Gushyushya-gutangira Taq enzyme ikoreshwa cyane.Ugereranije na polymerase isanzwe ya ADN, ishyushye-itangira Taq enzyme irashobora kwirinda neza uburyo bumwe na bumwe budasanzwe bwo kwongera no gushiraho primer dimers, kandi birashobora kuzamura neza igipimo cyo gutsinda kwa gene amplification.Cyane cyane mubijyanye no gupima genetike, hot-itangira Taq enzyme yamenyekanye nkigipimo giteganijwe mu nganda, kandi polymerase isanzwe ya ADN ntigomba gukoreshwa.Nkuko bigaragara mubyavuzwe haruguru, bishyushye-gutangira Taq enzymes zikoreshwa cyane.Kugeza ubu, hari ibirango byinshi bishyushye-bitangira imisemburo ya Taq ku isoko ryimbere mu gihugu, ariko ntihariho imisemburo myinshi ya Taq ishyushye ifite ubuziranenge.Guhura nibicuruzwa byinshi bishyushye-bitangiza Taq enzyme, twahitamo dute?

1. Hitamo hot-tangira enzyme ya Taq hamwe na amplification yo hejuru

Gukora neza kwa PCR bifitanye isano rya hafi nimikorere ya Taq enzyme.Nyuma ya sisitemu nziza ya Taq enzyme yogukora neza, imikorere ya amplification iri hejuru ya 95%, kandi amplification intera yumubare wambere wicyitegererezo ni nini.Amplifisione ishimishije irashobora kuboneka mugihe intego ya gen igenewe ari mike, kandi ntabwo byoroshye kuroga mugihe urugero rwicyitegererezo ari rwinshi, kandi igihe cyo kwongerera igihe kirekire ni kirekire.Kuri enzyme ya Taq ifite imikorere idahwitse, niyo sisitemu yogukora yaba yarahinduwe neza inshuro nyinshi, imikorere ya amplification iracyari munsi ya 90%, imiterere ya "S" yumurongo wa amplification ntago igaragara, ahahanamye ni hato, kandi umurongo ureshya.Iyo ingano yicyitegererezo ari mike, ntishobora kongererwa imbaraga, kandi mugihe ingano yicyitegererezo ari myinshi, ingaruka zo kongera imbaraga ntabwo ari nziza.Kubwibyo, gutoranya polymerase ya ADN ifite ubushobozi bwo kongera imbaraga ni ngombwa kugirango PCR na qPCR bigerweho.

2. Hitamo ashyushye-gutangira Taq enzyme ifite imbaraga zikomeye za enzyme

Imbaraga za enzymatique ya Taq enzyme ifitanye isano no gukora neza.Mubisanzwe, imbaraga za enzymatique zingufu zishyushye-zitangira Taq enzyme, nigihe kinini cyo gukura kwinshi kwa PCR kwongererwa imbaraga, uko bisanzwe 'S-shusho' umurongo, niko ibimenyetso bya fluorescence bifite agaciro, kandi bikwiranye no kumenya PCR nyinshi.Ikirangantego cya ADN polymerase ifite imbaraga zidasanzwe zidasanzwe zishobora gushyigikira gusa reaction ya 2-plex.Iyo ukora reaction ya 3-plex, amplification curve iba mike, ibimenyetso bya fluorescence agaciro ni muke, kandi ntanumwanya uhari wa amplification uteganijwe, ibisubizo rero biragoye kubicira urubanza.

3. Hitamo ashyushye-utangire Taq enzyme hamwe na sensibilité yo hejuru

Muri rusange, ADN polymerase ifite ubushobozi bwo kongera imbaraga no kumva neza, ariko hariho n'ibidahuye.Niba intego ya gene ubwinshi bwikitegererezo igomba kongerwaho ari mike, birasabwa kugerageza amplification sensibilité ya Taq enzyme.Uburyo busanzwe bwo gutahura ni ugukora inshuro 10 cyangwa inshuro 5 zo kugabanya icyiciro cya gene plasmid igenewe, gukora PCR gutahura mugihe cyo hasi, hanyuma ugahitamo umusemburo wa Taq enzyme ushyushye hamwe no kumva neza.

Birashobora kugaragara uhereye hejuru ko abashakashatsi bakeneye guhitamo bakurikije ibyifuzo byabo byubushakashatsi hamwe nuburyo inkunga yatanzwe.Nibyiza gukora igeragezwa rya dilution ya amplification igerageza kugirango umenye imikorere ya amplification hamwe na sensibilité ya hot-itangira Taq enzyme.

Urugero rwa Foregene's Taq ADN Polymerase:

Foreasy HS Taq ADN Polymerase

Ibisobanuro

Foreasy HS Taq ADN Polymerase ni polymerase ya ADN igaragara muri bagiteri ya Escherichia coli yubukorikori hakoreshejwe tekinoroji ya gene.Enzyme ihujwe na buffffer idasanzwe, ituma ibicuruzwa birwanya cyane kandi bigahuza, kandi birashobora gukoresha mu buryo butaziguye urugero rwa lysate (sisitemu ya Foregene Lysis) nk'icyitegererezo cyo gutahura ibisubizo.

Gusaba

PCR yujuje ubuziranenge hamwe na PCR igereranya yerekana inyandikorugero zeze hamwe na templates zitanduye.

Kugenzura ubuziranenge

1.Nta gikorwa cya nuclease exogenous cyagaragaye

2.PCR uburyo bwo kumenya ADN isigaye idafite ADN

3.Bishobora kwongerera imbaraga ingirabuzimafatizo imwe muri Genome yumuntu

4.Bika ubushyuhe bwicyumba icyumweru, ibikorwa bidahinduka

Ibisobanuro byibicuruzwa: https://www.foreivd.com/impamvu-hs-taq-dna-polymerase-product/