Umubare muto wa A260 / A230 ubusanzwe uterwa numwanda ufite uburebure ntarengwa bwo kwinjiza kuri 230nm.Reka turebe icyo ibyo byanduye birimo:

• Umwanda rusange	Uburebure bwa Absorption	Ingaruka z'imibare
  Poroteyine	~ 230nm na 280nm	Kugabanuka icyarimwe A.260/A 280na A.260/A 280ibipimo
  Umunyu wa Guanidine	220-240 nm	Mugabanye A.260/A 280Ikigereranyo
  Fenol	~ 270nm	-
  Trizol	~ 230nm na 270nm	Mugabanye A.260/A 280Ikigereranyo
  EDTA	~ 230nm	Mugabanye A.260/A 280Ikigereranyo
  Ethanol	230-240 nm	Mugabanye A.260/A 280Ikigereranyo

 
 
 
Absorption yumurambararo nagereranya agaciro kanduye

Pkwanduza rotein
Umwanda wa poroteyine urashobora gufatwa nkumwanda ukabije mugikorwa cyo gukuramo aside nucleic.Poroteyine ibaho hagati yicyiciro cyo hejuru cyamazi yo hepfokamaicyiciro.Umwanda uzagabanya igipimo cya A260 / A280 na A260 / A230 icyarimwe, naho igipimo cya A260 / A230 kizahinduka cyane kuruta igipimo cya A260 / A280.
Mugihe cyakurikiyehoInyandikomvugoor QPCR, ibisigazwa bya poroteyine birashobora kubuza cyangwa kubangamira imikorere ya enzyme.Inzira nziza yo kwirinda kwanduza poroteyine ni ukuzirikana ihame rya "aho kuba munsi ya byinshi, umubare muto inshuro nyinshi" mugihe wifuza ndengakamere.

2. Umwanda wa Guanidinium
hydrochloride (GuHCl) na guanidine thiocyanate (GTC) bifite ingaruka zo gutandukanya poroteyine, zishobora kwangiza vuba uturemangingo mu gihe cyo gukuramo aside nucleic, bigatuma poroteyine ihindagurika ndetse n’imvura.Uburebure bwumurongo wa GuHCl na GTC buri hagati ya 220-240 nm, naumunyu wa guanidinium usigaye uzagabanya igipimo cya A260 / A230.Nubwo umunyu wa guanidini usigaye uzagabanya igipimo,Ingaruka kubigeragezo byo hasi mubyukuri ni ntangere.

3. Kwanduza Trizol
Ibice nyamukuru bigize Trizol ni phenol.Igikorwa nyamukuru cya fenol ni ugutera ingirabuzimafatizo no kurekura poroteyine hamwe na aside nucleic aside mu ngirabuzimafatizo.Nubwo fenol ishobora gutandukanya poroteyine neza, ntishobora kubuza rwose ibikorwa bya RNase.Kubwibyo, 8 -hydroxyquinoline, guanidine isothiocyanate, β- mercaptoethanol, nibindi byongewe kuri TRIzol kugirango bibuze RNase endogenous na exogenous RNase.
Iyo ikuramo RNA selile, Trizol irashobora guhita itera ingirabuzimafatizo kandi ikabuza nuclease isohoka mu ngirabuzimafatizo, kandi Trizol isigaye izagabanya cyane igipimo cya A260 / A230.
Uburyo bwo gutunganya: Iyo centrifuging, hagomba kumenyekana ko fenol muri Trizol ishonga byoroshye mugice cyamazi bitewe nubushyuhe bwa 4 ° nubushyuhe bwicyumba.

4. Ibisigisigi bya Ethanol
Ethanol ikoreshwa muburyo bwa nyuma kugirango igabanye ADN mugihe ushonga ion zumunyu zishobora guhuzwa na ADN.Kwinjira kwumurambararo muremureimpinga yaEthanol nayo iri kuri 230-240 nm, iyobizagabanya kandi igipimo cya A260 / A230.
Uburyo bwo kwirinda ibisigazwa bya Ethanol birashobora gusubirwamo kabiri mugihe cyo kurandura burundu, guhuha murifume hoodkuminota ibiri kugirango yemere Ethanol guhumeka neza mbere yo kongeramo buffer kugirango ikure.
Ariko, hakwiye kumenyekana ko igipimo ari igipimo cyo gusuzuma gusa ubuziranenge bwa RNA.Niba ibikorwa byavuzwe haruguru byateguwe neza, gutandukana hagati yikigereranyo nurwego rusanzwe ntabwo bizagira ingaruka zikomeye kubushakashatsi bwo hasi.
Ibicuruzwa bifitanye isano:
Amatungo yose ya RNA yigunga
Gutera Igiteranyo Cyuzuye RNA
Akagari Igiteranyo cya RNA Igikoresho cyo kwigunga
Tera Igiteranyo cya RNA Igikoresho cyongeyeho