Gushiraho PCR igerageza SOP kugirango igenzure imyitwarire yabakozi bakora ubushakashatsi.
Ugerageza yubahiriza byimazeyo imikorere yimikorere, kandi agabanya umwanda wa PCR ushobora guterwa nimpamvu zabantu cyangwa ukabuza kwanduza.Byongeye kandi, uwagerageje agomba kuba afite ubumenyi nubuhanga bujyanye n’umwuga, harimo ubuhanga mu bikoresho bifitanye isano no gukora, gusobanura inzira zose z’akazi, kumenya uburyo bwo kuvura bwanduye hamwe n’uburyo bwo kugenzura ubuziranenge bwa laboratoire, kandi agashobora gusobanura neza ibyavuye mu kizamini.
Shiraho laboratoire isanzwe ya PCR.
Laboratoire ya PCR igabanijwemo ibice bine muburyo bwihariye, aribwo agace gategura reagent, agace gatunganyirizwamo icyitegererezo, agace kongerewe imbaraga, hamwe n’isesengura ryibicuruzwa byongerewe imbaraga.Ibice bibiri byambere ni pre-amplification ahantu, naho ibice bibiri byanyuma ni nyuma yinyongera.Agace kibanziriza amplification na zone ya nyuma ya amplification bigomba gutandukana rwose.Ibikoresho byubushakashatsi, reagent, impapuro zafashwe amajwi, amakaramu, ibikoresho byogusukura, nibindi, birashobora gutemba gusa kuva ahantu habanje kwongerwaho imbaraga kugeza ahakurikira nyuma yo gukwirakwizwa, ni ukuvuga kuva ahantu hateganijwe reagent area ahantu ho gutunganyirizwa → agace kongerewe → ahantu hasesengurwa ryibicuruzwa, kandi ntigomba gusubira inyuma.Umwuka uhumeka muri laboratoire ugomba kandi gutemba uva ahantu habanziriza amplifisione ujya nyuma ya amplification, kandi ntusubire inyuma.Igishushanyo cyiza cya PCR cyerekanwe hano hepfo:
Igishushanyo A: Uburyo bwiza bwa PCR bwa laboratoire hamwe numuvuduko mubi mubyumba bya buffer
Igishushanyo B: Uburyo bwiza bwa PCR muri laboratoire hamwe numuvuduko mwiza mubyumba bya buffer
Igishushanyo cya laboratoire ya PCR yatanzwe mu gishushanyo A na Ishusho B igomba kuba uburyo bwiza bwo gushiraho, kandi laboratoire ifite imiterere irashobora kwerekeza kuri ubu buryo bwo gushushanya.Kuri laboratoire zisanzwe, birasabwa ko agace kongererwa PCR hamwe n’ahantu hasesengurwa ibicuruzwa bishobora gutandukana, kandi gufungura igifuniko bigomba kugabanuka uko bishoboka kose ahantu hateganijwe icyitegererezo hamwe n’ahantu hongerewe PCR.Wibuke: Ibicuruzwa nibikoresho byubushakashatsi ahantu hasesengurwa ryibicuruzwa birabujijwe rwose kujyanwa ahantu hateganijwe icyitegererezo hamwe na PCR yongerewe imbaraga.
Niba laboratoire ikora gusa PCR no kumenya, irasabwa gukoresha PCR igereranya PCR aho gukoresha PCR isanzwe.
Ibisubizo bya Fluorescence byerekana PCR birashobora gukusanywa no gusesengurwa nibimenyetso bya fluorescence, kubwibyo rero nta mpamvu yo gufungura umupfundikizo wa electrophoreis nyuma yo kubyitwaramo, birinda kwanduza ibicuruzwa bya PCR biterwa no kumeneka kw'ibicuruzwa biva mu kirere kugira ngo bibe aerosole.Niba wongeyeho umubare wugurura cap mugihe cyo gupakira gel electrophorei, kwanduza aerosol birashoboka.Birasabwa guteza imbere ikoreshwa rya PCR igereranije no gusimbuza buhoro buhoro PCR.
Sisitemu yo kwanduza ibicuruzwa UNG irwanya PCR ikoreshwa mubitekerezo bya PCR.
Sisitemu ikoresha dUTP aho gukoresha dTTP.Nyuma ya PCR reaction, ibicuruzwa byose bya PCR (ibice bya ADN) byashizwemo na dUTP;mugice gikurikiraho cya PCR reaction, enzyme ya UNG yongewe muri sisitemu yashyizwe kuri 37 ° C muminota 5 mbere ya PCR, ishobora gutesha agaciro ibice byose bya ADN birimo dUTP, hanyuma bigakora PCR.Ibi birashobora gukuraho burundu kwanduza aerosol guterwa nibicuruzwa bya PCR.Ingaruka zerekanwa ku gishushanyo gikurikira:
Icyitonderwa: Kuburyo bwa PCR itaziguye, urashobora guhitamo ibicuruzwa byuruhererekane rwibicuruzwa birwanya PCR byanduye bya ForegeneTanga igitekerezo
Kuri laboratoire ikora ibizamini binini bya genotyping, birasabwa cyane gukoresha sisitemu yo kwanduza UNG anti-PCR kugirango yipime reagent hiyubakwa laboratoire zifatika.
Kwibutsa: Gukoresha iyi sisitemu ntibishobora gukuraho ibicuruzwa byanduye PCR bimaze guterwa.Kubwibyo, sisitemu ya UNG igomba gukoreshwa mugitangira ikizamini kijyanye, na sisitemu ya UNG igomba gukoreshwa mugukwirakwiza PCR, kugirango hirindwe kwanduza ibicuruzwa bya PCR Ikinyoma cyiza.
Birasabwa gukoresha sisitemu ya PCR-UNG ya Foregene mugihe ukora ibizamini binini, nka:
Gutera ibibabi bitaziguye PCR Kit-UNG ；
Imbuto y'ibihingwa Direct PCR Kit-UNG ；
Inyama zinyamanswa Direct PCR Kit-UNG ；
Imbeba umurizo Direct PCR Kit-UNG ；
Zebra Ifi Direct PCR Kit-UNG。
Uruhererekane rwibikoresho biva muri Foregenentishobora gukora PCR yihuse gusa kandi murwego runini, ariko kandi irashobora gukumira no kugenzura neza ibicuruzwa bya PCR.
Igihe cyo kohereza: Werurwe-19-2021
