Uruganda rukomoka mu Bushinwa Inkomoko ya Acide Nucleic Acide Sisitemu yo gukuramo ADN na Rna yoza no gukora ibicuruzwa |Foregene

Inkomoko y'uruganda Sisitemu yo gukuramo Acide Nucleic Acide ya ADN na Rna yoza

Inkomoko y'uruganda Sisitemu yo gukuramo Acide Nucleic Acide ya ADN na Rna Isukura Ikiranga

Ibisobanuro:

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

- Nta gutwara igihe kandi bihenze byo kweza ADN.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Sisitemu yo kurwanya umwanda PCR 2 × Imbuto PCR EasyTM ivanze (UNG), ishobora gukuraho neza umwanda uterwa n’ibicuruzwa bya PCR kandi ikemeza umwihariko n’ukuri kwa amplification.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Gushyigikirwa nitsinda ryitumanaho ryinzobere kandi ryinzobere, dushobora gutanga ubufasha bwa tekiniki kuri pre-sale & nyuma yo kugurisha isoko yuruganda Inkomoko ya Automatic Nucleic Acide Extraction Sisitemu ya ADN na Rna yo kweza, Niba hakenewe ibisobanuro birambuye, nyamuneka uduhamagare igihe icyo aricyo cyose!
Gushyigikirwa nitsinda ryitumanaho ryinzobere kandi ryinzobere, dushobora gutanga ubufasha bwa tekiniki kubicuruzwa mbere yo kugurisha & nyuma yo kugurisha kuri, Duharanira kuba indashyikirwa, guhora dutezimbere no guhanga udushya, twiyemeje kutugira "ikizere cyabakiriya" n "" guhitamo kwambere kwimashini zikoreshwa mubikoresho byububiko ".Hitamo, dusangire ibintu byunguka!

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice I.	Buffer P1
	Buffer P2
	6 Buffer Yipakurura ADN
Igice cya II	2 × Ibibabi PCR Byoroshye^TMKuvanga (UNG)

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Byoroheje-icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
■ 2 × Kuvanga, kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
Preparation Imyiteguro yihuse-yuzuye irashobora kurangira muminota 10, PCR reaction irashobora kurangira byibuze muminota 50
■ Ibisubizo byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ibipimo by'ibikoresho

Gusaba: kumenyekanisha genetike, kumenyekanisha genotyping, nibindi.
Icyitegererezo: ikibabi cyibiti
Igipimo: Diameter 2-3mm (uburyo butaziguye), diameter 5-7mm (uburyo bwa lysis)
Urwego rwo gutahura: igice cyintego ≤1kb

Urujya n'uruza rw'akazi

Gutera ibibabi bitaziguye PCR Kit-UNG4

Amashanyarazi

Gutera ibibabi bitaziguye PCR Kit-UNG5

Ibyumweru bibiri bya PCR ivanga ibisubizo byerekana imiterere ya PCR yongerewe ibicuruzwa.Kubera inkunga nitsinda ryambere ryinzobere kandi ryinzobere, dushobora gutanga ubufasha bwa tekiniki kubicuruzwa mbere yo kugurisha & nyuma yo kugurisha kubisoko biva mu ruganda Automatic Nucleic Acide Extraction Sisitemu ya ADN na Rna yo kweza, Niba bikenewe ibisobanuro birambuye, nyamuneka uduhamagare igihe icyo aricyo cyose!
Inkomoko y'uruganda, Duharanira kuba indashyikirwa, guhora dutezimbere no guhanga udushya, twiyemeje kutugira "ikizere cyabakiriya" n "" icyambere cyo guhitamo imashini zikoreshwa mubikoresho byububiko ".Hitamo, dusangire ibintu byunguka!

Mbere: Kugera gushya Ubushinwa Genomic ADN ikuramo ibikoresho (Amaraso / Amacandwe)

Ibikurikira: Icyiciro cya mbere cya virusi ya ADN na Rna Kwikuramo Gukuramo Kit Nucleic Acide Isukura Reagent ya PCR Igihe Cyukuri

Andika ubutumwa bwawe hano hanyuma utwohereze