Dutsimbaraye ku myumvire ya "Gukora ibicuruzwa byujuje ubuziranenge no kugirana ubucuti bwiza n'abantu muri iki gihe baturutse impande zose z'isi", duhora dushiraho inyungu z'abaguzi gutangirana na 2019 Ibikoresho byo mu rwego rwo hejuru byo mu Bushinwa ibikoresho byo mu bwoko bwa Portable Real-Time PCR Sisitemu ADN / Rna Extraction Kit, Tugiye guha imbaraga abantu tuvugana kandi twumva, Dutanga urugero kubandi kandi twigire kuburambe.
Dutsimbaraye ku myumvire ya "Gukora ibicuruzwa byujuje ubuziranenge no kugirana ubucuti bwiza n'abantu muri iki gihe baturutse impande zose z'isi", duhora dushiraho inyungu z'abaguzi gutangirira kuriUbushinwa Gukuramo Acide Nucleic, Gukuramo Acide Nucleic, Turashimangira kuri "Ubwiza Bwambere, Icyubahiro Mbere na mbere Umukiriya".Twiyemeje gutanga ibicuruzwa byiza kandi byiza nyuma yo kugurisha.Kugeza ubu, ibicuruzwa byacu byoherejwe mu bihugu birenga 60 byo ku isi, nka Amerika, Ositaraliya n'Uburayi.Twishimiye cyane mu gihugu no hanze yacyo.Buri gihe gutsimbarara ku ihame rya "Inguzanyo, Umukiriya n'Ubuziranenge", turateganya ubufatanye n'abantu b'ingeri zose kubwinyungu zabo.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yujuje ubuziranenge RNA yose mubice bitandukanye byibimera bifite polysaccharide nkeya hamwe na polifenol.Kubitegererezo byibihingwa bifite polysaccharide nyinshi cyangwa polifenole, birasabwa gukoresha Plant Total RNA Isolation Plus Kit kugirango ubone ibisubizo byiza byo gukuramo RNA.Igikoresho gitanga ADN-Isukura inkingi ishobora gukuraho ADN genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nkeya hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) ahantu humye, na 2-8 ℃ mugihe kirekire (amezi 24).

Buffer PSL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-ethanethiol (itabishaka) .Gukurikiza imyumvire ya "Gukora ibicuruzwa bifite ireme kandi ugirana ubucuti bwiza nabantu muri iki gihe ku isi yose", duhora dushiraho inyungu zabaguzi gutangirana na 2019 Ibikoresho byiza byubuvuzi byu Bushinwa Bikwirakwizwa no kwimenyereza abantu hamwe na hamwe.
2019 Ubwiza buhanitseUbushinwa Gukuramo Acide Nucleic, Gukuramo Acide Nucleic, Turashimangira kuri "Ubwiza Bwambere, Icyubahiro Mbere na mbere Umukiriya".Twiyemeje gutanga ibicuruzwa byiza kandi byiza nyuma yo kugurisha.Kugeza ubu, ibicuruzwa byacu byoherejwe mu bihugu birenga 60 byo ku isi, nka Amerika, Ositaraliya n'Uburayi.Twishimiye cyane mu gihugu no hanze yacyo.Buri gihe gutsimbarara ku ihame rya "Inguzanyo, Umukiriya n'Ubuziranenge", turateganya ubufatanye n'abantu b'ingeri zose kubwinyungu zabo.