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Kimwe Mubishyushye Mubushinwa Vtm Uruganda Rwagurisha Virusi Icyitegererezo Icyegeranyo Ikoreshwa rya Sampling Tube

Imwe Mubishyushye Mubushinwa Vtm Uruganda Rwagurisha Virusi Icyitegererezo Icyegeranyo Ikoreshwa rya Sampling Tube Ikiranga
  • Kimwe Mubishyushye Mubushinwa Vtm Uruganda Rwagurisha Virusi Icyitegererezo Icyegeranyo Ikoreshwa rya Sampling Tube
  • Kimwe Mubishyushye Mubushinwa Vtm Uruganda Rwagurisha Virusi Icyitegererezo Icyegeranyo Ikoreshwa rya Sampling Tube
  • Kimwe Mubishyushye Mubushinwa Vtm Uruganda Rwagurisha Virusi Icyitegererezo Icyegeranyo Ikoreshwa rya Sampling Tube

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN ya genomic kuva mubitangangingo byinyamanswa kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri lysis buffer cyarangiye muminota 10-30 kuri 65°C. Nta bundi buryo nka protein na RNA ikurwaho bisabwa, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2×PCR BiroroshyeTMKuvanga (UNG) ikoresha dUTP aho gukoresha dTTP hashingiwe kuri 2×PCR BiroroshyeTMKuvanga, hanyuma ukongeramo enzyme ya UNG (Uracil-N-glycosylase) ishobora gutesha agaciro inyandikorugero irimo dUTP icyarimwe.Mbere ya PCR reaction, enzyme ya UNG ikoreshwa mugutesha agaciro ibicuruzwa bya PCR birimo uracil.Enzyme ya UNG ntacyo izagira ku gishushanyo kitarimo uracil, bityo hakemezwa umwihariko nukuri kwokwiyongera no gukumira ko ibicuruzwa bya PCR byandura mugihe kinini cyo gupima geneti.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.


Ibicuruzwa birambuye

Ibicuruzwa

Intego yacu y'ibanze ni uguha abakiriya bacu umubano ukomeye kandi ufite inshingano zubucuruzi buciriritse, gutanga ibitekerezo byihariye kuri bose kuri imwe muri Hottest yo mu Bushinwa Vtm Uruganda Rwagurisha Virus Sample Collection Disposable Sampling Tube, Kugeza ubu, izina ryamasosiyete rifite ibicuruzwa birenga 4000 kandi byungutse cyane kandi bifite imigabane minini kumasoko agezweho mugihugu ndetse no mumahanga.
Intego yacu y'ibanze izaba iyo guha abakiriya bacu umubano muto kandi ufite inshingano zubucuruzi buto, gutanga ibitekerezo byihariye kuri boseUbushinwa Ikoreshwa rya Sampling Tube, Icyitegererezo cyo gukusanya Tube, Twakiriye neza amahirwe yo gukora ubucuruzi nawe kandi tunezezwa no guhuza amakuru arambuye kubicuruzwa byacu.Ubwiza buhebuje, ibiciro byapiganwa, gutanga igihe na serivisi yizewe birashobora kwizerwa.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.

Buffer AL

Foregene Protease

6 × ADN Yipakurura Buffer

Igice cya II

2 × PCR BiroroshyeTMKuvanga (UNG)

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN

Require Icyitegererezo gisabwa ni gito, nkibice 5mg byinyamanswa bishobora kugeragezwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

System Kurwanya umwanda PCR sisitemu 2 × PCR BiroroshyeTMKuvanga (UNG) bikuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: inyama zinyamaswa zitandukanye.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk flw-inyamanswa yinyamanswa PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

Reagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

Reagent 2 × PCR BiroroshyeTMKuvanga (UNG), niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kugirango ibike igihe gito (koresha mugihe cyiminsi 10) .Intego yacu yibanze izaba iyo guha abakiriya bacu umubano muto wubucuruzi muto kandi ufite inshingano, utanga ibitekerezo byihariye kuri bose kuri kimwe mubintu bishyushye kubushinwa Vtm Uruganda rukora ibicuruzwa bituruka kumasoko menshi kandi afite ibicuruzwa byinshi byinjira mu mahanga kandi bifite ibicuruzwa byinshi byinjira mu mahanga kandi bifite ibicuruzwa byinshi byinjira mu mahanga. .
Kimwe mu Bishyushye kuriUbushinwa Ikoreshwa rya Sampling Tube, Icyitegererezo cyo gukusanya Tube, Twakiriye neza amahirwe yo gukora ubucuruzi nawe kandi tunezezwa no guhuza amakuru arambuye kubicuruzwa byacu.Ubwiza buhebuje, ibiciro byapiganwa, gutanga igihe na serivisi yizewe birashobora kwizerwa.

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