Dufite kandi ubuhanga mu kunoza imicungire yibintu hamwe nuburyo bwa QC kugirango dushobore kugumana imbaraga ziteye imbere mubucuruzi buciriritse burushanwe kubucuruzi bwubusa kubushinwa CE / FDA Yemejwe na Magnetic Beads Method ADN / Rna Nucleic Acide Extraction Reagent Kits (Pre-packed Magnetic Beads), Kugira ngo tumenye ibyiza byoguhuza ibicuruzwa mu mahanga, mubucuruzi bwihuse hamwe nogutanga amakuru meza muburyo bwiza bwogutanga amakuru kubucuruzi bwisi yose.
Turimo kandi kunoza imicungire yibintu hamwe nuburyo bwa QC kugirango dushobore kugumana imipaka iteye imbere mubucuruzi buciriritse burushanwa cyane kuriAcide Nucleic Acide, Ubushinwa Nucleic Acide, Umusaruro wacu woherejwe mu bihugu n’uturere birenga 30 nkisoko yambere yintoki hamwe nigiciro gito.Twakiriye neza abakiriya baturutse mu gihugu ndetse no hanze kugirango baze kuganira natwe ubucuruzi.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo mer- mercaptoethanol (bidashoboka) .Turi kandi inzobere mugutezimbere imicungire yibintu hamwe nuburyo bwa QC kugirango dushobore kugumana umupaka uteye imbere mubucuruzi buciriritse burushanwe kubushakashatsi bwubusa kubushinwa CE / FDA Byemewe na Magnetic Beads Uburyo bukoreshwa neza isi yose mubijyanye no gutumanaho nabaguzi bo hanze, gutanga byihuse, ubuziranenge bwiza cyane nubufatanye burambye.
Icyitegererezo cyubusa kuriAcide Nucleic Acide, Ubushinwa Nucleic Acide, Umusaruro wacu woherejwe mu bihugu n’uturere birenga 30 nkisoko yambere yintoki hamwe nigiciro gito.Twakiriye neza abakiriya baturutse mu gihugu ndetse no hanze kugirango baze kuganira natwe ubucuruzi.