Intego yacu izaba iyo kuzuza abaguzi bacu batanga ubufasha bwa zahabu, igiciro cyiza kandi cyiza cyo hejuru kubushinwa Bwiza 96 Ikibaho Cyiza cya ADN / Rna Nucleic Acide Extract Isolation Reagent Kit hamwe na Magnetic Bead Method yo kumenya PCR, Ubu dufite itsinda ryabahanga mubucuruzi mpuzamahanga.Tuzakemura ikibazo muhuye.Turashobora kwerekana ibicuruzwa ushaka.Witondere kumva nta kiguzi watwandikira.
Intego yacu izaba iyo kuzuza abaguzi bacu batanga ubufasha bwa zahabu, igiciro cyiza kandi cyiza kuriUbushinwa ADN Rna Kwigunga, Virus Nucleic Acide Isolation, Ako kanya ninzobere nyuma yo kugurisha itangwa nitsinda ryacu ryabajyanama ryishimiye abaguzi bacu.Amakuru arambuye hamwe nibipimo bivuye mubicuruzwa birashoboka koherezwa kubwawe kubyemera byuzuye.Ingero z'ubuntu zirashobora gutangwa kandi isosiyete ikagenzura isosiyete yacu.n Maroc kugirango imishyikirano ihora ikaze.Twizere kubona anketi wandike kandi wubake ubufatanye bwigihe kirekire.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Intego yacu izaba iyo kuzuza abaguzi bacu batanga ubufasha bwa zahabu, igiciro cyiza kandi cyiza cyo hejuru kubushinwa Bwiza 96 Ikibaho Cyiza cya ADN / Rna Nucleic Acide Extract Isolation Reagent Kit hamwe na Magnetic Bead Method yo kumenya PCR, Ubu dufite itsinda ryabahanga mubucuruzi mpuzamahanga.Tuzakemura ikibazo muhuye.Turashobora kwerekana ibicuruzwa ushaka.Witondere kumva nta kiguzi watwandikira.
Ubuziranenge bwo hejuruUbushinwa ADN Rna Kwigunga, Virus Nucleic Acide Isolation, Ako kanya ninzobere nyuma yo kugurisha itangwa nitsinda ryacu ryabajyanama ryishimiye abaguzi bacu.Amakuru arambuye hamwe nibipimo bivuye mubicuruzwa birashoboka koherezwa kubwawe kubyemera byuzuye.Ingero z'ubuntu zirashobora gutangwa kandi isosiyete ikagenzura isosiyete yacu.n Maroc kugirango imishyikirano ihora ikaze.Twizere kubona anketi wandike kandi wubake ubufatanye bwigihe kirekire.