Yubahiriza amahame "Inyangamugayo, abanyamwete, abanyamwete, bashya" kugirango bateze imbere ibicuruzwa bishya buri gihe.Ireba abakiriya, intsinzi nkitsinzi yayo.Reka dutezimbere ejo hazaza heza mu ntoki kubicuruzwa byinshi bya spin Metro Nucleic Acide Gukuramo Reagent Bottle Kits, Turashobora kukugezaho kimwe mubiciro byo kugurisha birushanwe kandi bifite ireme, kuko twarushijeho kuba twujuje ibyangombwa!Ntugomba rero gutindiganya kuduhamagara.
Yubahiriza amahame "Inyangamugayo, abanyamwete, abanyamwete, bashya" kugirango bateze imbere ibicuruzwa bishya buri gihe.Ireba abakiriya, intsinzi nkitsinzi yayo.Reka dutezimbere ejo hazaza heza mu ntokiUbushinwa Gukuramo Acide Nucleic Kit (spin inkingi), Kwipimisha Acide Nucleic, Gushiraho igihe kirekire no gutsindira inyungu mubucuruzi nabakiriya bacu bose, dusangire intsinzi kandi tunezerwe no gukwirakwiza ibicuruzwa byacu kwisi hamwe.Twizere kandi uzunguka byinshi.Nyamuneka nyamuneka kutwandikira kubindi bisobanuro, turabizeza ko tuzabitaho igihe cyose.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yujuje ubuziranenge RNA yose mubice bitandukanye byibimera bifite polysaccharide nkeya hamwe na polifenol.Kubitegererezo byibihingwa bifite polysaccharide nyinshi cyangwa polifenole, birasabwa gukoresha Plant Total RNA Isolation Plus Kit kugirango ubone ibisubizo byiza byo gukuramo RNA.Igikoresho gitanga ADN-Isukura inkingi ishobora gukuraho ADN genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nkeya hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) ahantu humye, na 2-8 ℃ mugihe kirekire (amezi 24).

Buffer PSL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka) .Bikurikiza amahame "Inyangamugayo, abanyamwete, abanyamwete, bahanga udushya" kugirango batezimbere ibicuruzwa bishya buri gihe.Ireba abakiriya, intsinzi nkitsinzi yayo.Reka dutezimbere ejo hazaza heza kubiguzi byinshi Ubushinwa Magnetic Beads Method Nucleic Acide Detection Testing Extraction Diagnostic Reagent Bottle Kits, Turashobora kukugezaho kimwe mubiciro byo kugurisha birushanwe kandi bifite ireme, kuko twarushijeho kuba twujuje ibisabwa!Ntugomba rero gutindiganya kuduhamagara.
Igiciro Cyinshi Ubushinwa Gukuramo Acide Nucleic Acide (amasaro ya magneti),Kwipimisha Acide Nucleic, Gushiraho igihe kirekire no gutsindira inyungu mubucuruzi nabakiriya bacu bose, dusangire intsinzi kandi tunezerwe no gukwirakwiza ibicuruzwa byacu kwisi hamwe.Twizere kandi uzunguka byinshi.Nyamuneka nyamuneka kutwandikira kubindi bisobanuro, turabizeza ko tuzabitaho igihe cyose.