DirectPCR ni iki?
Tekinoroji ya DirectPCR isobanura ko hatabayeho gutandukanya no kweza molekile ya acide nucleique (harimo ADN na RNA) yingero zinyuranye, gusa ingirangingo nu miterere ya selile byangizwa na lysis ya protease, aside nucleic irekurwa mugisubizo cya lysis, hanyuma igisubizo cya lysis kikongerwaho muburyo butaziguye.Sisitemu ya reaction ya PCR nubuhanga bwo kongera gene igenewe.
Urashaka impinduka
Gutandukana no gukuramo ikibazo cya acide nucleic
Kuva havumburwa ikoranabuhanga rya PCR mu myaka 30 ishize, abashakashatsi bahangayikishijwe no gutandukanya no gukuramo aside nucleic.Gukoresha ingero za tissue kugirango ukore reaction ya PCR ninzozi zabashakashatsi benshi.Ariko izi nzozi ntizimaze imyaka 30 zisohora.Impamvu nuko tissue isenyutse izarekura ibintu byinshi bibuza.
Ibi bikoresho byo kubuza bizagira ingaruka zikomeye zo kubuza PCR kubyitwaramo, bimwe bizatera primer hamwe nicyitegererezo kidashobora guhambira, bimwe bifite imikorere ikomeye ya proteine denaturation, bikavamo kudakora kwa nucleic aside polymerase, kandi bimwe bikabuza primer nicyitegererezo guhuza.Ibi nibintu byose bitera reaction ya PCR idakomeza neza.
Ubusanzwe, abantu rimwe na rimwe bakoresha ibyuma byongera PCR kugirango bongere ibyiyumvo, umwihariko hamwe no kongera imikorere ya PCR.Nyamara, bitewe na inhibitori zitandukanye za PCR zikubiye muri templic acide nucleic zituruka ahantu hatandukanye, gukoresha igihe kirekire umubare munini wabongera imbaraga biragaragara ko bidakwiye igihombo, ikiguzi ni kinini, kandi ibikorwa biragoye.
Foregene DirectPCR
Iterambere ryambere kwisi - Ubuhanga bubiri
Foregene imaze gutera intambwe murwego rwikoranabuhanga kurwego rwisi.Ikoranabuhanga rya Foregene DirectPCR rifite ingingo ebyiri za tekiniki, zifasha Foregene kuba intangiriro nubuyobozi bwikoranabuhanga rya DirectPCR.
Ubwa mbere, patenti nucleic acide polymerase uburyo bwo guhindura.Wibagiwe ifite uburyo bwo guhindura nuclease yemewe, igamije guhuza domaine kugirango nuclease hamwe nicyitegererezo bikomere, hamwe nibikorwa byinshi, gukora neza kwinshi hamwe nubushyuhe bwumuriro.
Icya kabiri, PCR ivanze ya PCR yatezimbere kubwoko butandukanye, ibyongera imbaraga zidasanzwe, optimizers hamwe na stabilisateur, byongera cyane polymerase irwanya inhibitori ya PCR kandi ikanakora neza.
Ni ukubera ko ibintu bibiri byingenzi byavuzwe haruguru byavuzwe haruguru Foregene yamenye DirectPCR nyayo kurwego rwisi.Yaba inyama zisanzwe zinyamanswa, amazi yumubiri, ibimera, amababi cyangwa inama zumuzi, cyangwa nimbuto zibihingwa, abayikoresha barashobora kubona byoroshye PCR yongerewe imbaraga nta gucika kumashanyarazi cyangwa uburyo bwo kweza aside nucleic iruhije.
Kubikoresho bya DirectPCR byuruhererekane rwibimera hamwe nuruhererekane rwinyamanswa byatangijwe, Foregene arashobora kwishimira ko dufite ibipimo ngenderwaho byambere ku isi.Mu bihe biri imbere, Foregene izakomeza gushyira ahagaragara urutonde rwibicuruzwa bigifite ibipimo byerekana imikorere ku isi (cyangwa byihariye).
Kubindi bisobanuro, andika:
http://www.foregene.com/http://www.foreivd.com/
Igihe cyo kohereza: Gashyantare-27-2017
