Kugira ngo duhuze ibyifuzo byabakiriya byateganijwe, dufite abakozi bacu bakomeye kugirango batange inkunga nziza cyane zirimo kwamamaza, kwinjiza, kuzana, umusaruro, gucunga neza, gupakira, kubika no kubika ibikoresho bishya bishyushye Ubushinwa Plastike Magnetic Bead Method Universal Nucleic Acide Extraction Rapid Kit, Kugeza ubu, turategereje ubufatanye bunini n’abakiriya bo mu mahanga dushingiye ku nyungu rusange.Menya neza ko uza kumva nta kiguzi cyo gukora natwe kugirango tumenye amakuru menshi.
Kugirango duhuze ibyifuzo byabakiriya byateganijwe, dufite abakozi bacu bakomeye kugirango batange inkunga nziza cyane zirimo kwamamaza, kwinjiza, kuzana, umusaruro, gucunga neza, gupakira, kubika no kubika ibikoreshoUbushinwa Gukuramo Acide Nucleic Universal, Gukuramo Acide ya Nucleic Acide, Noneho amarushanwa muriki gice arakaze cyane;ariko tuzakomeza gutanga ubuziranenge bwiza, buringaniye hamwe na serivisi zitaweho cyane kugirango tugere ku ntego yo gutsinda.“Hindura ibyiza!”ni intero yacu, isobanura ngo "Isi nziza iri imbere yacu, reka rero tuyishimire!"Hindura ibyiza!Uriteguye?

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yujuje ubuziranenge RNA yose mubice bitandukanye byibimera bifite polysaccharide nkeya hamwe na polifenol.Kubitegererezo byibihingwa bifite polysaccharide nyinshi cyangwa polifenole, birasabwa gukoresha Plant Total RNA Isolation Plus Kit kugirango ubone ibisubizo byiza byo gukuramo RNA.Igikoresho gitanga ADN-Isukura inkingi ishobora gukuraho ADN genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nkeya hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) ahantu humye, na 2-8 ℃ mugihe kirekire (amezi 24).

Buffer PSL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka) .Kugirango duhuze ibyifuzo byabakiriya byateganijwe birenze urugero, dufite abakozi bacu bakomeye kugirango batange inkunga nziza cyane zirimo ibicuruzwa, kwinjiza, kuzana, umusaruro, gucunga neza, gupakira, kubika no kubika ibikoresho bishyushye bikoreshwa mububiko bwa Plastike Magnetic Bead Method abakiriya bo mu mahanga bashingiye ku nyungu rusange.Menya neza ko uza kumva nta kiguzi cyo gukora natwe kugirango tumenye amakuru menshi.
Ibicuruzwa bishya bishyushyeUbushinwa Gukuramo Acide Nucleic Universal, Gukuramo Acide ya Nucleic Acide, Noneho amarushanwa muriki gice arakaze cyane;ariko tuzakomeza gutanga ubuziranenge bwiza, buringaniye hamwe na serivisi zitaweho cyane kugirango tugere ku ntego yo gutsinda.“Hindura ibyiza!”ni intero yacu, isobanura ngo "Isi nziza iri imbere yacu, reka rero tuyishimire!"Hindura ibyiza!Uriteguye?