Dushikamye ku mahame shingiro ya "Ubwiza bushobora kuba ubuzima bwumuryango wawe, kandi izina rishobora kuba ubugingo bwaryo" kurigukuramo RNA yose, Urakoze gufata umwanya wawe ukwiye wo kutugana kandi ugakomeza kugira ubufatanye bwiza hamwe nawe.
Dushikamye ku mahame shingiro ya "Ubwiza bushobora kuba ubuzima bwumuryango wawe, kandi izina rishobora kuba ubugingo bwaryo" kurigukuramo RNA yose, Isosiyete yacu isezeranya: ibiciro byumvikana, igihe gito cyo gukora na serivisi ishimishije nyuma yo kugurisha, turakwemera kandi gusura uruganda rwacu igihe cyose ubishakiye.Twifurije kubona ubucuruzi bushimishije kandi burebure hamwe !!!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Uruganda rwacu rukomera ku ihame shingiro rya "Ubwiza bushobora kuba ubuzima bwumuryango wawe, kandi izina rishobora kuba ubugingo bwarwo" kubiciro byiza kubushinwa Imikino ngororamubiri nubuvuzi Bikiza uruhu rwamagufwa na Tissue L-Glutamine Amino Acide, Urakoze gufata umwanya wawe mwiza wo kutugana kandi ugakomeza kugirana ubufatanye bwiza nawe.
Igiciro cyiza kubushinwa bwongeweho ibiryo, Imikino ngororamubiri, Isosiyete yacu isezeranya: ibiciro byiza, igihe gito cyo gukora na serivisi ishimishije nyuma yo kugurisha, turakwemera kandi gusura uruganda rwacu igihe cyose ubishakiye.Twifurije kubona ubucuruzi bushimishije kandi burebure hamwe !!!