahagije afite ku ngaruka zo kwangirika kwa acide nucleique.Laboratoire zipima aside nucleique zikenera kwanduza indwara zidafite ingaruka nziza zo kwanduza mikorobe gusa, ariko kandi zishobora no kwangiza ADN.Ibisubizo by'ibizamini byerekana ko imyiteguro ibiri ya chlorine yonyine ya aside 84 yica udukoko na trichloroisocyanuric na aside 1 M hydrochloric yujuje ibisabwa.Kubera ko aside 1 M hydrochloric yangirika cyane, birasabwa ko imyiteguro ya chlorine yakoreshwa nko guta imyanda muri laboratoire yo gupima aside nucleic.Nyamara, imyiteguro ya chlorine yangirika ku byuma kandi ntishobora gukoreshwa mu kwanduza ibikoresho n'ibikoresho.

Imbonerahamwe 2 Ingaruka zo gukuraho ADN zubwoko butandukanye bwangiza

2. Nibura byibuze imbaraga za chlorine zitegura

Imiti yica udukoko twa Chlorine igira ingaruka zikomeye zo kwangirika kwa ADN, ariko kubera kwangirika kwicyuma no kurakara, zirashobora gukoreshwa mugusukura hasi, ibyuma bidafite ibyuma, inama zokunywa, imiyoboro ya centrifuge nibindi bikoresho byipimisha.

Dukurikije "Amabwiriza ya Tekinike y’icyorezo cya Epidemic Disinfection New Coronary Pneumonia": Umwanda uhumanya (amaraso y’abarwayi, ururenda no kuruka) uraboneka chlorine 5g / L-10g / L irimo chlorine irimo imiti yica udukoko;amagorofa, urukuta, hamwe nubuso bwibintu bikoresha chlorine iboneka 1g / L Chlorine yangiza: imyenda, uburiri nizindi myenda babanza gushiramo imiti yica chlorine hamwe na 0.5g / L biboneka muri chlorine muminota 30, hanyuma bigasukurwa nkuko bisanzwe.

Imbonerahamwe 3 Ingaruka zo gukuraho ADN yibintu bitandukanye bya chlorine yangiza

Ibisubizo by'ibizamini byerekana ko: iyo kwibumbira hamwe kwa chlorine birenze cyangwa bingana na 1,2 g / L, imiti yica chlorine irashobora kwangiza burundu kopi 100 / μL plasmid iyo ikoreshejwe muminota 5.Iyo ukora muminota 10, 84 yanduza hamwe na chlorine ikora neza irenze cyangwa ihwanye na 0,6 g / L irashobora gutesha agaciro kopi 100 / μL za plasima.

3. Ikigeragezo cyo gukuraho ikirere cyandujwe na nucleic aside aerosol

Nigute ushobora kweza umwuka wanduye na acide nucleic acide?Byinshi mubitekerezo ni ugukora ibidukikije byanduye aerosol hanyuma ukabisukura, cyangwa guhanagura aside nucleic nyuma yo kwanduzwa.Kuri njye, igeragezwa ridashobora gusubirwamo kandi ibisubizo byubushakashatsi ntibishobora gusesengurwa kubwinshi ntacyo bivuze, nuko rero natije uburyo bumwe na bumwe bwo kwanduza ikirere.Reba "GB27948-2020 Ibisabwa muri rusange byangiza imiti yangiza ikirere" na "Ibisobanuro bya tekiniki byangiza"

1. Ibikoresho by'ibizamini

1.1 Gukuraho ADN: Muri ubu bushakashatsi, hatoranijwe ADN ebyiri zikoreshwa mu bucuruzi 6 na 8 mu mbonerahamwe ya 1.

1.2 Ikizamini cya plasmid: Urebye ko CT agaciro kanduye muri laboratoire kazaba hejuru ya 30, kwibumbira hamwe kwa ASFV gene plasmide yakoreshejwe muriki gihe ni kopi 100 / μL, naho PCR CT igereranya 31.07.Ongeramo plasmid kumpamba zipakurura kandi umwuka wumye.

1.3 Igikoresho cyo kwipimisha: Igikoresho cyo kwanduza Bilingkehan KVBOX, gishyigikirwa neza na Shenzhen Runlian Environmental Technology Co., Ltd., gifite imikorere myiza yo kwanduza ikirere hamwe na hydrogène peroxide yangiza, kandi irashobora kwica neza spore nizindi mikorobe.

1.4 Umwanya wikizamini: idirishya rifunze rya metero kibe 0.1.

2. Uburyo bwo kugerageza

Umubare w’imiti yica udukoko twashyizwe ku mashini yanduza ni 10ml, naho intumbero ni 100ml / m3, ikaba isumba cyane "GB27948-2020 Ibisabwa muri rusange byangiza imiti yangiza ikirere": iyo ukoresheje imiti yangiza imiti ya aerosol, ingano yanduza igomba kuba ≤10 ml / m3