Ubushinwa buyobora PCR kit Inganda nuwitanga |Foregene

ibikoresho bya PCR

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN genomic muri zebrafish nizindi nyama zamafi yamazi meza, imirizo yumurizo cyangwa amagi y amafi byerekana PCR, bityo rero birakwiriye cyane cyane kwipimisha geneti nini.Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × PCR EasyTM Ivanga ifite kwihanganira bikomeye PCR ibuza gukora, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, buffer reaction, optimizer ya PCR na stabilisateur.Ikoreshwa ifatanije na lysis buffer irashobora kubona byihuse kandi byoroshye ingero, kandi ifite ibiranga sensibilité yo hejuru, umwihariko ukomeye, hamwe no guhagarara neza.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Buri gihe dushyira mu bikorwa umwuka wacu wa "Guhanga udushya kuzana iterambere, Ubwiza buhebuje butuma umuntu abaho neza, Ubuyobozi bwamamaza hamwe ninyungu zo kwamamaza, amanota yinguzanyo akurura abaguzi kubikoresho bya PCR bitaziguye, Twakomeje gushakisha imbere kugirango dufatanye ibyifuzo byose biva mu gihugu ndetse no hanze yarwo.Byongeye kandi, kuzuza abakiriya nibyo dukurikirana ubuziraherezo.
Buri gihe dusohoza umwuka wacu wa "Guhanga udushya tuzana iterambere, Ubwiza-bwiza bwo kubaho neza, Kwamamaza imiyoborere ninyungu zo kwamamaza, amanota yinguzanyo akurura abaguzi kuriPcr Kit, Twategereje gufatanya nawe hafi kubwinyungu zacu niterambere ryambere.Twijeje ubuziranenge, niba abakiriya batanyuzwe nubwiza bwibicuruzwa, urashobora kugaruka muminsi 7 hamwe na leta zabo zambere.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer FP
	Foregene Protease
	6 × ADN Yipakurura Buffer
Igice cya II	2 × PCR Biroroshye^TMKuvanga
Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Amafi ya Zebra nandi mafi yamazi meza.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk-flw-zebra-ifi-itaziguye-PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer FP reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

v Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

vReagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

vReagent 2 × PCR EasyTM ivanze, niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).

Buri gihe dushyira mu bikorwa umwuka wacu wa "Guhanga udushya kuzana iterambere, Ubwiza buhebuje butuma umuntu abaho neza, Ubuyobozi bwo kwamamaza no kunguka ibicuruzwa, amanota y'inguzanyo akurura abaguzi kuri Big Discount China Cdv AG The Canine Distemper Virus Antigen Rapid Test Kit, Twakomeje gushakisha imbere kugirango dufatanye ibyifuzo byose biva mu gihugu ndetse no mumahanga.Byongeye kandi, kuzuza abakiriya nibyo dukurikirana ubuziraherezo.
Ikarita nini yo kugabanura Ubushinwa Ikarita y'Ibizamini, Ibipimo by'ibizamini, Twategerezanyije amatsiko gufatanya nawe mu nyungu zacu ndetse no kwiteza imbere.Twijeje ubuziranenge, niba abakiriya batanyuzwe nubwiza bwibicuruzwa, urashobora kugaruka muminsi 7 hamwe na leta zabo zambere.

Mbere: Ubwiza buhebuje / ubunyangamugayo ibikoresho byose bya RNA byo kwigunga

Ibikurikira: Uruganda rutanga mu buryo butaziguye Ubushinwa Amapine Yambere Yabagenzi Amapine PCR Traire 155r12 185r14c 195r14c 205 / 55r16 215 / 45r17

Andika ubutumwa bwawe hano hanyuma utwohereze