Waba uri mushya mubuzima bwibinyabuzima, cyangwa umutware mukuru ufite ubuhanga nimbaraga zikomeye, wigeze wirirwa ukuramo selile yuzuye ya RNA yuzuye?Nabantu bangahe batakaza ingero zagaciro kubera imikorere igoye kandi itoroshye yuburyo gakondo bwa Trizol?

Noneho, ubutumwa bwiza buraza!Sezera byimazeyo uburyo butwara igihe kandi budakora neza, kandi ukuramo byoroshye RNA yuzuye murwego rwo hejuru muminota 16.

Akagari Igiteranyo cya RNA Igikoresho cyo kwigunga

Sisitemu ntigira RNase, ntikeneye gukuramo chloroform, nta proteine ​​nibindi byanduye, urashobora rero kwizeza.

Waba uri mushya mushya cyangwa umuyobozi mukuru, uzagira amahame yawe yo hejuru kugirango ubuziranenge bwo gukuramo RNA.

None ni ubuhe bwoko bw'igikoresho cyiza?

Muri make, bisobanura “byinshi kandi byiza.”

Ntakibazo, uhaze umururumba wawe muto.

Iki gikoresho gishobora gukuramo RNA yose muri selile zifite muri plaque 96, 24, 12, na 6-nziza hamwe nibisohoka byinshi.Hariho ubwoko bwinshi bwa RNA yagaruwe.Usibye ibice bisanzwe 28 na 18s, 5s nibice bito bya miRNA birashobora kandi gukoreshwa.Byegeranijwe.

Inkingi idasanzwe yoza ADN igufasha gukuraho ADN genomic utongeyeho DNase, kandi ingaruka ni nziza, wujuje ibyifuzo byawe byubushakashatsi.

Urebye hano, abantu bose barimuwe?Ntugire ikibazo, ibicuruzwa byiza, vuga nukuri!!!

一 、 Biroroshye!(16min ntabwo ari kuvuga gusa)

二 quality Ubwiza bwo hejuru!(Ingaruka iragaragara)

Foregene Cell Yuzuye RNAIsolation Kits Kugereranya ibisubizo byuburyo bwa adsorption nuburyo bwa Trizol

三 、 Birahamye!(Numara kuyikoresha izavuga ko ari byiza)

Foregene QPCR ishusho ya gene A nyuma yo kwandukura

Inama:

Iyo ingano ya selile iba mike cyane, umusaruro wa RNA wose uzagabanuka, kandi spekitifotometero ntishobora kumenya neza agaciro ka OD.Birasabwa gukora ibizamini byiza binyuze mubizamini bya qPCR.Iki gicuruzwa gikorana neza na sosiyete yacu ya RT EasyTM II (Synthesis ya mbere ya cDNA ya Real Time PCR) (RT-01022)!

Hitamo Foregene Yumuco Yumudugudu Igiteranyo cya RNA Ikuramo kugirango igerageze intambwe imwe yihuse.Utegereje iki, kora nonaha!