Ubushinwa Ibisobanuro bihanitse Ubushinwa Gukuramo Acide Nucleic Gukuramo Yc-701 Cov Nucleic Acide Nukora nuwabitanga |Foregene

Ibisobanuro bihanitse Ubushinwa Gukuramo Acide Nucleic Acide Yc-701 Cov Nucleic Acide

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN ya genomic kuva mubitangangingo byinyamanswa kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri lysis buffer cyarangiye muminota 10-30 kuri 65°C. Nta bundi buryo nka protein na RNA ikurwaho bisabwa, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2×PCR Biroroshye^TMKuvanga (UNG) ikoresha dUTP aho gukoresha dTTP hashingiwe kuri 2×PCR Biroroshye^TMKuvanga, hanyuma ukongeramo enzyme ya UNG (Uracil-N-glycosylase) ishobora gutesha agaciro inyandikorugero irimo dUTP icyarimwe.Mbere ya PCR reaction, enzyme ya UNG ikoreshwa mugutesha agaciro ibicuruzwa bya PCR birimo uracil.Enzyme ya UNG ntacyo izagira ku gishushanyo kitarimo uracil, bityo hakemezwa umwihariko nukuri kwokwiyongera no gukumira ko ibicuruzwa bya PCR byandura mugihe kinini cyo gupima geneti.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Twumiye ku ihame ry "ubuziranenge bwa mbere, serivisi mbere, gukomeza kunoza no guhanga udushya kugira ngo duhure n’abakiriya" kubuyobozi na "inenge zeru, ibibazo bya zeru" nkintego nziza.Kugira ngo serivisi zacu zirusheho kugenda neza, dutanga ibicuruzwa bifite ireme ryiza ku giciro cyiza kubisobanuro bihanitse Ubushinwa Nucleic Acide Yogukuramo Acide Yc-701 Cov Nucleic Acide, Kwibanda cyane kubipfunyika byibicuruzwa kugirango twirinde ibyangiritse mugihe cyo gutwara, Inyungu zirambuye kubitekerezo byingirakamaro hamwe ningamba zabaguzi bacu bubahwa.
Twumiye ku ihame ry "ubuziranenge bwa mbere, serivisi mbere, gukomeza kunoza no guhanga udushya kugira ngo duhure n’abakiriya" kubuyobozi na "inenge zeru, ibibazo bya zeru" nkintego nziza.Kugirango tunoze serivisi zacu, dutanga ibicuruzwa bifite ireme ryiza kubiciro byizaSisitemu yo gukuramo aside Nucleic, Igikoresho cyo gukuramo aside Nucleic, Turimo kwagura umugabane mpuzamahanga ku isoko dushingiye ku bintu byiza, serivisi nziza, igiciro cyiza no gutanga ku gihe.Nyamuneka twandikire igihe icyo aricyo cyose kugirango ubone ibisobanuro byinshi.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer AL
	Foregene Protease
	6 × ADN Yipakurura Buffer
Igice cya II	2 × PCR Biroroshye^TMKuvanga (UNG)

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN

Require Icyitegererezo gisabwa ni gito, nkibice 5mg byinyamanswa bishobora kugeragezwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

System Kurwanya umwanda PCR sisitemu 2 × PCR Biroroshye^TMKuvanga (UNG) bikuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: inyama zinyamaswa zitandukanye.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk flw-inyamanswa yinyamanswa PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

Reagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

Reagent 2 × PCR Biroroshye^TMKuvanga (UNG), niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kugirango ibike igihe gito (koresha mugihe cyiminsi 10) .Twubahiriza ihame ry "ubuziranenge bwambere, serivisi mbere, guhora utezimbere no guhanga udushya kugirango duhure nabakiriya" kubuyobozi na "zero inenge, ibirego bya zeru" nkintego yubuziranenge.Kugira ngo serivisi zacu zirusheho kugenda neza, dutanga ibicuruzwa bifite ireme ryiza ku giciro cyiza kubisobanuro bihanitse Ubushinwa Nucleic Acide Yogukuramo Acide Yc-701 Cov Nucleic Acide, Kwibanda cyane kubipfunyika byibicuruzwa kugirango twirinde ibyangiritse mugihe cyo gutwara, Inyungu zirambuye kubitekerezo byingirakamaro hamwe ningamba zabaguzi bacu bubahwa.
Ibisobanuro bihanitseSisitemu yo gukuramo aside Nucleic, Igikoresho cyo gukuramo aside Nucleic, Turimo kwagura umugabane mpuzamahanga ku isoko dushingiye ku bintu byiza, serivisi nziza, igiciro cyiza no gutanga ku gihe.Nyamuneka twandikire igihe icyo aricyo cyose kugirango ubone ibisobanuro byinshi.

Mbere: Isonga ryo mu Bushinwa Virus Nucleic Acide Ikuramo Kit, Nucleic Acide Ikizamini Agasanduku, Acide Nucleic

Ibikurikira: Igiciro cyiza kubushinwa Igihe nyacyo Fluorescence Virus Nucleic Acide PCR Ikizamini

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