Kubaka SOP Systerm

Gushiraho PCR igerageza SOP kugirango igenzure imyitwarire yabakozi bakora ubushakashatsi.

Abashakashatsi bubahiriza byimazeyo imikorere, kandi bagabanya umwanda wa PCR ushobora guterwa nimpamvu zabantu cyangwa bakirinda ko habaho umwanda muri iki gikorwa.Byongeye kandi, uwagerageje agomba kuba afite ubumenyi nubuhanga bujyanye n’umwuga, harimo ubuhanga mu bikoresho bifitanye isano no gukora, gusobanura inzira zose z’akazi, kumenya uburyo bwo kuvura bwanduye hamwe n’uburyo bwo kugenzura ubuziranenge bwa laboratoire, kandi agashobora gusobanura neza ibyavuye mu kizamini.

Kubaka Laboratoire isanzwe ya PCR

Laboratoire ya PCR igabanijwemo ibice bine muburyo bwihariye, aribwo agace gategura reagent, agace gatunganyirizwamo icyitegererezo, agace kongerewe imbaraga, hamwe n’isesengura ry’ibicuruzwa.Ibice bibiri byambere ni pre-amplification ahantu, naho ibice bibiri byanyuma ni nyuma yinyongera.Agace kibanziriza amplification na zone ya nyuma ya amplification bigomba gutandukana rwose.Ibikoresho byubushakashatsi, reagent, impapuro zandika, amakaramu, ibikoresho byogusukura, nibindi birashobora gutemba gusa kuva ahantu habanziriza amplification kugera kumwanya wanyuma wa amplification, ni ukuvuga kuva ahantu hateganijwe reagent, ahantu hatunganyirizwa icyitegererezo, ahakorerwa amplification, hamwe no gusesengura ibicuruzwa, kandi ntibigomba gutemba muburyo butandukanye.Umwuka wo muri laboratoire ugomba kandi gutemba uva ahabanza kwongerwaho imbaraga ukajya nyuma yinyongera, ntabwo ari muburyo bwinyuma.

Mugabanye intambwe zigeragezwa

Niba laboratoire ikora gusa PCR no kumenya, irasabwa gukoresha PCR igereranya PCR aho gukoresha PCR isanzwe.

Ibisubizo bya Fluorescence byerekana PCR birashobora gukusanywa no gusesengurwa nibimenyetso bya fluorescent, kubwibyo rero nta mpamvu yo gukingura umupfundikizo wa electrophoreis nyuma yo kubyitwaramo, birinda kwanduza ibicuruzwa bya PCR biterwa no kumeneka kw'ibicuruzwa biva mu kirere bikora aerosole.Niba wongeyeho umubare wugurura cap mugihe cyo gupakira gel electrophorei, kwanduza aerosol birashoboka.Birasabwa guteza imbere ikoreshwa rya PCR igereranije no gusimbuza buhoro buhoro PCR.

Kwemeza gahunda ya UNG yo kurwanya umwanda

Sisitemu yo kwanduza ibicuruzwa UNG irwanya PCR ikoreshwa mubitekerezo bya PCR.

Sisitemu ikoresha dUTP aho gukoresha dTTP.Nyuma ya PCR reaction, ibicuruzwa byose bya PCR (ibice bya ADN) byashizwemo na dUTP;mugice gikurikiraho cya PCR reaction, enzyme ya UNG yongewe kuri sisitemu yashyizwe kuri 37 ° C muminota 5 mbere ya PCR, ishobora kuba Degrade yihariye ibice byose bya ADN birimo dUTP, hanyuma bigakora PCR.Ibi birashobora gukuraho burundu kwanduza aerosol guterwa nibicuruzwa bya PCR.Ingaruka zerekanwa ku gishushanyo gikurikira:

Icyitonderwa: Kuburyo bwa PCR butaziguye, urashobora guhitamo ibicuruzwa bikurikirana bya sisitemu yo kurwanya ibicuruzwa byangiza PCR bya FJ Biotech

Tanga igitekerezo

Kuri laboratoire ikora ibizamini binini bya genotyping, birasabwa cyane gukoresha sisitemu yo kwanduza UNG anti-PCR kugirango yipime reagent hiyubakwa laboratoire zifatika.

Kwibutsa: Gukoresha iyi sisitemu ntibishobora gukuraho ibicuruzwa byanduye PCR bimaze guterwa.Kubwibyo, sisitemu ya UNG igomba gukoreshwa mugitangira ikizamini kijyanye, kandi sisitemu ya UNG igomba guhora ikoreshwa mugukwirakwiza PCR, kugirango hirindwe kwanduza ibicuruzwa bya PCR.Ibinyoma byiza.

Birasabwa gukoresha sisitemu ya PCR-UNG ya Forge Biotech mugihe ikora ibizamini binini, nka: Ibiti byamababi Direct PCR Kit-UNG ；

Imbuto y'ibihingwa Direct PCR Kit-UNG ；

Inyama zinyamanswa Direct PCR Kit-UNG ；

Imbeba umurizo Direct PCR Kit-UNG ；

Zebra Ifi Direct PCR Kit-UNG。

Uru ruhererekane rwibikoresho biva muri Foregene ntirushobora gukora PCR gusa byihuse kandi murwego runini, ariko kandi birashobora gukumira no kugenzura ibicuruzwa byanduye PCR.