Isoko ryinshi rya ODM Ubushinwa Ukoresheje Peltier-ishingiye kuri Thermal Cycler PCR
Uyu muryango ukomeza inzira yuburyo bukoreshwa "ubuyobozi bwa siyanse, ubuziranenge buhebuje kandi bukora neza, umuguzi w'ikirenga kuri ODM Ubushinwa Bwinshi bukoresha Peltier-BishingiyeUmukinnyi w'amagarePCR, Niba bikenewe, ikaze kugirango utumenyeshe kurupapuro rwurubuga cyangwa kugisha inama kuri terefone, tuzishimira kugukorera.
Ishyirahamwe rikomeza inzira yuburyo "ubuyobozi bwa siyanse, ubuziranenge buhebuje kandi bukora neza, umuguzi arenze kuriUbushinwa PCR, Umukinnyi w'amagare, Bitewe nimpinduka zigenda zihinduka muriki gice, twishora mubucuruzi bwibicuruzwa hamwe nimbaraga zabigenewe hamwe nubuyobozi bwiza.Tugumana gahunda yo gutanga ku gihe, ibishushanyo mbonera, ubwiza no gukorera mu mucyo kubakiriya bacu.Moto yacu ni ugutanga ibisubizo byiza mugihe giteganijwe.
Ibisobanuro
48 Rxns, 96 Rxns
Oya. | Ibigize | Umubare | Ibice nyamukuru | |||
48 Rxns | 96 Rxns | |||||
1 | Nucleic aside irekura | 1.4mL / tube | Imiyoboro 2 | 5.3 mL / icupa | Icupa 1 | Surfactant |
2 | Kurinda RNA | 27 μL / umuyoboro | 1 tube | 53 μL / umuyoboro | 1 tube | RNase inhibitor |
3 | SARS-CoV-2 igisubizo cyibisubizo | 800 μL / umuyoboro | 1 tube | 1600 μL / umuyoboro | 1 tube | Primer, probe, reaction reaction, dNTP |
4 | SARS-CoV-2 enzyme ivanze | 80 μL / umuyoboro | 1 tube | 160 μL / umuyoboro | 1 tube | Gutangira bishyushye Taq enzyme, M-MLV enzyme |
5 | SARS-CoV-2 kugenzura neza | 100 μL / umuyoboro | 1 tube | 100 μL / umuyoboro | 1 tube | Recombinant plasmid ikubiyemo igice cyagenewe, RNA |
6 | SARS-CoV-2 kugenzura nabi | 1200 μL / umuyoboro | 1 tube | 1200 μL / umuyoboro | 1 tube | TE buffer |
Ibiranga ibyiza
Byihuse kandi byoroshye.
Irinde gutakaza aside nucleic.
Umupaka muto wo gutahura no kwiyumvamo byinshi.
Ibikoresho bike bisabwa no gukoresha byoroshye.
Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).
GCE / mL | Ct agaciro | |
N gen | ORF1ab | |
2.00E + 08 | 16.43 | 15.16 |
2.00E + 07 | 19.48 | 18.58 |
2.00E + 06 | 22.71 | 21.86 |
2.00E + 05 | 25.95 | 25.33 |
2.00E + 04 | 29.30 | 28.49 |
2.00E + 03 | 32.37 | 31.37 |
2.00E + 02 | 35.32 | 34.47 |
2.00E + 01 | 38.29 | 38.27 |
2.00E + 00 | N / A. | N / A. |
2.00E-01 | N / A. | N / A. |
NC | N / A. | N / A. |
Umurongo wo kwongera umurongo
Uburyo bwo gukoresha
1. Igikoresho gikoreshwa
2. Uburyo bwo gutahura
Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (gukora intambwe 1 gusa) (Tegereza iminota 10)
PCR amplificaton (<= 55min)
Urupapuro rw'akazi:
Ububiko
bifunze ku mucyo kandi bibitswe kuri -20 ± 5 ℃;
Ubuzima bwa Shelf:Umwaka 1Umuryango ukomeza inzira yuburyo bukoreshwa "ubuyobozi bwa siyanse, ubuziranenge buhebuje kandi bukora neza, umuguzi w'ikirenga kuri Laboratwari ya ODM y'Ubushinwa ukoresheje Peltier-ishingiye kuri Thermal Cycler PCR, Niba bikenewe, urakaza neza kugirango utumenyeshe kurubuga rwacu cyangwa kugisha inama kuri terefone, tuzishimira kugukorera.
ODMUbushinwa PCR, Amagare yubushyuhe, Kubera impinduka zigenda zihinduka muriki gice, twishora mubucuruzi bwibicuruzwa n'imbaraga zabigenewe hamwe nubuyobozi bwiza.Tugumana gahunda yo gutanga ku gihe, ibishushanyo mbonera, ubwiza no gukorera mu mucyo kubakiriya bacu.Moto yacu ni ugutanga ibisubizo byiza mugihe giteganijwe.