kubahiriza amasezerano ”, ahuza n'ibisabwa ku isoko, yinjira mu marushanwa yo ku isoko n'ubwiza bwayo kimwe nayo itanga ubufasha bwuzuye kandi buhebuje kubakiriya kugirango bareke babe abatsinze binini.Gukurikirana iyi sosiyete, byanze bikunze abakiriya bishimira Ubushinwa Ibicuruzwa bishya bigurishwa cyane virusi ya ADN & Rna Extraction Kit, Twaguye ubucuruzi bwacu mu Budage, Turukiya, Kanada, Amerika, Indoneziya, Ubuhinde, Nijeriya, Burezili ndetse no mu tundi turere tw’isi.Turimo gukora cyane kugirango tube umwe mubatanga isoko nziza.
kubahiriza amasezerano ”, ahuza n'ibisabwa ku isoko, yinjira mu marushanwa yo ku isoko n'ubwiza bwayo kimwe nayo itanga ubufasha bwuzuye kandi buhebuje kubakiriya kugirango bareke babe abatsinze binini.Gukurikirana isosiyete, byanze bikunze abakiriya bishimiraUbushinwa Rna & ADN Ikuramo Kit na Nucleic Acide, Dutegereje gushiraho umubano wunguka nawe dushingiye kubicuruzwa byacu byujuje ubuziranenge nibisubizo, ibiciro byumvikana na serivisi nziza.Turizera ko ibicuruzwa byacu bizakuzanira uburambe bushimishije kandi bitwara ibyiyumvo byubwiza.
Imfashanyigisho:Gutera Igiteranyo cya RNA Kwigunga Kit Wongeyeho Igitabo

kubahiriza amasezerano ”, ahuza n'ibisabwa ku isoko, yinjira mu marushanwa yo ku isoko n'ubwiza bwayo kimwe nayo itanga ubufasha bwuzuye kandi buhebuje kubakiriya kugirango bareke babe abatsinze binini.Gukurikirana iyi sosiyete, byanze bikunze abakiriya bishimira Ubushinwa Ibicuruzwa bishya bigurishwa cyane virusi ya ADN & Rna Extraction Kit, Twaguye ubucuruzi bwacu mu Budage, Turukiya, Kanada, Amerika, Indoneziya, Ubuhinde, Nijeriya, Burezili ndetse no mu tundi turere tw’isi.Turimo gukora cyane kugirango tube umwe mubatanga isoko nziza.
Ubushinwa Ibicuruzwa bishya Ubushinwa Rna & ADN Gukuramo Kit hamwe no Gukuramo Acide Nucleic, Dutegereje kuzashyiraho umubano mwiza hagati yawe nawe dushingiye kubicuruzwa byacu byiza kandi byiza, ibisubizo byiza na serivisi nziza.Turizera ko ibicuruzwa byacu bizakuzanira uburambe bushimishije kandi bitwara ibyiyumvo byubwiza.

Inkingi yacometse

Inkingi imaze gucomeka, umusaruro wa RNA uragabanuka cyangwa ntibishoboka koza RNA, kandi misa ya RNA yabonetse ni mike.

Isesengura rusange:

1. Ikiruhuko ntangarugero ntabwo cyuzuye.

Icyitegererezo cyo kumeneka ntabwo gitera rwose ADN-CLEANING COLUMN guhagarikwa, mugihe bigira ingaruka kumusaruro wa RNA nubwiza.Turasaba inama yo gusya byihuse muri azote ihagije mugihe wamennye ingero, Gerageza kumenagura urukuta rwicyitegererezo, membrane selile nizindi ngingo.Kubitegererezo byibihingwa bya polyol polysaccharide, turagusaba ko ukoresha Ibimera byose RNA ISOLATION KIT PLUS.

2. Iyo unywa icyitegererezo cyindengakamere cyatandukanijwe hamwe na ADN-Isukura Inkingi, imvura ishobora kuba igabanijwe ishobora guhumeka.

Ingirabuzimafatizo zacitsemo ibice zafashwe zizatera inkingi ya RNA-GUSA izahagarikwa mugihe ibikorwa bya RNA adsorption bikorwa (reba intambwe ya 6).Turagusaba ubwitonzi mugihe unywa iyi ndengakamere kugirango wirinde imyanda ya selile.

3. Icyitegererezo umubare wambere ni mwinshi.

Gukoresha urugero rwinshi bizavamo gutandukanya ibyitegererezo bituzuye cyangwa lysis selile ituzuye na Buffer PSL1, bikaviramo guhagarika inkingi yo kweza mugihe cyo kwezwa.Gutera Igiteranyo Cyuzuye RNA Kwigunga Buri cyitegererezo cyogukora ni 50 mg.Kubitegererezo byibihingwa bya polyol polysaccharide, turagusaba ko wagerageza Gutera Igiteranyo Cyuzuye RNA ISOLATION KIT PLUS.

4. Ubushyuhe bwa centrifuge buri hasi cyane.

Inzira yose ya RNA yo kwigunga no kwezwa ikorwa mubushyuhe bwicyumba (20-25°C), usibye ko ingero z'icyitegererezo zavunitse na azote yuzuye. Ubushyuhe bwa centrifuges zimwe na zimwe za kirogenike ziri munsi ya 20℃, zishobora gutera guhagarika ADN-Isukura Inkingi na / cyangwa RNA-Inkingi gusa.Niba ibi bibaye, shyira ubushyuhe bwa centrifuge kuri 20-25℃, namenya neza ko lysis ivanze na / cyangwa etanol yongeyeho supernatant yashushe kuri 37°C.

Nta RNA yakuweho cyangwa umusaruro wa RNA uri muke

Mubisanzwe hariho ibintu byinshi bigira ingaruka kumikorere yo kugarura, nka: icyitegererezo cya RNA, uburyo bwo gukora, ingano ya elution, nibindi.

Isesengura ry'impamvu zisanzwe nkuko bikurikira:

1.Ubwogero bwa ice cyangwa ubushyuhe buke (4 ° C) centrifugation yakozwe mugihe cyo kubaga.

Igitekerezo: Kora ku bushyuhe bwicyumba (15-25°C) mubikorwa byose, ntukore ubwogero bwa ice hamwe na centrifugation yubushyuhe buke.

2. RNA yateshejwe agaciro kubera kubika neza icyitegererezo cyangwa kubika igihe kirekire icyitegererezo.

Icyifuzo: Ingero zegeranijwe vuba zigomba gukonjeshwa vuba muri azote yuzuye, hanyuma zikabikwa kuri -80 ° C igihe kirekire, ukirinda gukonjesha no gukonjesha ingero;cyangwa uhite ushiramo ibyitegererezo muri RNA stabilisateur RNAlater igisubizo (icyitegererezo cyinyamaswa).

3.Ibice bidahagije byo gutandukanya na lysis biganisha ku guhagarika inkingi yo kwezwa.

Igitekerezo: Mugihe cyo gusya ingirangingo, nyamuneka urebe neza ko tissue iri hasi bihagije, kandi uyimure vuba muri Buffer PSL1 yateguwe mbere (wemeze ko igipimo gikwiye cya β-ME cyongeweho, reba intambwe ya 1 yuburyo).

4.Umuhanga wongeyeho nabi.

Igitekerezo: Menya neza ko RNase-idafite ddH2O yatonywe hagati yinkingi yo kweza.

5.Ubunini bukwiye bwa Ethanol yuzuye ntabwo yongewe kuri Buffer PSL2 cyangwa Buffer PRW2.

Igitekerezo: Nyamuneka kurikiza amabwiriza, ongeramo ingano yukuri ya Ethanol yuzuye kuri Buffer PSL2 na Buffer PRW2 hanyuma uvange neza mbere yuko ibikoresho bikoreshwa.

6. Ingano yicyitegererezo ntikwiye.

Igitekerezo: Koresha mg 50 za tissue kuri 500 μl ya Buffer PSL1.Gukoresha tissue nyinshi bizagabanya ingano ya RNA yakuweho kandi isuku ya RNA yavuyemo nayo izagabanuka.Turasaba cyane ko urugero rwambere rwicyitegererezo rutagomba kurenza mg 50 kubikorwa byo gukuramo RNA.

7.Ijwi ridakwiriye cyangwa gusohora kutuzuye.

Igitekerezo: Ingano yoroheje yinkingi yo kweza ni 50-200 μl;niba ingaruka zo gukuraho zidashimishije, birasabwa kongera igihe mubushyuhe bwicyumba nyuma yo kongeramo ubushyuhe bwa RNase-Free ddH2O, nka 5-10min.

8.Inkingi yo kweza ifite ibisigisigi bya Ethanol nyuma yo gukaraba hamwe na BufferPRW2.

Igitekerezo: Niba umuyoboro wubusa ushyizwemo min min 1 kandi haracyari etanol isigaye nyuma yo koza muri Buffer PRW2, urashobora kongera igihe cya centrifugation yubusa kugeza min 2, cyangwa ugashyira inkingi yo kweza mubushyuhe bwicyumba kuminota 5 kugirango ukureho Ethanol isigaye.

9.Ibikoresho byakoreshejwe nabi.

Icyifuzo: Kubihingwa by'icyitegererezo cya polifenolike polysaccharide, ukoresheje ibikoresho bisanzwe nka Plant Total RNA Isolation Kit ntibishobora kubona urugero rwiza rwa RNA.Turagusaba gukoresha Ikimera Cyuzuye RNA IsolationKit Plus, yagenewe byumwihariko kuburugero rwibiti bya polifenolike polysaccharide.Igikoresho cyakozwe muburyo bwo gukuramo RNA muri polifenol na polysaccharide yintangarugero.

OD260 / OD280 agaciro kari hasi

RNA ikuraho hamwe na ddH2O kandi ikoreshwa mugusoma spekitifotometero ibisubizo mubisubizo bya OD260 / OD280.Turasaba ko dukoresha mM 10 Tris-HCl, pH 7.5 (aho kuba RNase-Free ddH2O kugirango duhindure RNA) kugirango tubone indangagaciro za OD260 / OD280, reba "RNA Kwibanda no Kweza" kurupapuro rwa 19.

RNA yatunganijwe yangiritse

Ubwiza bwa RNA isukuye bujyanye nibintu nko kubungabunga icyitegererezo, kwanduza RNase, no gukoreshwa.

Isesengura ry'impamvu zisanzwe:

1.Icyitegererezo nticyabitswe mugihe cyo gukusanya.

Icyifuzo: Niba ingero za tissue zidakoreshejwe mugihe cyo gukusanya, nyamuneka uzibike muri azote yuzuye mumazi yubushyuhe buke ako kanya cyangwa uyohereze kuri -80 ° C kugirango ubike igihe kirekire nyuma yo gukonjeshwa vuba muri azote yuzuye, cyangwa uhite wibiza ibyitegererezo muri RNA stabilizer RNAlater igisubizo (icyitegererezo cyinyamaswa).Kubikuramo RNA, gerageza gukoresha ingero za tissue nshya zegeranijwe.

2.Gusubiramo kenshi no gukonjesha ingero za tissue.

Icyifuzo: Iyo ubitse ingero za tissue, nibyiza kubicamo uduce duto kugirango tubungabunge, hanyuma ugakuramo igice cyabyo mugihe ubikoresheje kugirango wirinde kwangirika kwa RNA guterwa no gukonjesha no gukonjesha ingero.

3.RNase yatangijwe mucyumba cyo gukoreramo cyangwa ntabwo yambarwa na gants imwe, masike, nibindi.

Icyifuzo: Ubushakashatsi bwo gukuramo RNA bukorwa neza mubikorwa bitandukanye bya RNA, kandi ameza ya laboratoire agomba gusukurwa mbere yubushakashatsi, kandi uturindantoki twajugunywe hamwe na masike bigomba kwambarwa mugihe cyibigeragezo kugirango twirinde kwangirika kwa RNA guterwa no kwinjiza RNase ku rugero runini.

4.Ragent yanduye na RNase mugihe cyo kuyikoresha.

Icyifuzo: Simbuza urukurikirane rushya rwibimera byose RNA ikuramo ibikoresho kubushakashatsi bujyanye.

5.Icyuma cya centrifuge hamwe na pipette inama zikoreshwa muri RNA manipulation zanduye na RNase.

Igitekerezo: Menya neza ko imiyoboro ya centrifuge, inama za pipeti, imiyoboro, nibindi bikoreshwa mugukuramo RNA byose ni RNase-Ubuntu.

Imfashanyigisho:

Gutera Igiteranyo cya RNA Kwigunga Kit Wongeyeho Igitabo