Hariho ubwoko bubiri bwingenzi bwibyago muri laboratoire ya PCR: ibyago bya biosafety hamwe na aside nucleique ishobora kwanduza.Iyambere yangiza abantu nibidukikije, naho iyanyuma igira ingaruka kubisubizo bya PCR.Iyi ngingo ivuga kubyerekeye amanota ya laboratoire ya PCR hamwe nurwego rujyanye ningaruka zazanwe.

01 Igabana rya laboratoire ya PCR

1. Laboratoire yo gupima ibinyabuzima

Ukurikije ibisabwa mu ngingo ya 1.1 yubuziranenge bwibanze bwo gushyiraho Laboratwari zipima Clinical Gene Amplification, Laboratoire ya PCR muri rusange igizwe nibice bine: ahantu ho kubika no gutegura, ahantu hateganijwe gutegurwa, ahakorerwa amplification, hamwe n’isesengura ry’ibicuruzwa.Niba uburyo nyabwo bwa fluorescent PCR bwakoreshejwe, agace ka amplification hamwe nisesengura rishobora guhurizwa mukarere kamwe;niba isesengura ryuzuye rya PCR ryakoreshejwe, ahantu hateganijwe gutegurwa, agace ka amplification hamwe nisesengura rishobora guhurizwa mukarere kamwe.

“Igitabo gikubiyemo imirimo yo gupima aside irike ya Coronavirus Nucleic Acide mu bigo by’ubuvuzi (Ikigeragezo cya kabiri)Ibi bice bitatu bigomba kwigenga rwose mumwanya wumubiri, kandi ntihashobora kubaho itumanaho ritaziguye numwuka.

Icyumba cyo gutegura

Nubwo ibyitegererezo bishobora gutegurwa gusa ahantu hateganijwe gutegurwa, icyumba cyihariye cyo gutegura icyitegererezo kiracyakenewe mugihe cyo guhangana nicyitegererezo hamwe numubare munini wicyitegererezo.Icyumba cyo gutegura icyitegererezo gifite ibyago byinshi byumutekano wibinyabuzima no kwanduza aside nucleique.

3. Icyumba cyo gutunganya imyanda

Gutunganya imyanda idakwiye kandi bizana ingaruka zikomeye zo kwanduza biosafeti na aside nucleique muri laboratoire.Kubwibyo, icyumba cyo gutunganya imyanda kigomba gukurikiranwa buri gihe.

02 Ingingo zo gukurikirana ingaruka muri laboratoire ya PCR

Laboratoire zitandukanye zigabanyijemo icyumba cyo gutegura icyitegererezo, ububiko bwa reagent hamwe n’ahantu ho gutegurira, ahantu ho gutegurira ingero, amplification hamwe n’isesengura ry’ibicuruzwa, n’icyumba cyo gutunganya imyanda.

Ukurikije ubwoko bwikitegererezo, igabanijwemo ubuso, ibikoresho, icyitegererezo, gukurikirana ibidukikije na pipette.

Urwego rwibyago ruva hasi kugeza hejuru kuva inyenyeri imwe ★ kugeza ku nyenyeri eshatu ★★★.

1. Icyumba cyo gutegura icyitegererezo:

Ikoreshwa mukwiyandikisha, gutegura no kudakora ingero, kandi ibyago byumutekano wibinyabuzima nibyo byinshi.Kuberako ibyitegererezo bidakuwe kandi byongerewe imbaraga, usibye imiyoboro ikunze guhura nintangarugero, ibyago byo kwanduza aside nucleique mubindi bice ni bike.

1-4 Gutoranya ahakurikiranwa

5-8 Gutoranya ahakurikiranwa

9-12 yo gukurikirana ingingo

1. Ahantu ho kubika no gutegura:

Ikoreshwa mugutegura ububiko bwa reagent, gutanga reagent no gutegura imvange ya amplification reaction, hamwe no kubika no gutegura ibikoreshwa nkibikoresho bya centrifuge hamwe ninama za pipette.Ntaho uhurira nintangarugero kandi nta aside nucleique nziza muri kariya gace, bityo ibyago bya biosafety hamwe na aside nucleic ibyago byo kwanduza ni bike.

13-16 Gutoranya ahakurikiranwa

17-22 Icyitegererezo mugukurikirana
3. Icyitegererezo cyo gutegura areang ingingo

Ikoreshwa mugukingura ingunguru yo kwimura, kudakora urugero (mugihe bibaye ngombwa), gukuramo aside nucleic no kuyongerera mumiyoboro ya amplification reaction, nibindi. Aka gace gashobora kuba karimo gutunganya no gufungura ingero, ibyago byumutekano wibinyabuzima ni byinshi, kandi gukuramo aside nucleique birakorwa, kandi ibyago byo kwanduza aside nucleique ni bito kugeza hejuru.

29 Gutoranya ahakurikiranwa

4. Amplification hamwe nisesengura ryibicuruzwa:

Ikoreshwa muri nucleic aside amplification.Aka karere ntikubiyemo gutunganya icyitegererezo, kandi umutekano wibinyabuzima ni muke.Kwiyongera kwa acide nucleic ahanini biri muri kano karere, kandi ibyago byo kwanduza aside nucleique nibyo byinshi.

38 Gutoranya ahakurikiranwa
5. Icyumba cyo gutunganya imyanda:

Byakoreshejwe mugutunganya umuvuduko mwinshi wintangarugero.Ingaruka z’umutekano w’ibinyabuzima zigira uruhare mu gutunganya ingero muri kariya gace ni nyinshi.Ibicuruzwa byongera aside nucleic birasabwa gufatwa nkimyanda yubuvuzi.Umuvuduko mwinshi ntusabwa, kandi ibyago byo kwanduza aside nucleic ni bike.

43-44 Gutoranya ahakurikiranwa

03 Shyira mu bikorwa

Iki gihe twashyizeho urutonde 44 rwo gukurikirana.Bigereranijwe ko abantu benshi bagomba kubaza, bakeneye gukora ingingo nyinshi?Yego, kora byose!Ndagusaba ko wabanje gukora isuzuma ryibyago bya laboratoire yawe bwite, ibyo bikaba byakorwa ukurikije ingaruka ziva hejuru kugeza hasi, urashobora kandi gukurikirana ubwoko bumwe bwikitegererezo hamwe, cyangwa ugashobora gutegura gahunda yicyitegererezo yo gukurikirana buri gihe.Muri make, buri laboratoire irashobora gukora gahunda yayo yo kuyishyira mu bikorwa ishingiye ku bihe byayo.Ingaruka nini yo gupima laboratoire ni ukwirengagiza ingaruka.