Hamwe niyi ntego, twahindutse rwose kuba umwe mubakora udushya twikoranabuhanga, dukoresha amafaranga menshi, kandi duhatanira ibiciro kuri Top GradeUbushinwa Gukuramo Acide Nucleiccyangwa Isuku Kit spin inkingi yuburyo, Ubu tumaze imyaka irenga 10 dukora.Twiyeguriye ibicuruzwa byiza kandi bisubizo hamwe nubufasha bwabaguzi.Turagutumiye guhagarika byanze bikunze ubucuruzi bwacu kugirango tuzenguruke kugiti cyawe no kuyobora ibigo byateye imbere.
Hamwe niyi ntego, twahindutse rwose kuba umwe mubakora udushya mu ikoranabuhanga, dukoresha amafaranga menshi, kandi duhatanira ibiciro kuriUbushinwa Gukuramo Acide Nucleic, Inkingi Inkingi Uburyo, Twashizeho umubano ukomeye kandi muremure mubufatanye hamwe nisosiyete nini muri ubu bucuruzi mumahanga.Serivise ako kanya ninzobere nyuma yo kugurisha itangwa nitsinda ryacu ryabajyanama ryishimiye abaguzi bacu.Byimbitse Amakuru nibipimo biva mubicuruzwa birashoboka koherezwa kubwawe kugirango ubyemere neza.Ingero z'ubuntu zirashobora gutangwa kandi isosiyete ikagenzura isosiyete yacu.n Porutugali kugirango imishyikirano ihora ikaze.Twizere kubona anketi wandike kandi wubake ubufatanye bwigihe kirekire.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yujuje ubuziranenge RNA yose mubice bitandukanye byibimera bifite polysaccharide nkeya hamwe na polifenol.Kubitegererezo byibihingwa bifite polysaccharide nyinshi cyangwa polifenole, birasabwa gukoresha Plant Total RNA Isolation Plus Kit kugirango ubone ibisubizo byiza byo gukuramo RNA.Igikoresho gitanga ADN-Isukura inkingi ishobora gukuraho ADN genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nkeya hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) ahantu humye, na 2-8 ℃ mugihe kirekire (amezi 24).

Buffer PSL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka). Hamwe niyi nteruro mubitekerezo, twabonye rwose ko turi umwe mubakora ubuhanga bugezweho bwa tekinoloji, buhendutse, kandi burushanwe kubiciro bya Top GradeUbushinwa Gukuramo Acide Nucleiccyangwa Igikoresho cyo Kweza (Magnetic Bead Method), Ubu tumaze imyaka irenga 10 dukora.Twiyeguriye ibicuruzwa byiza kandi bisubizo hamwe nubufasha bwabaguzi.Turagutumiye guhagarika byanze bikunze ubucuruzi bwacu kugirango tuzenguruke kugiti cyawe no kuyobora ibigo byateye imbere.
Isonga rya mbere mu Bushinwa Gukuramo Acide Nucleic Acide, Uburyo bwa Magnetic Bead Method, Twubatse umubano ukomeye kandi muremure mubufatanye hamwe namasosiyete menshi muri ubu bucuruzi mumahanga.Serivise ako kanya ninzobere nyuma yo kugurisha itangwa nitsinda ryacu ryabajyanama ryishimiye abaguzi bacu.Byimbitse Amakuru nibipimo biva mubicuruzwa birashoboka koherezwa kubwawe kugirango ubyemere neza.Ingero z'ubuntu zirashobora gutangwa kandi isosiyete ikagenzura isosiyete yacu.n Porutugali kugirango imishyikirano ihora ikaze.Twizere kubona anketi wandike kandi wubake ubufatanye bwigihe kirekire.