Ubushinwa Igiciro cyiza kubushinwa Igihe nyacyo Fluorescence Virus Nucleic Acide PCR Ikizamini Cyakozwe nuwitanga |Foregene

Igiciro cyiza kubushinwa Igihe nyacyo Fluorescence Virus Nucleic Acide PCR Ikizamini

Igiciro cyumvikana kubushinwa Igihe nyacyo Fluorescence Virus Nucleic Acide PCR Ikizamini cyerekanwe

Ibisobanuro:

Iki gikoresho gikoresha ikoranabuhanga rya Real-RT RT (rRT-PCR) kugirango hamenyekane neza SARS-CoV-2 (ORF1ab gene na N gene) nucleic acide muri nasofaryngeal cyangwa oropharyngeal swab sample, hakoreshejwe uburyo bwa multiplex PCR fluorescent probe.Iki gikoresho kirimo igenzura ryimbere kugirango risuzume ubwiza bwikigereranyo, bityo ntigikenewe kwipimisha nuclée nini,

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Abakozi bacu bakunze kuba mugihe cyo "gukomeza gutera imbere no kuba indashyikirwa", kandi tugakoresha ibintu byiza bidasanzwe, igiciro cyiza nibicuruzwa byiza na nyuma yo kugurisha, turagerageza kubona hafi imyizerere ya buri mukiriya kubiciro bifatika kubushinwa Real Time Fluorescence Virus Nucleic Acide PCR Ikizamini, Kubindi bisobanuro ugomba kumenya neza ko udategereza ko dukorana natwe.Urakoze - Imfashanyo yawe idutera imbaraga.
Abakozi bacu bakunze kuba mugihe cyo "gukomeza gutera imbere no kuba indashyikirwa", kandi dukoresha ibintu byiza bidasanzwe, igiciro cyiza nibicuruzwa na serivisi nyuma yo kugurisha, turagerageza kubona hafi imyizerere ya buri mukiriya kuriUbushinwa PCR Ikizamini, Ikizamini cyo Gusuzuma Byihuse, Intego yacu ni ugufasha abakiriya kumenya intego zabo.Twakomeje gukora ibishoboka byose kugirango tugere kuri iki kibazo cyo gutsindira inyungu kandi turabashimira byimazeyo ko twifatanya natwe.Mu ijambo, iyo uduhisemo, uhitamo ubuzima butunganye.Murakaza neza gusura uruganda rwacu kandi mwakire neza ibyo mwategetse!Kubindi bisobanuro, ibuka kudatindiganya kutwandikira.

Ibisobanuro

48 Rxns, 96 Rxns

Oya.	Ibigize	Umubare				Ibice nyamukuru
Oya.	Ibigize	48 Rxns		96 Rxns		Ibice nyamukuru
1	Nucleic aside irekura	1.4mL / tube	Imiyoboro 2	5.3 mL / icupa	Icupa 1	Surfactant
2	Kurinda RNA	27 μL / umuyoboro	1 tube	53 μL / umuyoboro	1 tube	RNase inhibitor
3	SARS-CoV-2 igisubizo cyibisubizo	800 μL / umuyoboro	1 tube	1600 μL / umuyoboro	1 tube	Primer, probe, reaction reaction, dNTP
4	SARS-CoV-2 enzyme ivanze	80 μL / umuyoboro	1 tube	160 μL / umuyoboro	1 tube	Gutangira bishyushye Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 kugenzura neza	100 μL / umuyoboro	1 tube	100 μL / umuyoboro	1 tube	Recombinant plasmid ikubiyemo igice cyagenewe, RNA
6	SARS-CoV-2 kugenzura nabi	1200 μL / umuyoboro	1 tube	1200 μL / umuyoboro	1 tube	TE buffer

Ibiranga ibyiza

Byihuse kandi byoroshye.
Irinde gutakaza aside nucleic.
Umupaka muto wo gutahura no kwiyumvamo byinshi.
Ibikoresho bike bisabwa no gukoresha byoroshye.

Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).

GCE / mL	Ct agaciro
GCE / mL	N gen	ORF1ab
2.00E + 08	16.43	15.16
2.00E + 07	19.48	18.58
2.00E + 06	22.71	21.86
2.00E + 05	25.95	25.33
2.00E + 04	29.30	28.49
2.00E + 03	32.37	31.37
2.00E + 02	35.32	34.47
2.00E + 01	38.29	38.27
2.00E + 00	N / A.	N / A.
2.00E-01	N / A.	N / A.
NC	N / A.	N / A.

Umurongo wo kwongera umurongo

amplification curve-RT-qPCR

Uburyo bwo gukoresha

1. Igikoresho gikoreshwa

covid-19 nucleic aside itahura kit1

2. Uburyo bwo gutahura

covid-19 nucleic aside itahura kit2

Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (gukora intambwe 1 gusa) (Tegereza iminota 10)

PCR amplificaton (<= 55min)

Urupapuro rw'akazi:

covid-19 nucleic aside itahura kit4

Ububiko

bifunze ku mucyo kandi bibitswe kuri -20 ± 5 ℃;

ububiko
ububiko2

Ubuzima bwa Shelf:Umwaka 1

Abakozi bacu bakunze kuba mugihe cyo "gukomeza gutera imbere no kuba indashyikirwa", kandi tugakoresha ibintu byiza bidasanzwe, igiciro cyiza nibicuruzwa byiza na nyuma yo kugurisha, turagerageza kubona hafi imyizerere ya buri mukiriya kubiciro bifatika kubushinwa Real Time Fluorescence Virus Nucleic Acide PCR Ikizamini, Kubindi bisobanuro ugomba kumenya neza ko udategereza ko dukorana natwe.Urakoze - Imfashanyo yawe idutera imbaraga.
Igiciro cyumvikana kuriUbushinwa PCR Ikizamini, Ikizamini cyo Gusuzuma Byihuse, Intego yacu ni ugufasha abakiriya kumenya intego zabo.Twakomeje gukora ibishoboka byose kugirango tugere kuri iki kibazo cyo gutsindira inyungu kandi turabashimira byimazeyo ko twifatanya natwe.Mu ijambo, iyo uduhisemo, uhitamo ubuzima butunganye.Murakaza neza gusura uruganda rwacu kandi mwakire neza ibyo mwategetse!Kubindi bisobanuro, ibuka kudatindiganya kutwandikira.

Mbere: Ibisobanuro bihanitse Ubushinwa Gukuramo Acide Nucleic Acide Yc-701 Cov Nucleic Acide

Ibikurikira: Quots kubushinwa Byuzuye Automatic Reagents Extract Nucleic Acide (Generotex)

Andika ubutumwa bwawe hano hanyuma utwohereze