Uruganda rwo mu Bushinwa Gutanga 2 X Byihuta Pfus PCR Master Yivanze na Pfu ADN Polymerase Dntps PCR Buffer Ihingura nuwitanga |Foregene

Gutanga Uruganda 2 X Byihuta Pfus PCR Master Yivanze na Pfu ADN Polymerase Dntps PCR Buffer

Ibisobanuro:

Koresha mu buryo butaziguye polysaccharide na polifenol amababi ya lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.

◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)

◮Ibikoresho bike

◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya

◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura

◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50

◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Turashimangira iterambere no kumenyekanisha ibicuruzwa bishya kumasoko buri mwaka kubitangwa ninganda 2 X Byihuta Pfus PCR Master mix hamwe na Pfu ADN Polymerase Dntps PCR Buffer, Mugihe tugenda dutera imbere, duhanze amaso ibicuruzwa byacu bigenda byiyongera kandi tunatezimbere ibigo byacu.
Turashimangira iterambere no kumenyekanisha ibicuruzwa bishya kumasoko buri mwaka kugirangoUbushinwa PCR Master mix na PCR Reagent, Dufite intego yo kuba ikigo kigezweho gifite intego yubucuruzi ya "Umurava nicyizere" kandi tugamije "Guha abakiriya serivisi zivuye ku mutima nibicuruzwa byiza".Turasaba tubikuye ku mutima inkunga yawe idahindutse kandi dushimira inama nziza nubuyobozi.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice I.	Buffer PS1
	Buffer P2
	6 Buffer Yipakurura ADN
Igice cya II	Buffer PS2
Igice cya II	2 × Ibibabi PCR Byoroshye^TMKuvanga

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Byoroheje-icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
■ 2 × Kuvanga, kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
Preparation Imyiteguro yihuse-yuzuye irashobora kurangira muminota 10, PCR reaction irashobora kurangira byibuze muminota 50
■ Ibisubizo byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Kumenyekanisha ibimera byahinduwe, genotyping, nibindi.

Icyitegererezo: amababi y'ibimera akungahaye kuri polysaccharide cyangwa polifenol

Ingano yicyitegererezo: Diameter 2-3mm (uburyo butaziguye), diameter 5-7mm (uburyo bwa lysis)

Urutonde rwo kumenya: ibicuruzwa bya PCR ≤1kb

Urujya n'uruza rw'akazi

ibibabi byibimera bitaziguye PCR wongeyeho-akazi

Amashanyarazi

ibibabi byibiti bitaziguye PCR wongeyeho Turashimangira iterambere no kumenyekanisha ibicuruzwa bishya kumasoko buri mwaka kubitangwa ninganda 2 X Byihuta Pfus PCR Master mix hamwe na Pfu ADN Polymerase Dntps PCR Buffer, Mugihe tugenda dutera imbere, duhanze amaso ibicuruzwa byacu bigenda byiyongera kandi tunatezimbere ibigo byacu.
Gutanga UrugandaUbushinwa PCR Master mix na PCR Reagent, Dufite intego yo kuba ikigo kigezweho gifite intego yubucuruzi ya "Umurava nicyizere" kandi tugamije "Guha abakiriya serivisi zivuye ku mutima nibicuruzwa byiza".Turasaba tubikuye ku mutima inkunga yawe idahindutse kandi dushimira inama nziza nubuyobozi.