Twizera: Guhanga udushya nubugingo bwacu numwuka.Ubuziranenge bwo hejuru ni ubuzima bwacu.Abaguzi bakeneye kugira Imana yacu kubuntu kubushakashatsi bwubushinwa Ubushishozi bukabije ADN / Rna Intambwe imwe Intambwe Rt-QpcrKit V2, Imbere muri gahunda zacu, dusanzwe dufite amaduka menshi mubushinwa kandi ibisubizo byacu byatsindiye ishimwe nabaguzi kwisi yose.Murakaza neza kubaguzi bashya kandi bataye igihe kugirango batwandikire kumurongo muremure wubucuruzi burambye.
Twizera: Guhanga udushya nubugingo bwacu numwuka.Ubuziranenge bwo hejuru ni ubuzima bwacu.Abaguzi bakeneye kugira Imana yacuUbushinwa Taq ADN Polymerase, Qpcr, Akazi gakomeye kugirango ukomeze gutera imbere, guhanga udushya mu nganda, kora ibishoboka byose kugirango umushinga wo mucyiciro cya mbere.Turagerageza uko dushoboye kugirango twubake uburyo bwo kuyobora siyanse, twige ubumenyi bwinshi bwumwuga, dutezimbere ibikoresho byumusaruro bigezweho hamwe nuburyo bwo kubyaza umusaruro, gukora ibicuruzwa byambere byita ibicuruzwa byiza, igiciro cyiza, serivisi nziza, gutanga byihuse, kugirango tuguhe agaciro gashya.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Byoroheje-icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
■ 2 × Kuvanga, kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
Preparation Imyiteguro yihuse-yuzuye irashobora kurangira muminota 10, PCR reaction irashobora kurangira byibuze muminota 50
■ Ibisubizo byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ibipimo by'ibikoresho

Gusaba: kumenyekanisha genetike, kumenyekanisha genotyping, nibindi.
Icyitegererezo: ikibabi cyibiti
Igipimo: Diameter 2-3mm (uburyo butaziguye), diameter 5-7mm (uburyo bwa lysis)
Urwego rwo gutahura: igice cyintego ≤1kb

Urujya n'uruza rw'akazi

Amashanyarazi

Ibyumweru bibiri bya PCR ivanga ibisubizo bivuye muburyo bwo kwanduza ibicuruzwa bya PCR.Twemera: Guhanga udushya nubugingo bwacu numwuka.Ubuziranenge bwo hejuru ni ubuzima bwacu.Abaguzi bakeneye kugira Imana yacu kubuntu kubuntu kubushinwa Ubushishozi bukabije ADN / Rna Intambwe imwe Probe Rt-Qpcr Kit V2, Imbere muri gahunda zacu, dusanzwe dufite amaduka menshi mubushinwa kandi ibisubizo byacu byatsindiye ishimwe kubaguzi kwisi yose.Murakaza neza kubaguzi bashya kandi bataye igihe kugirango batwandikire kumurongo muremure wubucuruzi burambye.
Icyitegererezo cyubusa kuriUbushinwa Taq ADN Polymerase, Qpcr, Akazi gakomeye kugirango ukomeze gutera imbere, guhanga udushya mu nganda, kora ibishoboka byose kugirango umushinga wo mucyiciro cya mbere.Turagerageza uko dushoboye kugirango twubake uburyo bwo kuyobora siyanse, twige ubumenyi bwinshi bwumwuga, dutezimbere ibikoresho byumusaruro bigezweho hamwe nuburyo bwo kubyaza umusaruro, gukora ibicuruzwa byambere byita ibicuruzwa byiza, igiciro cyiza, serivisi nziza, gutanga byihuse, kugirango tuguhe agaciro gashya.