Yubahiriza amahame yawe "Kuba inyangamugayo, umunyamwete, kwihangira imirimo, guhanga udushya" kugirango utange ibisubizo bishya buri gihe.Ireba abaguzi, intsinzi nkitsinzi yayo.Reka dutezimbere ejo hazaza heza mu ntoki kubushinwa bushya Longyu Gutanga Ubwiza Bwiza Bwiza Bwiza bwa RNA, Twishimiye abakiriya bose ninshuti kutwandikira kugirango tubone inyungu.Twizere ko uzakora ubucuruzi hamwe nawe.
Yubahiriza amahame yawe "Kuba inyangamugayo, umunyamwete, kwihangira imirimo, guhanga udushya" kugirango utange ibisubizo bishya buri gihe.Ireba abaguzi, intsinzi nkitsinzi yayo.Reka dutezimbere ejo hazaza heza mu ntokiUbushinwa Bwiza RNA nziza, Gukuramo ibisubizo, Intego yacu ni ugufasha abakiriya kumenya intego zabo.Turimo gukora ibishoboka byose kugirango tugere kuri iki kibazo cyo gutsindira inyungu kandi turakwishimiye rwose ko uza kwifatanya natwe.Mu ijambo, iyo uduhisemo, uhitamo ubuzima butunganye.Murakaza neza gusura uruganda rwacu kandi mwakire neza ibyo mwategetse!Kubindi bisobanuro, nyamuneka ntutindiganye kutwandikira.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Yubahiriza amahame yawe "Kuba inyangamugayo, umunyamwete, kwihangira imirimo, guhanga udushya" kugirango utange ibisubizo bishya buri gihe.Ireba abaguzi, intsinzi nkitsinzi yayo.Reka dutezimbere ejo hazaza heza muntoki kubushinwa bushya Longyu Gutanga imbuto Yizewe Yimbuto Yimbuto Yimbuto, Twishimiye abakiriya bose ninshuti kutwandikira kugirango tubone inyungu.Twizere ko uzakora ubucuruzi hamwe nawe.
Kugera Gishya Ubushinwa Gukuramo Imbuto Zimbuto,Gukuramo ibisubizo, Intego yacu ni ugufasha abakiriya kumenya intego zabo.Turimo gukora ibishoboka byose kugirango tugere kuri iki kibazo cyo gutsindira inyungu kandi turakwishimiye rwose ko uza kwifatanya natwe.Mu ijambo, iyo uduhisemo, uhitamo ubuzima butunganye.Murakaza neza gusura uruganda rwacu kandi mwakire neza ibyo mwategetse!Kubindi bisobanuro, nyamuneka ntutindiganye kutwandikira.