Mu myaka icumi ishize, tekinoroji yo guhindura gene ishingiye kuri CRISPR yateye imbere byihuse, kandi ikoreshwa neza mu kuvura indwara zikomoka kuri kanseri na kanseri mu bigeragezo by’amavuriro.Muri icyo gihe, abahanga ku isi bahora bashakisha ibikoresho bishya bifite ubushobozi bwo guhindura gene kugira ngo bakemure ibibazo by’ibikoresho bisanzwe byo guhindura no gufata ibyemezo.

Muri Nzeri 2021, itsinda rya Zhang Feng ryasohoye urupapuro mu kinyamakuru Science [1], basanga abantu benshi batwara abantu banditse RNA bayobora imisemburo ya nucleic aside maze bayita sisitemu ya Omega (harimo ISCB, ISRB, TNP8).Ubushakashatsi bwagaragaje kandi ko sisitemu ya Omega ikoresha igice cya RNA mu kuyobora ibice bibiri bya ADN, aribyo ωRNA.Icy'ingenzi cyane, iyi misemburo ya acide nucleic ni nto cyane, hafi 30% ya CAS9, bivuze ko ishobora kuba yagejejwe kuri selile.

Ku ya 12 Ukwakira 2022, itsinda rya Zhang Feng ryasohoye mu kinyamakuru Kamere cyiswe: Imiterere ya Omega Nickase ISRB mu kigo hamwe na ωrna na ADN ya ADN [2].

Ubushakashatsi bwongeye gusesengura imiterere ya microscope ya elegitoronike ya ISRB-ωRNA hamwe na ADN igizwe na sisitemu ya Omega.

ISCB ni sekuruza wa CAS9, kandi ISRB nikintu kimwe cyo kubura aside HNH nucleic aside ya ISCB, ubunini rero ni buto, hafi acide amine 350 gusa.ADN itanga kandi umusingi wo kurushaho gutera imbere no guhindura inganda.

IsrB iyobowe na RNA ni umwe mu bagize umuryango wa OMEGA ushyizweho na IS200 / IS605 birenze urugero bya transposons.Uhereye ku isesengura rya phylogeneque hamwe na domaine zidasanzwe, IsrB birashoboka ko ibanziriza IscB, sekuruza wa Cas9.

Muri Gicurasi 2022, Laboratoire ya Lovely Dragon University ya Cornell yasohoye urupapuro mu kinyamakuru Science [3], isesengura imiterere ya IscB-ωRNA n'uburyo bwo guca ADN.

Ugereranije na IscB na Cas9, IsrB ibuze indangarugero ya HNH nuclease, REC lobe, hamwe na domaine nyinshi za PAM zikurikirana, bityo IsrB ni nto cyane ugereranije na Cas9 (acide zigera kuri 350 gusa).Nyamara, ingano ntoya ya IsrB iringanizwa nubuyobozi bunini ugereranije RNA (omega RNA yayo ni 300 nt z'uburebure).

Itsinda rya Zhang Feng ryasesenguye imiterere ya microscope ya cryo-electron ya IsrB (DtIsrB) ikomoka kuri bacterium ya damp-ubushyuhe bwa anaerobic Desulfovirgula thermocuniculi hamwe na complexe ya ωRNA hamwe na ADN igamije.Isesengura ryuburyo ryerekanye ko imiterere rusange ya poroteyine IsrB yasangiye imiterere yumugongo na proteine ​​ya Cas9.

Ariko itandukaniro nuko Cas9 ikoresha REC lobe kugirango yorohereze kumenyekanisha intego, mugihe IsrB yishingikirije kuri ωRNA yayo, igice cyacyo kigizwe nuburyo bugoye butatu bukora nka REC.

Kugira ngo dusobanukirwe neza impinduka zuburyo bwa IsrB na Cas9 mugihe cyubwihindurize kuva RuvC, itsinda rya Zhang Feng ryagereranije imiterere ya ADN ihuza intego ya RuvC (TtRuvC), IsrB, CjCas9 na SpCas9 yo muri Thermus thermophilus.

Isesengura ryimiterere ya IsrB na ωRNA irasobanura uburyo IsrB-ωRNA ifatanya kumenya kandi igahuza ADN igamije, kandi ikanatanga urufatiro rwo kurushaho guteza imbere nubuhanga bwiyi nuclease.Kugereranya nubundi buryo buyobowe na RNA bugaragaza imikoranire yimikorere hagati ya poroteyine na RNAs, bigatera imbere gusobanukirwa ibinyabuzima nihindagurika rya sisitemu zitandukanye.

Ihuza:

1.https://www.ubuhanga.org/doi/10.1126/ubuhanga.abj6856

2.https: //www.ubuhanga.org/doi/10.1126/ubuhanga.abq7220

3.https: //www.umuco.com/articles/s41586-022-05324-6