Byanditswe na: CAS Laboratoire ya Pathogen Microbiology na Immunology, Institute of Microbiology, Academy of Science of China
NIBA: 69.504
Ibicuruzwa bifashwa:Akagari Igiteranyo cya RNA Igikoresho cyo kwigunga
Ihuza ry'umwimerere:https://www.umuco.com/articles/s41586-022-05271-2
Kwandura virusi ya virusi nka virusi ya Ebola (EBOV) na virusi ya Marburg birashobora gutera ibimenyetso bikomeye by’amavuriro, harimo umuriro wa haemorhagie ndetse no kunanirwa na multiorgan.4.Hano twagennye imiterere ya poroteyine ya Ebola L muri complexe hamwe na tetrameric VP35 dukoresheje microscopi ya cryo-electron (leta 1).Isesengura ry’imiterere ryagaragaje ko virusi ya Ebola L ifite ikintu cyihariye cya filovirus yinjizamo ingirakamaro muri synthesis ya RNA, kandi ko VP35 ikorana cyane n’akarere ka N-terminal ka L na batatu ba porotokore ya VP35.
Muri iyi nyandiko, RNA yose yakuweho hakoreshejwe ForegeneAkagari Igiteranyo cya RNA Igikoresho cyo kwigungaubunini bwa RNA bwapimwe na Nanodrop kuri 260 nm, hanyuma bukore RT / qPCR.
Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.
Igihe cyo kohereza: Ugushyingo-04-2022
