Ubushinwa bwikora acide nucleic aside Gukora nuwabitanze |Foregene

gukuramo aside nucleic

Ibisobanuro:

Iki gikoresho gikoresha ikoranabuhanga rya Real-RT RT (rRT-PCR) kugirango hamenyekane neza SARS-CoV-2 (ORF1ab gene na N gene) nucleic acide muri nasofaryngeal cyangwa oropharyngeal swab sample, hakoreshejwe uburyo bwa multiplex PCR fluorescent probe.Iki gikoresho kirimo igenzura ryimbere kugirango risuzume ubwiza bwikigereranyo, bityo ntigikenewe kwipimisha nuclée nini,

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Dukurikiza amahame yubuyobozi bwa "Ubwiza ni budasanzwe, Utanga isoko ni hejuru, Izina ni irya mbere", kandi tuzarema tubikuye ku mutima kandi dusangire intsinzi hamwe nabakiriya bose kugirango bakuremo aside nucleique, twizera ko serivisi zacu zishyushye kandi zumwuga zizakuzanira ibintu bitangaje ndetse n'amahirwe.
Dukurikiza amahame yubuyobozi bwa "Ubwiza ni budasanzwe, Utanga ni hejuru, Izina ni irya mbere", kandi tuzarema tubikuye ku mutima kandi dusangire intsinzi nabakiriya bose kuriUbushinwa ADN / Rna Gukuramo Acide Nucleic, Sisitemu yo gukuramo aside Nucleic, Dufite uburambe buhagije mugukora ibicuruzwa ukurikije ingero cyangwa ibishushanyo.Twakiriye neza abakiriya baturutse mu gihugu ndetse no mu mahanga gusura isosiyete yacu, no gufatanya natwe ejo hazaza heza hamwe.

Ibisobanuro

48 Rxns, 96 Rxns

Oya.	Ibigize	Umubare				Ibice nyamukuru
Oya.	Ibigize	48 Rxns		96 Rxns		Ibice nyamukuru
1	Nucleic aside irekura	1.4mL / tube	Imiyoboro 2	5.3 mL / icupa	Icupa 1	Surfactant
2	Kurinda RNA	27 μL / umuyoboro	1 tube	53 μL / umuyoboro	1 tube	RNase inhibitor
3	SARS-CoV-2 igisubizo cyibisubizo	800 μL / umuyoboro	1 tube	1600 μL / umuyoboro	1 tube	Primer, probe, reaction reaction, dNTP
4	SARS-CoV-2 enzyme ivanze	80 μL / umuyoboro	1 tube	160 μL / umuyoboro	1 tube	Gutangira bishyushye Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 kugenzura neza	100 μL / umuyoboro	1 tube	100 μL / umuyoboro	1 tube	Recombinant plasmid ikubiyemo igice cyagenewe, RNA
6	SARS-CoV-2 kugenzura nabi	1200 μL / umuyoboro	1 tube	1200 μL / umuyoboro	1 tube	TE buffer

Ibiranga ibyiza

Byihuse kandi byoroshye.
Irinde gutakaza aside nucleic.
Umupaka muto wo gutahura no kwiyumvamo byinshi.
Ibikoresho bike bisabwa no gukoresha byoroshye.

Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).

GCE / mL	Ct agaciro
GCE / mL	N gen	ORF1ab
2.00E + 08	16.43	15.16
2.00E + 07	19.48	18.58
2.00E + 06	22.71	21.86
2.00E + 05	25.95	25.33
2.00E + 04	29.30	28.49
2.00E + 03	32.37	31.37
2.00E + 02	35.32	34.47
2.00E + 01	38.29	38.27
2.00E + 00	N / A.	N / A.
2.00E-01	N / A.	N / A.
NC	N / A.	N / A.

Umurongo wo kwongera umurongo

amplification curve-RT-qPCR

Uburyo bwo gukoresha

1. Igikoresho gikoreshwa

covid-19 nucleic aside itahura kit1

2. Uburyo bwo gutahura

covid-19 nucleic aside itahura kit2

Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (gukora intambwe 1 gusa) (Tegereza iminota 10)

PCR amplificaton (<= 55min)

Urupapuro rw'akazi:

covid-19 nucleic aside itahura kit4

Ububiko

bifunze ku mucyo kandi bibitswe kuri -20 ± 5 ℃;

ububiko
ububiko2

Ubuzima bwa Shelf:Umwaka 1

Dukurikiza amahame yubuyobozi bwa "Ubwiza ni budasanzwe, Utanga isoko ni hejuru, Izina ni ryo rya mbere", kandi tuzarema tubikuye ku mutima kandi dusangire intsinzi hamwe n’abakiriya bose ba PriceList yo mu Bushinwa Biobase PCR Laboratoire ADN / Rna Nucleic Acide Extraction (Betsy), Twizera ko serivisi zacu zishyushye kandi zumwuga zizakuzanira ibintu bitangaje ndetse n'amahirwe.
UrutondeUbushinwa ADN / Rna Gukuramo Acide Nucleic, Sisitemu yo gukuramo aside Nucleic, Dufite uburambe buhagije mugukora ibicuruzwa ukurikije ingero cyangwa ibishushanyo.Twakiriye neza abakiriya baturutse mu gihugu ndetse no mu mahanga gusura isosiyete yacu, no gufatanya natwe ejo hazaza heza hamwe.

Mbere: 2019 Ubushinwa Bwashushanyije Ubushinwa Bwimurwa Igihe Cyukuri PCR Kumenyekanisha Sisitemu Yahujwe na Nucleic Acide Ikuramo Automatic PCR Rna Kwagura

Ibikurikira: Kugabanuka cyane Ubushinwa PCR Ikizamini Reagent Igihe Cyukuri Ubuvuzi Gusuzuma Nucleic Acide Ikizamini

Andika ubutumwa bwawe hano hanyuma utwohereze