Iriburiro:

Molekile ya RNA irashobora gukorana cyane nizindi molekile yibinyabuzima, nka poroteyine, ADN na RNA, bityo ikabaho mu ngirabuzimafatizo no mu binyabuzima mu buryo bwa macromolecular.Muri byo, ibigo bya RNA-protein ni ukubaho n'imikorere ya RNA Imwe muburyo bwingenzi bwa.RNA gukuramo ni uburyo bwa kera bwo gusuzuma no kumenya molekile ya RNA, cyane cyane poroteyine zikorana na molekile ya lncRNA, kandi izwi na benshi mu bashakashatsi ndetse n’ibinyamakuru mpuzamahanga byemewe.Uyu munsi FORREGENE izagusangiza nawe ihame ryigeragezwa hamwe na tekiniki ya RNA gukuramo, wenda ushobora kuyikoresha

1. Ihame ryubushakashatsi bwa RNA rikuraho ikoranabuhanga:

Muri vitro transcription hamwe na synthesis ya biotin yanditseho intego lncRNA cyangwa lncRNA ibice (harimo ibice bya RNA bikoreshwa nkigenzura ryiza kandi ribi), hamwe na incubate hamwe nibisohoka;koresha amashanyarazi ya avidin-anchore kugirango ukusanyirize hamwe RNA-protein cyangwa RNA-RNA, Nyuma yo gukaraba neza, uruganda rurakurwa.Niba umenye intungamubiri za poroteyine zikorana na lncRNA, kora poroteyine electrophoreis ku bicuruzwa bikurura RNA, menya poroteyine yagenewe ukoresheje ifeza, hanyuma ukate gele ya sprometrike;nanone gukora mu buryo butaziguye igenzura ryiburengerazuba ku bicuruzwa bikurura;niba byamenyekanye Kuri molekile ya RNA ikorana na lncRNA, ibicuruzwa bikurura RNA bikururwa na RNA kandi hakorwa qRT-PCR yihariye.

2. RNA ikuraho inzira yubushakashatsi bwikoranabuhanga:

3. Serivise ya tekinike ya RNA ikuramo FOREGENE:

Umuco w'akagari

Gutegura ingirabuzimafatizo zose / ibisasu bya kirimbuzi / plasma

RNA ikuramo igerageza

SDS-PAGE electrophoreis, irangi rya feza

Mass spectrometry hamwe nubushakashatsi bwa WB

Raporo yubushakashatsi

4. Urubanza

Igishushanyo gikurikira cyerekana ibisubizo bya FJ Bio-Bio ya RNA ikuramo hasi na SDS-PAGE ya electrophoreis, aho umwambi utukura uranga itandukaniro:

Itsinda ry’ubushakashatsi bwa Porofeseri Song Xu wo muri kaminuza ya Sichuan ryakusanyije imyaka 15 yo kwirundanya igihe kirekire mu rwego rw’ubushakashatsi bwa lncRNA.Itsinda ry’ubushakashatsi bwa Foregene Bio na Porofeseri Song Xu bafatanije gutangiza RNA gukuramo ikoranabuhanga rya lncRNA, bizaguha ibisubizo bishimishije kubushakashatsi bwawe.