Amavu n'amavuko
Mu myaka yashize, imitsi idasanzwe (EVS) yakwegereye abantu nkigikoresho gishobora kuvura;icyakora, ingaruka zo kuvura za EV kuri endometriose ntizigeze zitangazwa.Endometriose ni indwara ikunze kwandura indwara z’abagore yibasira 10-15% by’abagore bafite imyaka yo kubyara, bigatera ibimenyetso bitandukanye, bigatuma igabanuka ry’imibereho n’umutwaro munini w’imibereho.
Ingingo Intangiriro
Ku ya 20 Nyakanga 2021, itsinda ry’ubushakashatsi bwa Porofeseri Wang Guoyun wo mu bitaro bya Qilu byo muri kaminuza ya Shandong ryasohoye inyandiko y’ubushakashatsi yise “M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophage yo kubuza iterambere rya Endometriose” ku mipaka muri immunologiya, yaganiriye ku nkomoko ya macrophage ya M1.Ibishoboka bya nanovesicles (NVs) mukuvura endometriose.
Iyi ngingo ikoresha uburyo bukomeza bwo gukuramo M1NVs, kandi ikoresha uburyo bwo guhuza umuco kugirango yige impinduka muri angiogenezi, kwimuka, gutera ndetse nibindi bipimo byerekana ingirabuzimafatizo ya eutopique (EM-ESCs) ituruka ku barwayi bafite endometriose.Muri icyo gihe, hashyizweho urugero rwimbeba ya endometriose, hanyuma imbeba zavuwe na PBS, MONVs cyangwa M1NVs, kugirango zisuzume imikorere n’umutekano bya M1NV mu kuvura endometriose.
Ibisubizo byerekanye ko muri vitro M1NVs zishobora kubuza mu buryo butaziguye cyangwa butaziguye kwimuka no gutera EM-ESCs, kandi bikabuza angiogenez.Muburyo bwimbeba, M1NVs ibuza kubaho kwa endometriose binyuze muri M2 macrophage reprogrammes idateze kwangirika kwingingo.Irerekana ko M1NVs ishobora kubuza mu buryo butaziguye kubaho kwa endometriose, kandi irashobora no guhagarikwa no guhinduranya macrophage yo mu bwoko bwa M2 ku bwoko bwa M1.Kubwibyo, gukoresha M1NVs birashobora kuba uburyo bushya bwo kuvura endometriose.
Ubufasha bwa Foregene
Mu bushakashatsi, kubera ko M1NV yateguwe no guhora ikanda macrophage ya M1, ingingo yakoresheje qRT-PCR kugirango imenye ibintu bitera umuriro hamwe na M1 macrophage marike iNOS, TNF-a na IL-6 mRNA muri M1NV na M1.Umubare ugereranije nimpinduka.Ibisubizo byerekanye ko M1NVs zirimo ibintu byinshi bitera inflammatory mRNA na M1 macrophage, byerekana ko M1NVs zishobora kugumana neza imikorere yimikorere ya selile M1.Ubu buryo bwubushakashatsi bukoresha Akagari kihuse RT-qPCR Kit-SYBR Icyatsi I cya Foregene
Akagari ka RT-qPCR Kitburambuye

Ibisabwa:
 
1. Kugenzura ibisekuru no gusesengura imvugo, kugenzura gene ikabije cyangwa ingaruka zo kwivanga, gusuzuma ibiyobyabwenge, nibindi.;
2. Imvugo yerekana ibisekuruza bigoye-guhingwa nka selile yibanze, selile stem, na selile selile;
3. Kumenya mRNA mubitegererezo nka exosomes na nanovesicles.
Ibiranga: